Authentication of Closely Related Fish and Derived Fish Products Using Tandem Mass Spectrometry and Spectral Library Matching

Merel A. Nessen, Dennis J. van der Zwaan, Sander Grevers, Hans Dalebout, Martijn Staats, Esther Kok*, Magnus Palmblad

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

14 Citations (Scopus)

Abstract

Proteomics methodology has seen increased application in food authentication, including tandem mass spectrometry of targeted species-specific peptides in raw, processed, or mixed food products. We have previously described an alternative principle that uses untargeted data acquisition and spectral library matching, essentially spectral counting, to compare and identify samples without the need for genomic sequence information in food species populations. Here, we present an interlaboratory comparison demonstrating how a method based on this principle performs in a realistic context. We also increasingly challenge the method by using data from different types of mass spectrometers, by trying to distinguish closely related and commercially important flatfish, and by analyzing heavily contaminated samples. The method was found to be robust in different laboratories, and 94-97% of the analyzed samples were correctly identified, including all processed and contaminated samples.

Original languageEnglish
Pages (from-to)3669-3677
JournalJournal of Agricultural and Food Chemistry
Volume64
Issue number18
DOIs
Publication statusPublished - 2016

Keywords

  • food authentication
  • mass spectrometry
  • proteomics
  • species identification
  • spectral libraries

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