Assessing the immunomodulatory potential of high-molecular-weight extracts from mushrooms; an assay based on THP-1 macrophages

J. van de Velde, R.H.P. Wilbers, L.B. Westerhof, D.R. van Raaij, I. Stavrakaki, A.S.M. Sonnenberg, J. Bakker, A. Schots

Research output: Contribution to journalArticleAcademicpeer-review

2 Citations (Scopus)

Abstract

BACKGROUND Food is a potential source of immunomodulating compounds that may be used to steer immune responses towards a desired status such as reducing inflammatory disorders. However, to identify and characterize such bioactive compounds, biologically relevant and standardized assays are required. Macrophages play an important role in immunomodulation and are suited for developing cell-based assays. An assay was developed based on macrophages, in a homogeneous differentiation state, using the human monocytic cell line THP-1 previously used to assess immunomodulatory properties of low-molecular-weight allergens, hormones, dietary supplements and therapeutic drugs. RESULTS Zymosan and mushroom polysaccharide extracts lead to a heterogeneous differentiation state of THP-1 monocytes, and these cells secrete low levels of cytokines upon stimulation. Differentiation into macrophages using a low concentration of phorbol 12-myristate 13-acetate improved responsiveness. Elevated levels of cytokines were secreted by cells in a homogenous differentiation state. In addition, it was determined that the assay performs best when using cells at a concentration of (2.5–5)¿×¿105 cells mL-1. CONCLUSION An assay was developed suitable to distinguish the immunomodulatory properties of food compounds in a reproducible manner. It was evaluated using eight mushroom species by measuring the secretion of relevant cytokines TNF-a, IL-1ß, IL-6 and IL-10. © 2014 Society of Chemical Industry
Original languageEnglish
Pages (from-to)344-350
JournalJournal of the Science of Food and Agriculture
Volume95
Issue number2
DOIs
Publication statusPublished - 2015

Keywords

  • monocytic leukemia-cells
  • agaricus-blazei murill
  • toll-like receptors
  • in-vitro
  • induction
  • responses
  • line
  • polysaccharides
  • differentiation
  • expression

Fingerprint Dive into the research topics of 'Assessing the immunomodulatory potential of high-molecular-weight extracts from mushrooms; an assay based on THP-1 macrophages'. Together they form a unique fingerprint.

  • Cite this