Automated DNA purification and multiplexed lamp assay preparation on a centrifugal microfluidic 'Lab-on-a-Disc' platform

David J. Kinahan; Lourdes A.N. Julius; Cor Schoen; Tanja Dreo; Jens Ducree

doi:10.1109/MEMSYS.2018.8346761

Automated DNA purification and multiplexed lamp assay preparation on a centrifugal microfluidic 'Lab-on-a-Disc' platform

David J. Kinahan^*, Lourdes A.N. Julius, Cor Schoen, Tanja Dreo, Jens Ducree

^*Corresponding author for this work

Biointeractions and Plant Health

Research output: Chapter in Book/Report/Conference proceeding › Conference paper › Academic › peer-review

9 Citations (Scopus)

Abstract

This work presents a rotational-pulse actuated micro-fluidic cartridge enabling automated detection of plant pathogens on a compact device towards point-of-use monitoring of food safety. This highly integrated 'Lab-on-a-Disc' (LoaD) system first runs the sample over a stationary phase of silica beads, followed by ethanol (EtOH) wash and final elution of DNA. The eluate is then homogenized using 'shake mode' agitation, accurately metered and then mixed with reagents for loop-mediated isothermal amplification (LAMP). We successfully purify plant DNA and demonstrate on-disc quantitative LAMP amplification.

Original language	English
Title of host publication	2018 IEEE Micro Electro Mechanical Systems, MEMS 2018
Publisher	IEEE
Pages	1134-1137
Number of pages	4
ISBN (Electronic)	9781538647820
DOIs	https://doi.org/10.1109/MEMSYS.2018.8346761
Publication status	Published - 26 Apr 2018
Event	31st IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2018 - Belfast, United Kingdom Duration: 21 Jan 2018 → 25 Jan 2018

Conference

Conference	31st IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2018
Country/Territory	United Kingdom
City	Belfast
Period	21/01/18 → 25/01/18

Access to Document

10.1109/MEMSYS.2018.8346761

DECATHLON: Development of Cost efficient Advanced DNA-based methods for specific Traceability issues and High Level On-site applicatioNs
1/12/13 → 30/11/16
Project: EU research project

Cite this

@inproceedings{bedfc792bcc742e8a090debc4ffd1ea7,

title = "Automated DNA purification and multiplexed lamp assay preparation on a centrifugal microfluidic 'Lab-on-a-Disc' platform",

abstract = "This work presents a rotational-pulse actuated micro-fluidic cartridge enabling automated detection of plant pathogens on a compact device towards point-of-use monitoring of food safety. This highly integrated 'Lab-on-a-Disc' (LoaD) system first runs the sample over a stationary phase of silica beads, followed by ethanol (EtOH) wash and final elution of DNA. The eluate is then homogenized using 'shake mode' agitation, accurately metered and then mixed with reagents for loop-mediated isothermal amplification (LAMP). We successfully purify plant DNA and demonstrate on-disc quantitative LAMP amplification.",

author = "Kinahan, {David J.} and Julius, {Lourdes A.N.} and Cor Schoen and Tanja Dreo and Jens Ducree",

year = "2018",

month = apr,

day = "26",

doi = "10.1109/MEMSYS.2018.8346761",

language = "English",

pages = "1134--1137",

booktitle = "2018 IEEE Micro Electro Mechanical Systems, MEMS 2018",

publisher = "IEEE",

address = "United States",

note = "31st IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2018 ; Conference date: 21-01-2018 Through 25-01-2018",

}

Kinahan, DJ, Julius, LAN, Schoen, C, Dreo, T & Ducree, J 2018, Automated DNA purification and multiplexed lamp assay preparation on a centrifugal microfluidic 'Lab-on-a-Disc' platform. in 2018 IEEE Micro Electro Mechanical Systems, MEMS 2018. IEEE, pp. 1134-1137, 31st IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2018, Belfast, United Kingdom, 21/01/18. https://doi.org/10.1109/MEMSYS.2018.8346761

Automated DNA purification and multiplexed lamp assay preparation on a centrifugal microfluidic 'Lab-on-a-Disc' platform. / Kinahan, David J.; Julius, Lourdes A.N.; Schoen, Cor et al.
2018 IEEE Micro Electro Mechanical Systems, MEMS 2018. IEEE, 2018. p. 1134-1137.

Research output: Chapter in Book/Report/Conference proceeding › Conference paper › Academic › peer-review

TY - GEN

T1 - Automated DNA purification and multiplexed lamp assay preparation on a centrifugal microfluidic 'Lab-on-a-Disc' platform

AU - Kinahan, David J.

AU - Julius, Lourdes A.N.

AU - Schoen, Cor

AU - Dreo, Tanja

AU - Ducree, Jens

PY - 2018/4/26

Y1 - 2018/4/26

N2 - This work presents a rotational-pulse actuated micro-fluidic cartridge enabling automated detection of plant pathogens on a compact device towards point-of-use monitoring of food safety. This highly integrated 'Lab-on-a-Disc' (LoaD) system first runs the sample over a stationary phase of silica beads, followed by ethanol (EtOH) wash and final elution of DNA. The eluate is then homogenized using 'shake mode' agitation, accurately metered and then mixed with reagents for loop-mediated isothermal amplification (LAMP). We successfully purify plant DNA and demonstrate on-disc quantitative LAMP amplification.

AB - This work presents a rotational-pulse actuated micro-fluidic cartridge enabling automated detection of plant pathogens on a compact device towards point-of-use monitoring of food safety. This highly integrated 'Lab-on-a-Disc' (LoaD) system first runs the sample over a stationary phase of silica beads, followed by ethanol (EtOH) wash and final elution of DNA. The eluate is then homogenized using 'shake mode' agitation, accurately metered and then mixed with reagents for loop-mediated isothermal amplification (LAMP). We successfully purify plant DNA and demonstrate on-disc quantitative LAMP amplification.

U2 - 10.1109/MEMSYS.2018.8346761

DO - 10.1109/MEMSYS.2018.8346761

M3 - Conference paper

AN - SCOPUS:85046995112

SP - 1134

EP - 1137

BT - 2018 IEEE Micro Electro Mechanical Systems, MEMS 2018

PB - IEEE

T2 - 31st IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2018

Y2 - 21 January 2018 through 25 January 2018

ER -

Automated DNA purification and multiplexed lamp assay preparation on a centrifugal microfluidic 'Lab-on-a-Disc' platform

Abstract

Conference

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Projects

DECATHLON: Development of Cost efficient Advanced DNA-based methods for specific Traceability issues and High Level On-site applicatioNs

Cite this