Avaliacao do contributo da expressao genética na tolerância à desidratacao em Rosa x hybrida coltivada em humidade relativa elevada

D.R.A. Carvalho, M.W. Vasconcelos, C.F.S. Koning-Boucoiran, F.A. Krens, E. Heuvelink, S.M.P. Carvalho

Research output: Chapter in Book/Report/Conference proceedingConference paper

Abstract

Cultivation of cut flowers with high postharvest longevity is of extreme importance for consumer’s satisfaction. A negative water balance is indicated as the most important factor reducing longevity. This problem is more severe in plants grown at high relative air humidity (i.e. RH > 85%) mainly due to a poor stomatal functioning associated to lower abscisic acid (ABA) concentrations, leading to higher water loss. In this paper it is analysed whether a sensitive cut rose cultivar to high RH show different gene expression related to ABA metabolism when grown at moderate (60%) or high (90%) RH. RNA was isolated from fully developed leaves, harvested 180 min after the beginning of the dark period (stomatal closing stimulus) and the relative gene expression was evaluated in genes involved in: biosynthesis (AAO3 and NCED1), oxidation (CYP707A1 and CYP707A3) and conjugation (UGT75B2) of ABA. The stomatal conductivity in the dark period was 5.6-fold higher in intact plants developed at high RH compared to moderate RH-grown plants, showing the reduced closure capacity of the stomata during darkness. After 4 h of leaflet desiccation, leaflets grown at high RH showed a relative water content (RWC) 69% lower than the moderate RH-grown leaflets, validating the sensitivity of K023 to high RH during growth. At last, from the five studied genes only the expression of CYP707A3 was significantly reduced by high RH, without significant differences found in the other genes. In conclusion, the sensitivity of the genotype K023 to high RH cannot be explained by the expression of the genes evaluated in this study, using leaves harvested 180 min after the beginning of the dark period (closing stimulus). We expect that after transferring the plants to the postharvest conditions, inducing water stress, a more contrasting gene expression among RH levels will appear.
Original languagePortuguese
Title of host publicationProducción Viverística (II): Crecimiento y Desarrollo, Material Vegetal y Propagación
Place of PublicationValencia
Pages132-137
Volume68
Publication statusPublished - 2014
EventVI Jornadas Ibéricas de Horticultura Ornamental: Las Buenas Prácticas en la Horticultura Ornamental, Valencia, Spain -
Duration: 1 Oct 20143 Oct 2014

Conference

ConferenceVI Jornadas Ibéricas de Horticultura Ornamental: Las Buenas Prácticas en la Horticultura Ornamental, Valencia, Spain
Period1/10/143/10/14

Cite this

Carvalho, D. R. A., Vasconcelos, M. W., Koning-Boucoiran, C. F. S., Krens, F. A., Heuvelink, E., & Carvalho, S. M. P. (2014). Avaliacao do contributo da expressao genética na tolerância à desidratacao em Rosa x hybrida coltivada em humidade relativa elevada. In Producción Viverística (II): Crecimiento y Desarrollo, Material Vegetal y Propagación (Vol. 68, pp. 132-137). Valencia.
Carvalho, D.R.A. ; Vasconcelos, M.W. ; Koning-Boucoiran, C.F.S. ; Krens, F.A. ; Heuvelink, E. ; Carvalho, S.M.P. / Avaliacao do contributo da expressao genética na tolerância à desidratacao em Rosa x hybrida coltivada em humidade relativa elevada. Producción Viverística (II): Crecimiento y Desarrollo, Material Vegetal y Propagación. Vol. 68 Valencia, 2014. pp. 132-137
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title = "Avaliacao do contributo da expressao gen{\'e}tica na toler{\^a}ncia {\`a} desidratacao em Rosa x hybrida coltivada em humidade relativa elevada",
abstract = "Cultivation of cut flowers with high postharvest longevity is of extreme importance for consumer’s satisfaction. A negative water balance is indicated as the most important factor reducing longevity. This problem is more severe in plants grown at high relative air humidity (i.e. RH > 85{\%}) mainly due to a poor stomatal functioning associated to lower abscisic acid (ABA) concentrations, leading to higher water loss. In this paper it is analysed whether a sensitive cut rose cultivar to high RH show different gene expression related to ABA metabolism when grown at moderate (60{\%}) or high (90{\%}) RH. RNA was isolated from fully developed leaves, harvested 180 min after the beginning of the dark period (stomatal closing stimulus) and the relative gene expression was evaluated in genes involved in: biosynthesis (AAO3 and NCED1), oxidation (CYP707A1 and CYP707A3) and conjugation (UGT75B2) of ABA. The stomatal conductivity in the dark period was 5.6-fold higher in intact plants developed at high RH compared to moderate RH-grown plants, showing the reduced closure capacity of the stomata during darkness. After 4 h of leaflet desiccation, leaflets grown at high RH showed a relative water content (RWC) 69{\%} lower than the moderate RH-grown leaflets, validating the sensitivity of K023 to high RH during growth. At last, from the five studied genes only the expression of CYP707A3 was significantly reduced by high RH, without significant differences found in the other genes. In conclusion, the sensitivity of the genotype K023 to high RH cannot be explained by the expression of the genes evaluated in this study, using leaves harvested 180 min after the beginning of the dark period (closing stimulus). We expect that after transferring the plants to the postharvest conditions, inducing water stress, a more contrasting gene expression among RH levels will appear.",
author = "D.R.A. Carvalho and M.W. Vasconcelos and C.F.S. Koning-Boucoiran and F.A. Krens and E. Heuvelink and S.M.P. Carvalho",
note = "ISBN 978-84-617-3020-9",
year = "2014",
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volume = "68",
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Carvalho, DRA, Vasconcelos, MW, Koning-Boucoiran, CFS, Krens, FA, Heuvelink, E & Carvalho, SMP 2014, Avaliacao do contributo da expressao genética na tolerância à desidratacao em Rosa x hybrida coltivada em humidade relativa elevada. in Producción Viverística (II): Crecimiento y Desarrollo, Material Vegetal y Propagación. vol. 68, Valencia, pp. 132-137, VI Jornadas Ibéricas de Horticultura Ornamental: Las Buenas Prácticas en la Horticultura Ornamental, Valencia, Spain, 1/10/14.

