Association mapping and genetic dissection of drought-induced canopy temperature differences in rice

Giovanni Melandri, Ankush Prashar, Susan R. Mccouch, Gerard Van Der Linden, Hamlyn G. Jones, Niteen Kadam, Krishna Jagadish, Harro Bouwmeester*, Carolien Ruyter-Spira, Om Dhankher (Editor)

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Drought-stressed plants display reduced stomatal conductance, which results in increased leaf temperature by limiting transpiration. In this study, thermal imaging was used to quantify the differences in canopy temperature under drought in a rice diversity panel consisting of 293 indica accessions. The population was grown under paddy field conditions and drought stress was imposed for 2 weeks at flowering. The canopy temperature of the accessions during stress negatively correlated with grain yield (r= –0.48) and positively with plant height (r=0.56). Temperature values were used to perform a genome-wide association (GWA) analysis using a 45K single nucleotide polynmorphism (SNP) map. A quantitative trait locus (QTL) for canopy temperature under drought was detected on chromosome 3 and fine-mapped using a high-density imputed SNP map. The candidate genes underlying the QTL point towards differences in the regulation of guard cell solute intake for stomatal opening as the possible source of temperature variation. Genetic variation for the significant markers of the QTL was present only within the tall, low-yielding landraces adapted to drought-prone environments. The absence of variation in the shorter genotypes, which showed lower leaf temperature and higher grain yield, suggests that breeding for high grain yield in rice under paddy conditions has reduced genetic variation for stomatal response under drought.
Original languageEnglish
Article numbererz527
Pages (from-to)1614–1627
JournalJournal of Experimental Botany
Volume71
Issue number4
Early online date17 Dec 2019
DOIs
Publication statusPublished - Feb 2020

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Droughts
chromosome mapping
Dissection
drought
canopy
rice
Temperature
Quantitative Trait Loci
temperature
quantitative trait loci
grain yield
paddies
Nucleotides
nucleotides
genetic variation
Chromosomes, Human, Pair 3
Genome-Wide Association Study
guard cells
Oryza
landraces

Cite this

Melandri, G., Prashar, A., Mccouch, S. R., Van Der Linden, G., Jones, H. G., Kadam, N., ... Dhankher, O. (Ed.) (2020). Association mapping and genetic dissection of drought-induced canopy temperature differences in rice. Journal of Experimental Botany, 71(4), 1614–1627. [erz527]. https://doi.org/10.1093/jxb/erz527
Melandri, Giovanni ; Prashar, Ankush ; Mccouch, Susan R. ; Van Der Linden, Gerard ; Jones, Hamlyn G. ; Kadam, Niteen ; Jagadish, Krishna ; Bouwmeester, Harro ; Ruyter-Spira, Carolien ; Dhankher, Om (Editor). / Association mapping and genetic dissection of drought-induced canopy temperature differences in rice. In: Journal of Experimental Botany. 2020 ; Vol. 71, No. 4. pp. 1614–1627.
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abstract = "Drought-stressed plants display reduced stomatal conductance, which results in increased leaf temperature by limiting transpiration. In this study, thermal imaging was used to quantify the differences in canopy temperature under drought in a rice diversity panel consisting of 293 indica accessions. The population was grown under paddy field conditions and drought stress was imposed for 2 weeks at flowering. The canopy temperature of the accessions during stress negatively correlated with grain yield (r= –0.48) and positively with plant height (r=0.56). Temperature values were used to perform a genome-wide association (GWA) analysis using a 45K single nucleotide polynmorphism (SNP) map. A quantitative trait locus (QTL) for canopy temperature under drought was detected on chromosome 3 and fine-mapped using a high-density imputed SNP map. The candidate genes underlying the QTL point towards differences in the regulation of guard cell solute intake for stomatal opening as the possible source of temperature variation. Genetic variation for the significant markers of the QTL was present only within the tall, low-yielding landraces adapted to drought-prone environments. The absence of variation in the shorter genotypes, which showed lower leaf temperature and higher grain yield, suggests that breeding for high grain yield in rice under paddy conditions has reduced genetic variation for stomatal response under drought.",
author = "Giovanni Melandri and Ankush Prashar and Mccouch, {Susan R.} and {Van Der Linden}, Gerard and Jones, {Hamlyn G.} and Niteen Kadam and Krishna Jagadish and Harro Bouwmeester and Carolien Ruyter-Spira and Om Dhankher",
year = "2020",
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Melandri, G, Prashar, A, Mccouch, SR, Van Der Linden, G, Jones, HG, Kadam, N, Jagadish, K, Bouwmeester, H, Ruyter-Spira, C & Dhankher, O (ed.) 2020, 'Association mapping and genetic dissection of drought-induced canopy temperature differences in rice', Journal of Experimental Botany, vol. 71, no. 4, erz527, pp. 1614–1627. https://doi.org/10.1093/jxb/erz527

