Assessment of The Uniformity of Wheat and Tomato Varieties at DNA Microsatellite Loci

R.J. Cooke, G.M.M. Bredemeijer, M.W. Ganal, R. Peeters, P. Isaac, S. Rendell, J. Jackson, B. Vosman

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    Abstract

    By analysing a number (20-38) of individuals from selected varieties of wheat and tomato, we have been able to assess intra-varietal uniformity at certain microsatellite (simple sequence repeat, SSR) loci. In total, 45 varieties of wheat were analysed at between 7-9 different SSR loci, and 10 varieties of tomato were analysed at six loci. The results showed that there was variation both between varieties and between microsatellites in the degree of non-uniformity observed, and it was possible to identify a number of different probable sources of non-uniformity. Twenty-four of the wheat varieties and nine of the tomato varieties were sufficiently uniform to meet the standards currently applied for distinctness, uniformity and stability (DUS) testing using phenotypic characteristics. The implications for the potential future use of SSRs in DUS testing are discussed
    Original languageEnglish
    Pages (from-to)331-341
    JournalEuphytica
    Volume132
    Issue number3
    DOIs
    Publication statusPublished - 2003

    Keywords

    • construction
    • database
    • markers

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  • Cite this

    Cooke, R. J., Bredemeijer, G. M. M., Ganal, M. W., Peeters, R., Isaac, P., Rendell, S., Jackson, J., & Vosman, B. (2003). Assessment of The Uniformity of Wheat and Tomato Varieties at DNA Microsatellite Loci. Euphytica, 132(3), 331-341. https://doi.org/10.1023/A:1025046919570