Assessment of infant outgrowth of cow’s milk allergy in relation to the faecal microbiome and metaproteome

Diana Hendrickx; Ran An; Sjef Boeren; Sumanth Mutte; Pantipa Chatchatee; Anna Nowak-Wegrzyn; Lars Lange; Suwat Benjaponpitak; Kok  Wee Chong; Pasuree Sangsupawanich; Marleen van Ampting; Manon Oude Nijhuis; Lucien Harthoorn; Jane Langford; Jan Knol; Karen Knipping; Johan Garssen; Valerie Trendelenburg; Robert Pesek; Carla Davis; Antonella Muraro; Mich Erlewyn-Lajeunesse; Adam Fox; Louise Michaelis; Kirsten Beyer; Lee Noimark; Gary Stiefel; Uwe Schauer; Eckard Hamelmann; Diego Peroni; Attilio Boner; Jolanda Lambert; Clara Belzer

doi:10.1038/s41598-023-39260-w

Assessment of infant outgrowth of cow’s milk allergy in relation to the faecal microbiome and metaproteome

Diana Hendrickx^*, Ran An, Sjef Boeren, Sumanth Mutte, Pantipa Chatchatee, Anna Nowak-Wegrzyn, Lars Lange, Suwat Benjaponpitak, Kok Wee Chong, Pasuree Sangsupawanich, Marleen van Ampting, Manon Oude Nijhuis, Lucien Harthoorn, Jane Langford, Jan Knol, Karen Knipping, Johan Garssen, Valerie Trendelenburg, Robert Pesek, Carla DavisAntonella Muraro, Mich Erlewyn-Lajeunesse, Adam Fox, Louise Michaelis, Kirsten Beyer, Lee Noimark, Gary Stiefel, Uwe Schauer, Eckard Hamelmann, Diego Peroni, Attilio Boner, Jolanda Lambert, Clara Belzer

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

21 Citations (Scopus)

Abstract

Previous studies provide evidence for an association between modifications of the gut microbiota in early life and the development of food allergies. We studied the faecal microbiota composition (16S rRNA gene amplicon sequencing) and faecal microbiome functionality (metaproteomics) in a cohort of 40 infants diagnosed with cow’s milk allergy (CMA) when entering the study. Some of the infants showed outgrowth of CMA after 12 months, while others did not. Faecal microbiota composition of infants was analysed directly after CMA diagnosis (baseline) as well as 6 and 12 months after entering the study. The aim was to gain insight on gut microbiome parameters in relation to outgrowth of CMA. The results of this study show that microbiome differences related to outgrowth of CMA can be mainly identified at the taxonomic level of the 16S rRNA gene, and to a lesser extent at the protein-based microbial taxonomy and functional protein level. At the 16S rRNA gene level outgrowth of CMA is characterized by lower relative abundance of Lachnospiraceae at baseline and lower Bacteroidaceae at visit 12 months.

Original language	English
Article number	12029
Journal	Scientific Reports
Volume	13
Issue number	1
DOIs	https://doi.org/10.1038/s41598-023-39260-w
Publication status	Published - 25 Jul 2023

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The role of nutrition in infant immune fitness
Hendrickx, D. (Creator), An, R. (Creator), Boeren, S. (Creator), Mutte, S. (Creator), Chatchatee, P. (Creator), Nowak-Wegrzyn, A. (Creator), Lange, L. (Creator), Benjaponpitak, S. (Creator), Wee Chong, K. (Creator), Sangsupawanich, P. (Creator), van Ampting, M. (Creator), Oude Nijhuis, M. (Creator), Harthoorn, L. (Creator), Langford, J. (Creator), Knol, J. (Creator), Knipping, K. (Creator), Garssen, J. (Creator), Trendelenburg, V. (Creator), Pesek, R. (Creator), Davis, C. (Creator), Muraro, A. (Creator), Erlewyn-Lajeunesse, M. (Creator), Fox, A. (Creator), Michaelis, L. (Creator), Beyer, K. (Creator), Noimark, L. (Creator), Stiefel, G. (Creator), Schauer, U. (Creator), Hamelmann, E. (Creator), Peroni, D. (Creator), Boner, A. (Creator), Lambert, J. (Creator) & Belzer, C. (Creator), Nutricia, 13 Jul 2023
https://www.ebi.ac.uk/ena/browser/view/PRJEB56782
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Hendrickx, D., An, R., Boeren, S., Mutte, S., Chatchatee, P., Nowak-Wegrzyn, A., Lange, L., Benjaponpitak, S., Wee Chong, K., Sangsupawanich, P., van Ampting, M., Oude Nijhuis, M., Harthoorn, L., Langford, J., Knol, J., Knipping, K., Garssen, J., Trendelenburg, V., Pesek, R., ... Belzer, C. (2023). Assessment of infant outgrowth of cow’s milk allergy in relation to the faecal microbiome and metaproteome. Scientific Reports, 13(1), Article 12029 . https://doi.org/10.1038/s41598-023-39260-w