Association mapping and genetic dissection of drought-induced canopy temperature differences in rice. / Melandri, Giovanni; Prashar, Ankush; Mccouch, Susan R.; Van Der Linden, Gerard; Jones, Hamlyn G.; Kadam, Niteen; Jagadish, Krishna; Bouwmeester, Harro; Ruyter-Spira, Carolien; Dhankher, Om (Editor).

In: Journal of Experimental Botany, Vol. 71, No. 4, erz527, 02.2020, p. 1614–1627.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Association mapping and genetic dissection of drought-induced canopy temperature differences in rice

AU - Melandri, Giovanni

AU - Prashar, Ankush

AU - Mccouch, Susan R.

AU - Van Der Linden, Gerard

AU - Jones, Hamlyn G.

AU - Kadam, Niteen

AU - Jagadish, Krishna

AU - Bouwmeester, Harro

AU - Ruyter-Spira, Carolien

A2 - Dhankher, Om

PY - 2020/2

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N2 - Drought-stressed plants display reduced stomatal conductance, which results in increased leaf temperature by limiting transpiration. In this study, thermal imaging was used to quantify the differences in canopy temperature under drought in a rice diversity panel consisting of 293 indica accessions. The population was grown under paddy field conditions and drought stress was imposed for 2 weeks at flowering. The canopy temperature of the accessions during stress negatively correlated with grain yield (r= –0.48) and positively with plant height (r=0.56). Temperature values were used to perform a genome-wide association (GWA) analysis using a 45K single nucleotide polynmorphism (SNP) map. A quantitative trait locus (QTL) for canopy temperature under drought was detected on chromosome 3 and fine-mapped using a high-density imputed SNP map. The candidate genes underlying the QTL point towards differences in the regulation of guard cell solute intake for stomatal opening as the possible source of temperature variation. Genetic variation for the significant markers of the QTL was present only within the tall, low-yielding landraces adapted to drought-prone environments. The absence of variation in the shorter genotypes, which showed lower leaf temperature and higher grain yield, suggests that breeding for high grain yield in rice under paddy conditions has reduced genetic variation for stomatal response under drought.

AB - Drought-stressed plants display reduced stomatal conductance, which results in increased leaf temperature by limiting transpiration. In this study, thermal imaging was used to quantify the differences in canopy temperature under drought in a rice diversity panel consisting of 293 indica accessions. The population was grown under paddy field conditions and drought stress was imposed for 2 weeks at flowering. The canopy temperature of the accessions during stress negatively correlated with grain yield (r= –0.48) and positively with plant height (r=0.56). Temperature values were used to perform a genome-wide association (GWA) analysis using a 45K single nucleotide polynmorphism (SNP) map. A quantitative trait locus (QTL) for canopy temperature under drought was detected on chromosome 3 and fine-mapped using a high-density imputed SNP map. The candidate genes underlying the QTL point towards differences in the regulation of guard cell solute intake for stomatal opening as the possible source of temperature variation. Genetic variation for the significant markers of the QTL was present only within the tall, low-yielding landraces adapted to drought-prone environments. The absence of variation in the shorter genotypes, which showed lower leaf temperature and higher grain yield, suggests that breeding for high grain yield in rice under paddy conditions has reduced genetic variation for stomatal response under drought.

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DO - 10.1093/jxb/erz527

M3 - Article

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SP - 1614

EP - 1627

JO - Journal of Experimental Botany

JF - Journal of Experimental Botany

SN - 0022-0957

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M1 - erz527

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