Automated DNA purification and multiplexed lamp assay preparation on a centrifugal microfluidic 'Lab-on-a-Disc' platform

David J. Kinahan*, Lourdes A.N. Julius, Cor Schoen, Tanja Dreo, Jens Ducree

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference paper

4 Citations (Scopus)

Abstract

This work presents a rotational-pulse actuated micro-fluidic cartridge enabling automated detection of plant pathogens on a compact device towards point-of-use monitoring of food safety. This highly integrated 'Lab-on-a-Disc' (LoaD) system first runs the sample over a stationary phase of silica beads, followed by ethanol (EtOH) wash and final elution of DNA. The eluate is then homogenized using 'shake mode' agitation, accurately metered and then mixed with reagents for loop-mediated isothermal amplification (LAMP). We successfully purify plant DNA and demonstrate on-disc quantitative LAMP amplification.
Original languageEnglish
Title of host publication2018 IEEE Micro Electro Mechanical Systems, MEMS 2018
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages1134-1137
Number of pages4
ISBN (Electronic)9781538647820
DOIs
Publication statusPublished - 26 Apr 2018
Event31st IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2018 - Belfast, United Kingdom
Duration: 21 Jan 201825 Jan 2018

Conference

Conference31st IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2018
CountryUnited Kingdom
CityBelfast
Period21/01/1825/01/18

Fingerprint Dive into the research topics of 'Automated DNA purification and multiplexed lamp assay preparation on a centrifugal microfluidic 'Lab-on-a-Disc' platform'. Together they form a unique fingerprint.

  • Projects

    Cite this

    Kinahan, D. J., Julius, L. A. N., Schoen, C., Dreo, T., & Ducree, J. (2018). Automated DNA purification and multiplexed lamp assay preparation on a centrifugal microfluidic 'Lab-on-a-Disc' platform. In 2018 IEEE Micro Electro Mechanical Systems, MEMS 2018 (pp. 1134-1137). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/MEMSYS.2018.8346761