Assessment of inflammatory resilience in healthy subjects using dietary lipid and glucose challenges

S. Wopereis, D. Wolvers, M. van Erk, M. Gribnau, B. Kremer, F.A. van Dorsten, E. Boelsma, U. Garczarek, N. Cnubben, L. Frenken, P. van der Logt, H.F.J. Hendriks, R. Albers, J.P.M. van Duynhoven, B. van Ommen, D.M. Jacobs

Research output: Contribution to journalArticleAcademicpeer-review

24 Citations (Scopus)

Abstract

Background Resilience or the ability of our body to cope with daily-life challenges has been proposed as a new definition of health, with restoration of homeostasis as target resultant of various physiological stress responses. Challenge models may thus be a sensitive measure to study the body’s health. The objective of this study was to select a dietary challenge model for the assessment of inflammatory resilience. Meals are a challenge to metabolic homeostasis and are suggested to affect inflammatory pathways, yet data in literature are limited and inconsistent. Method The kinetic responses of three different dietary challenges and a water control challenge were assessed on various metabolic and inflammatory markers in 14 healthy males and females using a full cross-over study design. The dietary challenges included glucose (75 g glucose in 300 ml water), lipids (200 ml whipping cream) and a mix of glucose and lipids (same amounts as above), respectively. Blood samples were collected at baseline and at 0.5, 1, 2, 4, 6, 8 and 10 h after consumption of the treatment products. Inflammation (IFN¿, IL-1ß, IL-6, IL-8, IL-10, IL-12p70, TNF-a CRP, ICAM-1, VCAM-1, SAA, E-selectin, P-selectin, thrombomodulin, leukocytes, neutrophils, lymphocytes) and clinical (e.g. glucose, insulin, triglycerides) markers as well as gene expression in blood cells and plasma oxylipin profiles were measured. Results All three dietary challenges induced changes related to metabolic control such as increases in glucose and insulin after the glucose challenge and increases in triglycerides after the lipid challenge. In addition, differences between the challenges were observed for precursor oxylipins and some downstream metabolites including DiHETrE’s and HODE’s. However, none of the dietary challenges induced an acute inflammatory response, except for a modest increase in circulating leukocyte numbers after the glucose and mix challenges. Furthermore, subtle, yet statistically significant increases in vascular inflammatory markers (sICAM-1 and sVCAM-1) were found after the mix challenge, when compared to the water control challenge. Conclusions This study shows that dietary glucose and lipid challenges did not induce a strong acute inflammatory response in healthy subjects, as quantified by an accurate and broad panel of parameters.
Original languageEnglish
Article number44
Number of pages16
JournalBMC Medical Genomics
Volume6
DOIs
Publication statusPublished - 2013

Fingerprint

Healthy Volunteers
Lipids
Glucose
Oxylipins
Cross-Over Studies
Water
Triglycerides
Homeostasis
Insulin
Thrombomodulin
Physiological Stress
P-Selectin
E-Selectin
Vascular Cell Adhesion Molecule-1
Health
Intercellular Adhesion Molecule-1
Interleukin-8
Interleukin-1
Leukocyte Count
Interleukin-10

Keywords

  • type-2 diabetic-patients
  • necrosis-factor-alpha
  • oxidative stress
  • endothelial dysfunction
  • cytokine concentrations
  • postprandial variations
  • fatty-acids
  • obese men
  • fish-oil
  • markers

