Assessment of the toxicity of the solid coating PV1 in a marine invironment, using biotests with algae, a rotifer and a bacteria

Research output: Book/ReportReportProfessional


The toxic potential of substances that may leach from the solid coating PV1 was tested using • the marine bacteria Vibrio fisheri in the Microtox® Basic test • the marine algae Skeletonema costatum in a 72h algal growth inhibition test • the marine rotifer Brachionus plicatilis in the 24 h ROTOX® test The tests were performed with the water accommodated fraction (WAF) of the coating in natural seawater. This WAF was produced following a method that is commonly applied for the production of WAFs in toxicity testing with oil. Solid coating material that was scrapped of a metal plate was mixed for 24 hours in filtered natural seawater in a ratio of 1 mg coating per 1 ml seawater. For a normal coated surface this is the equivalent of 4 ml seawater per 1 cm2 coated surface. The coating material was removed from the solution before exposure in the ecotoxicity tests. A control sample was prepared by collecting the material that was scrapped of a 20 cm2 area of a metal plate that was not coated with PV1, but underwent the same pre-treatment (phosphated) as the plate that was actually coated for the production of the test sample. This control sample was tested in the bacteria test and in the rotifer test. The algal growth test required more test material, that was therefore scraped from a metal plate with a relatively thick layer of coating. The scraping was performed in such a way that not the whole coating was removed and thus no control sample was necessary in this test.
Original languageEnglish
Place of PublicationIJmuiden
PublisherIMARES Texel
Number of pages19
Publication statusPublished - 2007

Publication series

NameRapport / Wageningen IMARES
PublisherIMARES Texel C059/07


  • coatings
  • marine environment
  • toxic substances
  • bioassays
  • leaching
  • bacteria
  • algae
  • rotifera
  • aquatic toxicology

Fingerprint Dive into the research topics of 'Assessment of the toxicity of the solid coating PV1 in a marine invironment, using biotests with algae, a rotifer and a bacteria'. Together they form a unique fingerprint.

  • Cite this