Astin C Production by the Endophytic Fungus Cyanodermella asteris in Planktonic and Immobilized Culture Conditions

Antoine Vassaux, Cédric Tarayre, Anthony Arguëlles-Arias, Philippe Compère, Frank Delvigne, Patrick Fickers, Linda Jahn, Alexander Lang, Valérie Leclère, Jutta Ludwig-Müller, Marc Ongena, Thomas Schafhauser, Samuel Telek, Ariane Théatre, Willem J.H. van Berkel, Micheline Vandenbol, Karl Heinz van Pée, Luc Willems, Wolfgang Wohlleben, Philippe Jacques*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

4 Citations (Scopus)


The fungal endophyte Cyanodermella asteris (C. asteris) has been recently isolated from the medicinal plant Aster tataricus (A. tataricus). This fungus produces astin C, a cyclic pentapeptide with anticancer and anti-inflammatory properties. The production of this secondary metabolite is compared in immobilized and planktonic conditions. For immobilized cultures, a stainless steel packing immersed in the culture broth is used as a support. In these conditions, the fungus exclusively grows on the packing, which provides a considerable advantage for astin C recovery and purification. C. asteris metabolism is different according to the culture conditions in terms of substrate consumption rate, cell growth, and astin C production. Immobilized-cell cultures yield a 30% increase of astin C production, associated with a 39% increase in biomass. The inoculum type as spores rather than hyphae, and a pre-inoculation washing procedure with sodium hydroxide, turns out to be beneficial both for astin C production and fungus development onto the support. Finally, the influence of culture parameters such as pH and medium composition on astin C production is evaluated. With optimized culture conditions, astin C yield is further improved reaching a five times higher final specific yield compared to the value reported with astin C extraction from A. tataricus (0.89 mg g−1 and 0.16 mg g−1 respectively).

Original languageEnglish
Article number1800624
JournalBiotechnology Journal
Issue number8
Publication statusPublished - 1 Aug 2019


  • astin C
  • biofilms
  • Cyanodermella asteris
  • immobilized-cell cultures
  • secondary metabolites


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