Assignment of allelic configuration in polyploids using the MAC-PR (microsatellite DNA allele counting peak ratios) method

G.D. Esselink, H. Nybom, B.J. Vosman

    Research output: Contribution to journalArticleAcademicpeer-review

    189 Citations (Scopus)

    Abstract

    Polysomic inheritance frequently results in the simultaneous occurrence of several microsatellite DNA alleles on a single locus. The MAC-PR (microsatellite DNA allele counting¿peak ratios) method was recently developed for the analysis of polyploid plants and makes use of the quantitative values for microsatellite allele peak areas. To date, this approach has only been used in plants with known genetic relationships. We report here the application of MAC-PR for the first time to random samples of unknown pedigrees. We analysed six microsatellite loci using a set of tetraploid ornamental rose (Rosa x hybrida L.) varieties. For each locus, all alleles were analysed in pairwise combinations in order to determine their copy number in the individual samples. This was accomplished by calculating the ratios between the peak areas for two alleles in all of the samples where these two alleles occurred together. The allele peak ratios observed were plotted in a histogram, and those histograms that produced at least two well-separated groups were selected for further analysis. Mean allelic peak ratio values for these groups were compared to the relationships expected between alleles in hypothetical configurations of the locus investigated. Using this approach, we were able to assign precise allelic configurations (the actual genotype) to almost all of the varieties analysed for five of the six loci investigated. MAC-PR also appears to be a very effective tool for detecting null alleles in polyploid species
    Original languageEnglish
    Pages (from-to)402-408
    JournalTheoretical and Applied Genetics
    Volume109
    Issue number2
    DOIs
    Publication statusPublished - 2004

    Keywords

    • potato solanum-tuberosum
    • markers
    • l.
    • identification
    • inheritance
    • population
    • rosaceae
    • rapd
    • aflp
    • ssr

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