This report presents a new approach to studying the metabolic and kinetic properties of anaerobic sludge from single batch experiments. The two main features of the method are that the methane production is measured on-line with a relatively cheap system, and that the methane production data can be plotted as rate vs time curves. The case studies of specific methanogenic activity, biodegradability and toxicity tests here presented show that very accurate kinetic data can be obtained. The method is specifically useful in experiments in which strong changes in methane production occur, and it is proposed as a powerful tool to study methanogenic systems. Furthermore, the method is simple and could be implemented by industry in the routine analysis of sludge.
Gonzalez-Gil, G., Kleerebezem, R., & Lettinga, G. (2002). Assessment of metabolic properties and kinetic parameters of methanogenic sludge by on-line methane production rate measurements. Applied Microbiology and Biotechnology, 58, 248-254. https://doi.org/10.1007/s00253-001-0831-5