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abstract = "Previous studies provide evidence for an association between modifications of the gut microbiota in early life and the development of food allergies. We studied the faecal microbiota composition (16S rRNA gene amplicon sequencing) and faecal microbiome functionality (metaproteomics) in a cohort of 40 infants diagnosed with cow{\textquoteright}s milk allergy (CMA) when entering the study. Some of the infants showed outgrowth of CMA after 12 months, while others did not. Faecal microbiota composition of infants was analysed directly after CMA diagnosis (baseline) as well as 6 and 12 months after entering the study. The aim was to gain insight on gut microbiome parameters in relation to outgrowth of CMA. The results of this study show that microbiome differences related to outgrowth of CMA can be mainly identified at the taxonomic level of the 16S rRNA gene, and to a lesser extent at the protein-based microbial taxonomy and functional protein level. At the 16S rRNA gene level outgrowth of CMA is characterized by lower relative abundance of Lachnospiraceae at baseline and lower Bacteroidaceae at visit 12 months.",

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Hendrickx, D, An, R, Boeren, S, Mutte, S, Chatchatee, P, Nowak-Wegrzyn, A, Lange, L, Benjaponpitak, S, Wee Chong, K, Sangsupawanich, P, van Ampting, M, Oude Nijhuis, M, Harthoorn, L, Langford, J, Knol, J, Knipping, K, Garssen, J, Trendelenburg, V, Pesek, R, Davis, C, Muraro, A, Erlewyn-Lajeunesse, M, Fox, A, Michaelis, L, Beyer, K, Noimark, L, Stiefel, G, Schauer, U, Hamelmann, E, Peroni, D, Boner, A, Lambert, J & Belzer, C 2023, 'Assessment of infant outgrowth of cow’s milk allergy in relation to the faecal microbiome and metaproteome', Scientific Reports, vol. 13, no. 1, 12029 . https://doi.org/10.1038/s41598-023-39260-w

TY - JOUR

T1 - Assessment of infant outgrowth of cow’s milk allergy in relation to the faecal microbiome and metaproteome

AU - Hendrickx, Diana

AU - An, Ran

AU - Boeren, Sjef

AU - Mutte, Sumanth

AU - Chatchatee, Pantipa

AU - Nowak-Wegrzyn, Anna

AU - Lange, Lars

AU - Benjaponpitak, Suwat

AU - Wee Chong, Kok

AU - Sangsupawanich, Pasuree

AU - van Ampting, Marleen

AU - Oude Nijhuis, Manon

AU - Harthoorn, Lucien

AU - Langford, Jane

AU - Knol, Jan

AU - Knipping, Karen

AU - Garssen, Johan

AU - Trendelenburg, Valerie

AU - Pesek, Robert

AU - Davis, Carla

AU - Muraro, Antonella

AU - Erlewyn-Lajeunesse, Mich

AU - Fox, Adam

AU - Michaelis, Louise

AU - Beyer, Kirsten

AU - Noimark, Lee

AU - Stiefel, Gary

AU - Schauer, Uwe

AU - Hamelmann, Eckard

AU - Peroni, Diego

AU - Boner, Attilio

AU - Lambert, Jolanda

AU - Belzer, Clara

PY - 2023/7/25

Y1 - 2023/7/25

N2 - Previous studies provide evidence for an association between modifications of the gut microbiota in early life and the development of food allergies. We studied the faecal microbiota composition (16S rRNA gene amplicon sequencing) and faecal microbiome functionality (metaproteomics) in a cohort of 40 infants diagnosed with cow’s milk allergy (CMA) when entering the study. Some of the infants showed outgrowth of CMA after 12 months, while others did not. Faecal microbiota composition of infants was analysed directly after CMA diagnosis (baseline) as well as 6 and 12 months after entering the study. The aim was to gain insight on gut microbiome parameters in relation to outgrowth of CMA. The results of this study show that microbiome differences related to outgrowth of CMA can be mainly identified at the taxonomic level of the 16S rRNA gene, and to a lesser extent at the protein-based microbial taxonomy and functional protein level. At the 16S rRNA gene level outgrowth of CMA is characterized by lower relative abundance of Lachnospiraceae at baseline and lower Bacteroidaceae at visit 12 months.

AB - Previous studies provide evidence for an association between modifications of the gut microbiota in early life and the development of food allergies. We studied the faecal microbiota composition (16S rRNA gene amplicon sequencing) and faecal microbiome functionality (metaproteomics) in a cohort of 40 infants diagnosed with cow’s milk allergy (CMA) when entering the study. Some of the infants showed outgrowth of CMA after 12 months, while others did not. Faecal microbiota composition of infants was analysed directly after CMA diagnosis (baseline) as well as 6 and 12 months after entering the study. The aim was to gain insight on gut microbiome parameters in relation to outgrowth of CMA. The results of this study show that microbiome differences related to outgrowth of CMA can be mainly identified at the taxonomic level of the 16S rRNA gene, and to a lesser extent at the protein-based microbial taxonomy and functional protein level. At the 16S rRNA gene level outgrowth of CMA is characterized by lower relative abundance of Lachnospiraceae at baseline and lower Bacteroidaceae at visit 12 months.

U2 - 10.1038/s41598-023-39260-w

DO - 10.1038/s41598-023-39260-w

M3 - Article

SN - 2045-2322

VL - 13

JO - Scientific Reports

JF - Scientific Reports

IS - 1

M1 - 12029

ER -

Assessment of infant outgrowth of cow’s milk allergy in relation to the faecal microbiome and metaproteome

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The role of nutrition in infant immune fitness

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