Association mapping of salt tolerance in barley (Hordeum vulgare L.)

L. Nguyen Viet Long, O. Dolstra, M. Malosetti, B. Kilian, A. Graner, R.G.F. Visser, C.G. van der Linden

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67 Citations (Scopus)

Abstract

A spring barley collection of 192 genotypes from a wide geographical range was used to identify quantitative trait loci (QTLs) for salt tolerance traits by means of an association mapping approach using a thousand SNP marker set. Linkage disequilibrium (LD) decay was found with marker distances spanning 2–8 cM depending on the methods used to account for population structure and genetic relatedness between genotypes. The association panel showed large variation for traits that were highly heritable under salt stress, including biomass production, chlorophyll content, plant height, tiller number, leaf senescence and shoot Na+, shoot Cl- and shoot, root Na+/K+ contents. The significant correlations between these traits and salt tolerance (defined as the biomass produced under salt stress relative to the biomass produced under control conditions) indicate that these traits contribute to (components of) salt tolerance. Association mapping was performed using several methods to account for population structure and minimize false-positive associations. This resulted in the identification of a number of genomic regions that strongly influenced salt tolerance and ion homeostasis, with a major QTL controlling salt tolerance on chromosome 6H, and a strong QTL for ion contents on chromosome 4H.
Original languageEnglish
Pages (from-to)2335-2351
JournalTheoretical and Applied Genetics
Volume126
Issue number9
DOIs
Publication statusPublished - 2013

Keywords

  • genome-wide association
  • abiotic stress tolerance
  • quantitative trait loci
  • linkage disequilibrium
  • population-structure
  • salinity stress
  • expression analysis
  • ion homeostasis
  • wild barley
  • molecular markers

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