Auxin reflux between the endodermis and pericycle promotes lateral root initiation

P. Marhavy; M. Vanstraelen; B.P.M. de Rybel; Z.J. Ding; M.J. Bennett; T. Beeckman; E. Benkova

doi:10.1038/emboj.2012.303

Auxin reflux between the endodermis and pericycle promotes lateral root initiation

P. Marhavy, M. Vanstraelen, B.P.M. de Rybel, Z.J. Ding, M.J. Bennett, T. Beeckman, E. Benkova

Biochemistry

Research output: Contribution to journal › Article › Academic › peer-review

130 Citations (Scopus)

Abstract

Lateral root (LR) formation is initiated when pericycle cells accumulate auxin, thereby acquiring founder cell (FC) status and triggering asymmetric cell divisions, giving rise to a new primordium. How this auxin maximum in pericycle cells builds up and remains focused is not understood. We report that the endodermis plays an active role in the regulation of auxin accumulation and is instructive for FCs to progress during the LR initiation (LRI) phase. We describe the functional importance of a PIN3 (PIN-formed) auxin efflux carrier-dependent hormone reflux pathway between overlaying endodermal and pericycle FCs. Disrupting this reflux pathway causes dramatic defects in the progress of FCs towards the next initiation phase. Our data identify an unexpected regulatory function for the endodermis in LRI as part of the fine-tuning mechanism that appears to act as a check point in LR organogenesis after FCs are specified. The EMBO Journal (2013) 32, 149-158. doi:10.1038/emboj.2012.303; Published online 23 November 2012

Original language	English
Pages (from-to)	149-158
Journal	The EMBO Journal
Volume	32
Issue number	1
DOIs	https://doi.org/10.1038/emboj.2012.303
Publication status	Published - 2013

Keywords

arabidopsis-thaliana
gene-expression
cell-cycle
efflux
transport
family
organization
gradients
proteins
meristem

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10.1038/emboj.2012.303

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abstract = "Lateral root (LR) formation is initiated when pericycle cells accumulate auxin, thereby acquiring founder cell (FC) status and triggering asymmetric cell divisions, giving rise to a new primordium. How this auxin maximum in pericycle cells builds up and remains focused is not understood. We report that the endodermis plays an active role in the regulation of auxin accumulation and is instructive for FCs to progress during the LR initiation (LRI) phase. We describe the functional importance of a PIN3 (PIN-formed) auxin efflux carrier-dependent hormone reflux pathway between overlaying endodermal and pericycle FCs. Disrupting this reflux pathway causes dramatic defects in the progress of FCs towards the next initiation phase. Our data identify an unexpected regulatory function for the endodermis in LRI as part of the fine-tuning mechanism that appears to act as a check point in LR organogenesis after FCs are specified. The EMBO Journal (2013) 32, 149-158. doi:10.1038/emboj.2012.303; Published online 23 November 2012",

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AU - Vanstraelen, M.

AU - de Rybel, B.P.M.

AU - Ding, Z.J.

AU - Bennett, M.J.

AU - Beeckman, T.

AU - Benkova, E.

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N2 - Lateral root (LR) formation is initiated when pericycle cells accumulate auxin, thereby acquiring founder cell (FC) status and triggering asymmetric cell divisions, giving rise to a new primordium. How this auxin maximum in pericycle cells builds up and remains focused is not understood. We report that the endodermis plays an active role in the regulation of auxin accumulation and is instructive for FCs to progress during the LR initiation (LRI) phase. We describe the functional importance of a PIN3 (PIN-formed) auxin efflux carrier-dependent hormone reflux pathway between overlaying endodermal and pericycle FCs. Disrupting this reflux pathway causes dramatic defects in the progress of FCs towards the next initiation phase. Our data identify an unexpected regulatory function for the endodermis in LRI as part of the fine-tuning mechanism that appears to act as a check point in LR organogenesis after FCs are specified. The EMBO Journal (2013) 32, 149-158. doi:10.1038/emboj.2012.303; Published online 23 November 2012

AB - Lateral root (LR) formation is initiated when pericycle cells accumulate auxin, thereby acquiring founder cell (FC) status and triggering asymmetric cell divisions, giving rise to a new primordium. How this auxin maximum in pericycle cells builds up and remains focused is not understood. We report that the endodermis plays an active role in the regulation of auxin accumulation and is instructive for FCs to progress during the LR initiation (LRI) phase. We describe the functional importance of a PIN3 (PIN-formed) auxin efflux carrier-dependent hormone reflux pathway between overlaying endodermal and pericycle FCs. Disrupting this reflux pathway causes dramatic defects in the progress of FCs towards the next initiation phase. Our data identify an unexpected regulatory function for the endodermis in LRI as part of the fine-tuning mechanism that appears to act as a check point in LR organogenesis after FCs are specified. The EMBO Journal (2013) 32, 149-158. doi:10.1038/emboj.2012.303; Published online 23 November 2012

KW - arabidopsis-thaliana

KW - gene-expression

KW - cell-cycle

KW - efflux

KW - transport

KW - family

KW - organization

KW - gradients

KW - proteins

KW - meristem

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DO - 10.1038/emboj.2012.303

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EP - 158

JO - The EMBO Journal

JF - The EMBO Journal

IS - 1

ER -

Auxin reflux between the endodermis and pericycle promotes lateral root initiation

Abstract

Keywords

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