Azacytidine and miR156 promote rooting in adult but not in juvenile Arabidopsis tissues

Mehdi Massoumi Bagherabadi, Frans A. Krens, Richard G.F. Visser, Geert-Jan de Klerk

Research output: Contribution to journalArticleAcademicpeer-review

7 Citations (Scopus)

Abstract

Poor adventitious root (AR) formation is a major obstacle in micropropagation and conventional vegetative propagation of many crops. It is affected by many endogenous and exogenous factors. With respect to endogenous factors, the phase change from juvenile to adult has a major influence on AR formation and rooting is usually much reduced or even fully inhibited in adult tissues. It has been reported that the phase change is characterized by an increase in DNA-methylation and a decrease in the expression of microRNA156 (miR156). In this paper, we examined the effect of azacytidine (AzaC) and miR156 on AR formation in adult and juvenile Arabidopsis tissues. To identify the ontogenetic state researchers have used flowering or leaf morphology. We have used the rootability which allows – in contrast with both other characteristics- to examine the ontogenetic state at the cellular level. Overexpression of miR156 promoted only the rooting of adult tissues indicating that the phase change-associated loss in tissues’ competence to develop ARs is also under the control of miR156. Azacytidine inhibits DNA methylation during DNA replication. Azacytidine treatment also promoted AR formation in nonjuvenile tissues but had no or little effect in juvenile tissues. Its addition during seedling growth (by which all tissues become hypomethylated) or during the rooting treatment (by which only those cells become hypomethylated that are generated after taking the explant) are both effective in the promotion of rooting. An AzaC treatment may be useful in tissue culture for crops that are recalcitrant to root.

LanguageEnglish
Pages52-60
JournalJournal of Plant Physiology
Volume208
DOIs
Publication statusPublished - 2017

Fingerprint

Azacitidine
Arabidopsis
rooting
adventitious roots
phase transition
DNA methylation
DNA Methylation
vegetative propagation
DNA replication
crops
tissues
micropropagation
seedling growth
tissue culture
DNA Replication
Seedlings
explants
Mental Competency
researchers
flowering

Keywords

  • Adventitious root formation
  • Arabidopsis thaliana
  • Hypomethylation
  • Juvenile
  • miR156
  • Phase change

Cite this

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title = "Azacytidine and miR156 promote rooting in adult but not in juvenile Arabidopsis tissues",
abstract = "Poor adventitious root (AR) formation is a major obstacle in micropropagation and conventional vegetative propagation of many crops. It is affected by many endogenous and exogenous factors. With respect to endogenous factors, the phase change from juvenile to adult has a major influence on AR formation and rooting is usually much reduced or even fully inhibited in adult tissues. It has been reported that the phase change is characterized by an increase in DNA-methylation and a decrease in the expression of microRNA156 (miR156). In this paper, we examined the effect of azacytidine (AzaC) and miR156 on AR formation in adult and juvenile Arabidopsis tissues. To identify the ontogenetic state researchers have used flowering or leaf morphology. We have used the rootability which allows – in contrast with both other characteristics- to examine the ontogenetic state at the cellular level. Overexpression of miR156 promoted only the rooting of adult tissues indicating that the phase change-associated loss in tissues’ competence to develop ARs is also under the control of miR156. Azacytidine inhibits DNA methylation during DNA replication. Azacytidine treatment also promoted AR formation in nonjuvenile tissues but had no or little effect in juvenile tissues. Its addition during seedling growth (by which all tissues become hypomethylated) or during the rooting treatment (by which only those cells become hypomethylated that are generated after taking the explant) are both effective in the promotion of rooting. An AzaC treatment may be useful in tissue culture for crops that are recalcitrant to root.",
keywords = "Adventitious root formation, Arabidopsis thaliana, Hypomethylation, Juvenile, miR156, Phase change",
author = "{Massoumi Bagherabadi}, Mehdi and Krens, {Frans A.} and Visser, {Richard G.F.} and {de Klerk}, Geert-Jan",
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Azacytidine and miR156 promote rooting in adult but not in juvenile Arabidopsis tissues. / Massoumi Bagherabadi, Mehdi; Krens, Frans A.; Visser, Richard G.F.; de Klerk, Geert-Jan .

In: Journal of Plant Physiology, Vol. 208, 2017, p. 52-60.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - Azacytidine and miR156 promote rooting in adult but not in juvenile Arabidopsis tissues

AU - Massoumi Bagherabadi, Mehdi

AU - Krens, Frans A.

AU - Visser, Richard G.F.

AU - de Klerk, Geert-Jan

PY - 2017

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