Avaliacao do contributo da expressao genética na tolerância à desidratacao em Rosa x hybrida coltivada em humidade relativa elevada. / Carvalho, D.R.A.; Vasconcelos, M.W.; Koning-Boucoiran, C.F.S.; Krens, F.A.; Heuvelink, E.; Carvalho, S.M.P.

Producción Viverística (II): Crecimiento y Desarrollo, Material Vegetal y Propagación. Vol. 68 Valencia, 2014. p. 132-137.

Research output: Chapter in Book/Report/Conference proceedingConference paper

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T1 - Avaliacao do contributo da expressao genética na tolerância à desidratacao em Rosa x hybrida coltivada em humidade relativa elevada

AU - Carvalho, D.R.A.

AU - Vasconcelos, M.W.

AU - Koning-Boucoiran, C.F.S.

AU - Krens, F.A.

AU - Heuvelink, E.

AU - Carvalho, S.M.P.

N1 - ISBN 978-84-617-3020-9

PY - 2014

Y1 - 2014

N2 - Cultivation of cut flowers with high postharvest longevity is of extreme importance for consumer’s satisfaction. A negative water balance is indicated as the most important factor reducing longevity. This problem is more severe in plants grown at high relative air humidity (i.e. RH > 85%) mainly due to a poor stomatal functioning associated to lower abscisic acid (ABA) concentrations, leading to higher water loss. In this paper it is analysed whether a sensitive cut rose cultivar to high RH show different gene expression related to ABA metabolism when grown at moderate (60%) or high (90%) RH. RNA was isolated from fully developed leaves, harvested 180 min after the beginning of the dark period (stomatal closing stimulus) and the relative gene expression was evaluated in genes involved in: biosynthesis (AAO3 and NCED1), oxidation (CYP707A1 and CYP707A3) and conjugation (UGT75B2) of ABA. The stomatal conductivity in the dark period was 5.6-fold higher in intact plants developed at high RH compared to moderate RH-grown plants, showing the reduced closure capacity of the stomata during darkness. After 4 h of leaflet desiccation, leaflets grown at high RH showed a relative water content (RWC) 69% lower than the moderate RH-grown leaflets, validating the sensitivity of K023 to high RH during growth. At last, from the five studied genes only the expression of CYP707A3 was significantly reduced by high RH, without significant differences found in the other genes. In conclusion, the sensitivity of the genotype K023 to high RH cannot be explained by the expression of the genes evaluated in this study, using leaves harvested 180 min after the beginning of the dark period (closing stimulus). We expect that after transferring the plants to the postharvest conditions, inducing water stress, a more contrasting gene expression among RH levels will appear.

AB - Cultivation of cut flowers with high postharvest longevity is of extreme importance for consumer’s satisfaction. A negative water balance is indicated as the most important factor reducing longevity. This problem is more severe in plants grown at high relative air humidity (i.e. RH > 85%) mainly due to a poor stomatal functioning associated to lower abscisic acid (ABA) concentrations, leading to higher water loss. In this paper it is analysed whether a sensitive cut rose cultivar to high RH show different gene expression related to ABA metabolism when grown at moderate (60%) or high (90%) RH. RNA was isolated from fully developed leaves, harvested 180 min after the beginning of the dark period (stomatal closing stimulus) and the relative gene expression was evaluated in genes involved in: biosynthesis (AAO3 and NCED1), oxidation (CYP707A1 and CYP707A3) and conjugation (UGT75B2) of ABA. The stomatal conductivity in the dark period was 5.6-fold higher in intact plants developed at high RH compared to moderate RH-grown plants, showing the reduced closure capacity of the stomata during darkness. After 4 h of leaflet desiccation, leaflets grown at high RH showed a relative water content (RWC) 69% lower than the moderate RH-grown leaflets, validating the sensitivity of K023 to high RH during growth. At last, from the five studied genes only the expression of CYP707A3 was significantly reduced by high RH, without significant differences found in the other genes. In conclusion, the sensitivity of the genotype K023 to high RH cannot be explained by the expression of the genes evaluated in this study, using leaves harvested 180 min after the beginning of the dark period (closing stimulus). We expect that after transferring the plants to the postharvest conditions, inducing water stress, a more contrasting gene expression among RH levels will appear.

M3 - Conference paper

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BT - Producción Viverística (II): Crecimiento y Desarrollo, Material Vegetal y Propagación

CY - Valencia

ER -

Carvalho DRA, Vasconcelos MW, Koning-Boucoiran CFS, Krens FA, Heuvelink E, Carvalho SMP. Avaliacao do contributo da expressao genética na tolerância à desidratacao em Rosa x hybrida coltivada em humidade relativa elevada. In Producción Viverística (II): Crecimiento y Desarrollo, Material Vegetal y Propagación. Vol. 68. Valencia. 2014. p. 132-137