Cite this

Wopereis, S., Wolvers, D., van Erk, M., Gribnau, M., Kremer, B., van Dorsten, F. A., ... Jacobs, D. M. (2013). Assessment of inflammatory resilience in healthy subjects using dietary lipid and glucose challenges. BMC Medical Genomics, 6, [44]. https://doi.org/10.1186/1755-8794-6-44
Wopereis, S. ; Wolvers, D. ; van Erk, M. ; Gribnau, M. ; Kremer, B. ; van Dorsten, F.A. ; Boelsma, E. ; Garczarek, U. ; Cnubben, N. ; Frenken, L. ; van der Logt, P. ; Hendriks, H.F.J. ; Albers, R. ; van Duynhoven, J.P.M. ; van Ommen, B. ; Jacobs, D.M. / Assessment of inflammatory resilience in healthy subjects using dietary lipid and glucose challenges. In: BMC Medical Genomics. 2013 ; Vol. 6.
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title = "Assessment of inflammatory resilience in healthy subjects using dietary lipid and glucose challenges",
abstract = "Background Resilience or the ability of our body to cope with daily-life challenges has been proposed as a new definition of health, with restoration of homeostasis as target resultant of various physiological stress responses. Challenge models may thus be a sensitive measure to study the body’s health. The objective of this study was to select a dietary challenge model for the assessment of inflammatory resilience. Meals are a challenge to metabolic homeostasis and are suggested to affect inflammatory pathways, yet data in literature are limited and inconsistent. Method The kinetic responses of three different dietary challenges and a water control challenge were assessed on various metabolic and inflammatory markers in 14 healthy males and females using a full cross-over study design. The dietary challenges included glucose (75 g glucose in 300 ml water), lipids (200 ml whipping cream) and a mix of glucose and lipids (same amounts as above), respectively. Blood samples were collected at baseline and at 0.5, 1, 2, 4, 6, 8 and 10 h after consumption of the treatment products. Inflammation (IFN¿, IL-1{\ss}, IL-6, IL-8, IL-10, IL-12p70, TNF-a CRP, ICAM-1, VCAM-1, SAA, E-selectin, P-selectin, thrombomodulin, leukocytes, neutrophils, lymphocytes) and clinical (e.g. glucose, insulin, triglycerides) markers as well as gene expression in blood cells and plasma oxylipin profiles were measured. Results All three dietary challenges induced changes related to metabolic control such as increases in glucose and insulin after the glucose challenge and increases in triglycerides after the lipid challenge. In addition, differences between the challenges were observed for precursor oxylipins and some downstream metabolites including DiHETrE’s and HODE’s. However, none of the dietary challenges induced an acute inflammatory response, except for a modest increase in circulating leukocyte numbers after the glucose and mix challenges. Furthermore, subtle, yet statistically significant increases in vascular inflammatory markers (sICAM-1 and sVCAM-1) were found after the mix challenge, when compared to the water control challenge. Conclusions This study shows that dietary glucose and lipid challenges did not induce a strong acute inflammatory response in healthy subjects, as quantified by an accurate and broad panel of parameters.",
keywords = "type-2 diabetic-patients, necrosis-factor-alpha, oxidative stress, endothelial dysfunction, cytokine concentrations, postprandial variations, fatty-acids, obese men, fish-oil, markers",
author = "S. Wopereis and D. Wolvers and {van Erk}, M. and M. Gribnau and B. Kremer and {van Dorsten}, F.A. and E. Boelsma and U. Garczarek and N. Cnubben and L. Frenken and {van der Logt}, P. and H.F.J. Hendriks and R. Albers and {van Duynhoven}, J.P.M. and {van Ommen}, B. and D.M. Jacobs",
year = "2013",
doi = "10.1186/1755-8794-6-44",
language = "English",
volume = "6",
journal = "BMC Medical Genomics",
issn = "1755-8794",
publisher = "Springer Verlag",

}

Wopereis, S, Wolvers, D, van Erk, M, Gribnau, M, Kremer, B, van Dorsten, FA, Boelsma, E, Garczarek, U, Cnubben, N, Frenken, L, van der Logt, P, Hendriks, HFJ, Albers, R, van Duynhoven, JPM, van Ommen, B & Jacobs, DM 2013, 'Assessment of inflammatory resilience in healthy subjects using dietary lipid and glucose challenges', BMC Medical Genomics, vol. 6, 44. https://doi.org/10.1186/1755-8794-6-44

Assessment of inflammatory resilience in healthy subjects using dietary lipid and glucose challenges. / Wopereis, S.; Wolvers, D.; van Erk, M.; Gribnau, M.; Kremer, B.; van Dorsten, F.A.; Boelsma, E.; Garczarek, U.; Cnubben, N.; Frenken, L.; van der Logt, P.; Hendriks, H.F.J.; Albers, R.; van Duynhoven, J.P.M.; van Ommen, B.; Jacobs, D.M.

In: BMC Medical Genomics, Vol. 6, 44, 2013.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Assessment of inflammatory resilience in healthy subjects using dietary lipid and glucose challenges

AU - Wopereis, S.

AU - Wolvers, D.

AU - van Erk, M.

AU - Gribnau, M.

AU - Kremer, B.

AU - van Dorsten, F.A.

AU - Boelsma, E.

AU - Garczarek, U.

AU - Cnubben, N.

AU - Frenken, L.

AU - van der Logt, P.

AU - Hendriks, H.F.J.

AU - Albers, R.

AU - van Duynhoven, J.P.M.

AU - van Ommen, B.

AU - Jacobs, D.M.

