Assessment of compatible solutes to overcome salinity stress in thermophilic (55 oC) methanol-fed sulfate reducing granular sludges

M.V.G. Vallero, G. Lettinga, P.N.L. Lens

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

High NaCl concentrations (25 g.L-1) considerably decreased the methanol depletion rates for sludges harvested from two lab-scale sulfate reducing UASB reactors. In addition, 25 gNaCl.L-1 strongly affected the fate of methanol degradation, with clear increase in the acetate production at the expense of sulfide and methane production. The addition of different osmoprotectants, viz. glutamate, betaine, ectoine, choline, a mixture of compatible solutes and K+ and Mg2+, slightly increased methanol depletion rates for UASB reactors sludges. However, the acceleration in the methanol uptake rate favored the homoacetogenic bacteria, as the methanol breakdown was steered to the formation of acetate without increasing sulfate reduction and methane production rates. Thus, the compatible solutes used in this work were not effective as osmoprotectants to alleviate the acute NaCl toxicity on sulfate reducing granular sludges developed in methanol degrading thermophilic (55°C) UASB reactors
High NaCl concentrations (25 g(.)L(-1)) considerably decreased the methanol depletion rates for sludges harvested from two lab-scale sulfate reducing UASB reactors. In addition, 25 gNaCl.L-1 strongly affected the fate of methanol degradation, with clear increase in the acetate production at the expense of sulfide and,methane production. The addition of different osmoprotectants, viz. glutmate, betaine, ectoine, choline, a mixture of compatible solutes and K+ and Mg2+, slightly increased methanol depletion rates for UASB reactors sludges. However, the acceleration in the methanol uptake rate favored the homoacetogenic bacteria, as the methanol breakdown was steered to the formation of acetate without increasing sulfate reduction and methane production rates. Thus, the compatible solutes used in this work were not effective as osmoprotectants to alleviate the acute NaCl toxicity on sulfate reducing granular sludges developed in methanol degrading thermophilic (55degreesC) UASB reactors.
Original languageEnglish
Pages (from-to)195-202
JournalWater Science and Technology
Volume48
Issue number6
DOIs
Publication statusPublished - 2003

Fingerprint

methanol
solute
Methanol
sludge
sulfate
salinity
acetate
Methane
methane
Betaines
Toxicity
Sulfates
Bacteria
sulfide
toxicity
Degradation
degradation
bacterium
rate
reactor

Keywords

  • anaerobic treatment
  • waste water treatment
  • methanol
  • reduction
  • sodium chloride
  • sulfates
  • sewage effluent
  • sludges
  • glycine betaine
  • growing-cells
  • accumulation
  • degradation
  • adaptation
  • trehalose
  • glutamate
  • reactor

Cite this

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title = "Assessment of compatible solutes to overcome salinity stress in thermophilic (55 oC) methanol-fed sulfate reducing granular sludges",
abstract = "High NaCl concentrations (25 g.L-1) considerably decreased the methanol depletion rates for sludges harvested from two lab-scale sulfate reducing UASB reactors. In addition, 25 gNaCl.L-1 strongly affected the fate of methanol degradation, with clear increase in the acetate production at the expense of sulfide and methane production. The addition of different osmoprotectants, viz. glutamate, betaine, ectoine, choline, a mixture of compatible solutes and K+ and Mg2+, slightly increased methanol depletion rates for UASB reactors sludges. However, the acceleration in the methanol uptake rate favored the homoacetogenic bacteria, as the methanol breakdown was steered to the formation of acetate without increasing sulfate reduction and methane production rates. Thus, the compatible solutes used in this work were not effective as osmoprotectants to alleviate the acute NaCl toxicity on sulfate reducing granular sludges developed in methanol degrading thermophilic (55°C) UASB reactorsHigh NaCl concentrations (25 g(.)L(-1)) considerably decreased the methanol depletion rates for sludges harvested from two lab-scale sulfate reducing UASB reactors. In addition, 25 gNaCl.L-1 strongly affected the fate of methanol degradation, with clear increase in the acetate production at the expense of sulfide and,methane production. The addition of different osmoprotectants, viz. glutmate, betaine, ectoine, choline, a mixture of compatible solutes and K+ and Mg2+, slightly increased methanol depletion rates for UASB reactors sludges. However, the acceleration in the methanol uptake rate favored the homoacetogenic bacteria, as the methanol breakdown was steered to the formation of acetate without increasing sulfate reduction and methane production rates. Thus, the compatible solutes used in this work were not effective as osmoprotectants to alleviate the acute NaCl toxicity on sulfate reducing granular sludges developed in methanol degrading thermophilic (55degreesC) UASB reactors.",
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author = "M.V.G. Vallero and G. Lettinga and P.N.L. Lens",
year = "2003",
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language = "English",
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Assessment of compatible solutes to overcome salinity stress in thermophilic (55 oC) methanol-fed sulfate reducing granular sludges. / Vallero, M.V.G.; Lettinga, G.; Lens, P.N.L.

