B-glucans are involved in immune-modulation of THP-1

W. Chanput, M. Reitsma, L. Kleinjans, J.J. Mes, H.F.J. Savelkoul, H.J. Wichers

Research output: Contribution to journalArticleAcademicpeer-review

85 Citations (Scopus)


Scope We aimed to examine different immunological aspects of ß-glucans derived from different food sources (oat, barley and shiitake) on phorbol myristate acetate (PMA)-differentiated THP-1 macrophages. Commercially purified barley ß-glucan (commercial BG) and lentinan were included to compare ß-glucans from the same origin but different degree of purity and processing. Methods and results Chemical composition and molecular weight distribution of ß-glucan samples were determined. Inflammation-related gene expression kinetics (IL-1ß, IL-8, nuclear factor kappa B [NF-¿B] and IL-10) after 3, 6 and 24 h of stimulation with 100 µg/mL ß-glucan were investigated. All tested ß-glucans mildly upregulated the observed inflammation-related genes with differential gene expression patterns. Similar gene expression kinetics, but different fold induction values, was found for the crude ß-glucan extracts and their corresponding commercial forms. Pre-incubation of THP-1 macrophages with ß-glucans prior to lipopolysaccharide (LPS) exposure decreased the induction of inflammation-related genes compared to LPS treatment. No production of nitric oxide (NO) and hydrogen peroxide (H2O2) was detected in ß-glucan stimulated THP-1 macrophages. Phagocytic activity was not different after stimulation by ß-glucan samples. Conclusion Based on these in vitro analyses, it can be concluded that the analysed ß-glucans have varying levels of immunomodulating properties, which are likely related to structure, molecular weight and compositional characteristic of ß-glucan
Original languageEnglish
Pages (from-to)822-833
JournalMolecular Nutrition & Food Research
Issue number5
Publication statusPublished - 2012


  • lactic-acid bacteria
  • factor-kappa-b
  • cell-line
  • cytokine production
  • interferon-gamma
  • dendritic cells
  • receptor
  • lentinan
  • mice
  • lipopolysaccharide


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