PY - 2013

Y1 - 2013

N2 - Background Resilience or the ability of our body to cope with daily-life challenges has been proposed as a new definition of health, with restoration of homeostasis as target resultant of various physiological stress responses. Challenge models may thus be a sensitive measure to study the body’s health. The objective of this study was to select a dietary challenge model for the assessment of inflammatory resilience. Meals are a challenge to metabolic homeostasis and are suggested to affect inflammatory pathways, yet data in literature are limited and inconsistent. Method The kinetic responses of three different dietary challenges and a water control challenge were assessed on various metabolic and inflammatory markers in 14 healthy males and females using a full cross-over study design. The dietary challenges included glucose (75 g glucose in 300 ml water), lipids (200 ml whipping cream) and a mix of glucose and lipids (same amounts as above), respectively. Blood samples were collected at baseline and at 0.5, 1, 2, 4, 6, 8 and 10 h after consumption of the treatment products. Inflammation (IFN¿, IL-1ß, IL-6, IL-8, IL-10, IL-12p70, TNF-a CRP, ICAM-1, VCAM-1, SAA, E-selectin, P-selectin, thrombomodulin, leukocytes, neutrophils, lymphocytes) and clinical (e.g. glucose, insulin, triglycerides) markers as well as gene expression in blood cells and plasma oxylipin profiles were measured. Results All three dietary challenges induced changes related to metabolic control such as increases in glucose and insulin after the glucose challenge and increases in triglycerides after the lipid challenge. In addition, differences between the challenges were observed for precursor oxylipins and some downstream metabolites including DiHETrE’s and HODE’s. However, none of the dietary challenges induced an acute inflammatory response, except for a modest increase in circulating leukocyte numbers after the glucose and mix challenges. Furthermore, subtle, yet statistically significant increases in vascular inflammatory markers (sICAM-1 and sVCAM-1) were found after the mix challenge, when compared to the water control challenge. Conclusions This study shows that dietary glucose and lipid challenges did not induce a strong acute inflammatory response in healthy subjects, as quantified by an accurate and broad panel of parameters.

AB - Background Resilience or the ability of our body to cope with daily-life challenges has been proposed as a new definition of health, with restoration of homeostasis as target resultant of various physiological stress responses. Challenge models may thus be a sensitive measure to study the body’s health. The objective of this study was to select a dietary challenge model for the assessment of inflammatory resilience. Meals are a challenge to metabolic homeostasis and are suggested to affect inflammatory pathways, yet data in literature are limited and inconsistent. Method The kinetic responses of three different dietary challenges and a water control challenge were assessed on various metabolic and inflammatory markers in 14 healthy males and females using a full cross-over study design. The dietary challenges included glucose (75 g glucose in 300 ml water), lipids (200 ml whipping cream) and a mix of glucose and lipids (same amounts as above), respectively. Blood samples were collected at baseline and at 0.5, 1, 2, 4, 6, 8 and 10 h after consumption of the treatment products. Inflammation (IFN¿, IL-1ß, IL-6, IL-8, IL-10, IL-12p70, TNF-a CRP, ICAM-1, VCAM-1, SAA, E-selectin, P-selectin, thrombomodulin, leukocytes, neutrophils, lymphocytes) and clinical (e.g. glucose, insulin, triglycerides) markers as well as gene expression in blood cells and plasma oxylipin profiles were measured. Results All three dietary challenges induced changes related to metabolic control such as increases in glucose and insulin after the glucose challenge and increases in triglycerides after the lipid challenge. In addition, differences between the challenges were observed for precursor oxylipins and some downstream metabolites including DiHETrE’s and HODE’s. However, none of the dietary challenges induced an acute inflammatory response, except for a modest increase in circulating leukocyte numbers after the glucose and mix challenges. Furthermore, subtle, yet statistically significant increases in vascular inflammatory markers (sICAM-1 and sVCAM-1) were found after the mix challenge, when compared to the water control challenge. Conclusions This study shows that dietary glucose and lipid challenges did not induce a strong acute inflammatory response in healthy subjects, as quantified by an accurate and broad panel of parameters.

KW - type-2 diabetic-patients

KW - necrosis-factor-alpha

KW - oxidative stress

KW - endothelial dysfunction

KW - cytokine concentrations

KW - postprandial variations

KW - fatty-acids

KW - obese men

KW - fish-oil

KW - markers

U2 - 10.1186/1755-8794-6-44

DO - 10.1186/1755-8794-6-44

M3 - Article

VL - 6

JO - BMC Medical Genomics

JF - BMC Medical Genomics

SN - 1755-8794

M1 - 44

ER -