In: Water Science and Technology, Vol. 48, No. 6, 2003, p. 195-202.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Assessment of compatible solutes to overcome salinity stress in thermophilic (55 oC) methanol-fed sulfate reducing granular sludges

AU - Vallero, M.V.G.

AU - Lettinga, G.

AU - Lens, P.N.L.

PY - 2003

Y1 - 2003

N2 - High NaCl concentrations (25 g.L-1) considerably decreased the methanol depletion rates for sludges harvested from two lab-scale sulfate reducing UASB reactors. In addition, 25 gNaCl.L-1 strongly affected the fate of methanol degradation, with clear increase in the acetate production at the expense of sulfide and methane production. The addition of different osmoprotectants, viz. glutamate, betaine, ectoine, choline, a mixture of compatible solutes and K+ and Mg2+, slightly increased methanol depletion rates for UASB reactors sludges. However, the acceleration in the methanol uptake rate favored the homoacetogenic bacteria, as the methanol breakdown was steered to the formation of acetate without increasing sulfate reduction and methane production rates. Thus, the compatible solutes used in this work were not effective as osmoprotectants to alleviate the acute NaCl toxicity on sulfate reducing granular sludges developed in methanol degrading thermophilic (55°C) UASB reactorsHigh NaCl concentrations (25 g(.)L(-1)) considerably decreased the methanol depletion rates for sludges harvested from two lab-scale sulfate reducing UASB reactors. In addition, 25 gNaCl.L-1 strongly affected the fate of methanol degradation, with clear increase in the acetate production at the expense of sulfide and,methane production. The addition of different osmoprotectants, viz. glutmate, betaine, ectoine, choline, a mixture of compatible solutes and K+ and Mg2+, slightly increased methanol depletion rates for UASB reactors sludges. However, the acceleration in the methanol uptake rate favored the homoacetogenic bacteria, as the methanol breakdown was steered to the formation of acetate without increasing sulfate reduction and methane production rates. Thus, the compatible solutes used in this work were not effective as osmoprotectants to alleviate the acute NaCl toxicity on sulfate reducing granular sludges developed in methanol degrading thermophilic (55degreesC) UASB reactors.

AB - High NaCl concentrations (25 g.L-1) considerably decreased the methanol depletion rates for sludges harvested from two lab-scale sulfate reducing UASB reactors. In addition, 25 gNaCl.L-1 strongly affected the fate of methanol degradation, with clear increase in the acetate production at the expense of sulfide and methane production. The addition of different osmoprotectants, viz. glutamate, betaine, ectoine, choline, a mixture of compatible solutes and K+ and Mg2+, slightly increased methanol depletion rates for UASB reactors sludges. However, the acceleration in the methanol uptake rate favored the homoacetogenic bacteria, as the methanol breakdown was steered to the formation of acetate without increasing sulfate reduction and methane production rates. Thus, the compatible solutes used in this work were not effective as osmoprotectants to alleviate the acute NaCl toxicity on sulfate reducing granular sludges developed in methanol degrading thermophilic (55°C) UASB reactorsHigh NaCl concentrations (25 g(.)L(-1)) considerably decreased the methanol depletion rates for sludges harvested from two lab-scale sulfate reducing UASB reactors. In addition, 25 gNaCl.L-1 strongly affected the fate of methanol degradation, with clear increase in the acetate production at the expense of sulfide and,methane production. The addition of different osmoprotectants, viz. glutmate, betaine, ectoine, choline, a mixture of compatible solutes and K+ and Mg2+, slightly increased methanol depletion rates for UASB reactors sludges. However, the acceleration in the methanol uptake rate favored the homoacetogenic bacteria, as the methanol breakdown was steered to the formation of acetate without increasing sulfate reduction and methane production rates. Thus, the compatible solutes used in this work were not effective as osmoprotectants to alleviate the acute NaCl toxicity on sulfate reducing granular sludges developed in methanol degrading thermophilic (55degreesC) UASB reactors.

KW - anaërobe behandeling

KW - afvalwaterbehandeling

KW - methanol

KW - reductie

KW - natriumchloride

KW - sulfaten

KW - rioolafvalwater

KW - slib

KW - anaerobic treatment

KW - waste water treatment

KW - methanol

KW - reduction

KW - sodium chloride

KW - sulfates

KW - sewage effluent

KW - sludges

KW - glycine betaine

KW - growing-cells

KW - accumulation

KW - degradation

KW - adaptation

KW - trehalose

KW - glutamate

KW - reactor

U2 - 10.2166/wst.2003.0396

DO - 10.2166/wst.2003.0396

M3 - Article

VL - 48

SP - 195

EP - 202

JO - Water Science and Technology

JF - Water Science and Technology

SN - 0273-1223

IS - 6

ER -