Asynchronous Supply of Indispensable Amino Acids Reduces Protein Deposition in Milk-Fed Calves

J.J.G.C. van den Borne, S.J.J. Alferink, M.J.W. Heetkamp, A.A.A. Jacobs, M.W.A. Verstegen, W.J.J. Gerrits

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Abstract

A balanced supply of indispensable amino acids (AA) is required for efficient protein synthesis. Different absorption kinetics (e.g., free vs. protein-bound AA) may, however, create asynchrony in postabsorptive availability of individual AA, thereby reducing the efficiency of protein deposition. We studied the effects of AA asynchrony on protein metabolism in growing, milk-fed calves. In 2 experiments, each with a change-over design including 8 calves, a milk replacer deficient in Lys and Thr was used. In Expt. 1, l-Lys and l-Thr were parenterally supplemented, either in synchrony (SYN), asynchrony (ASYN), or partial asynchrony (PART) with dietary AA. In Expt. 2, l-Lys and l-Thr were orally supplemented, either in SYN or ASYN with dietary AA. In Expt. 1, digested protein was used less efficiently for growth for ASYN (31.0%) than for SYN (37.7%), with PART being intermediate (36.0%). Indicator AA oxidation tended (P = 0.06) to be higher for ASYN. In Expt. 2, the efficiency of protein utilization was lower for ASYN (34.9%) than for SYN (46.6%). Calves spared AA from oxidation when the limiting AA were provided in excess after a short period (
Original languageEnglish
Pages (from-to)2075-2082
JournalThe Journal of Nutrition
Volume142
Issue number12
DOIs
Publication statusPublished - 2012

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Keywords

  • bound lysine
  • growing-pigs
  • preruminant calves
  • alpha-ketoisocaproate
  • leucine oxidation
  • balance technique
  • healthy-adults
  • delayed lysine
  • fat deposition
  • wheat gluten

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