Automation of selective assays for on-line bioprocess monitoring by flowinjection analysis.

On-line analysis of one component in a complex media used for bioprocesses requires the application of selective tests such as enzyme assays. Because these assays are susceptible to interference by other medium components and have a limited detection range, automatic sample pretreatment is a prerequisite. The progress made with automatic sample pretreatment in flow-injection analysis makes this technique particularly suitable for on-line monitoring of bioprocesses. Moreover, newly developed software control systems may improve the necessary robustness of flow-injection analysis systems. On-line analysis of one component in a complex media used for bioprocesses requires the application of selective tests such as enzyme assays. Because these assays are susceptible to interference by other medium components and have a limited detection range, automatic sample pretreatment is a prerequisite. The progress made with automatic sample pretreatment in flow-injection analysis makes this technique particularly suitable for on-line monitoring of bioprocesses. Moreover, newly developed software control systems may improve the necessary robustness of flow-injection analysis systems.