Aspergillus species from Brazilian dry beans and their toxigenic potential

Bárbara Alves dos Santos-Ciscon, Anne van Diepeningen, José da Cruz Machado, Iara Eleutéria Dias, Cees Waalwijk

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Aspergilli are common contaminants of food and feed and a major source of mycotoxins. In this study, 87 Aspergillus strains were isolated from beans from 14 different cities in Brazil and identified to the species level based on partial calmodulin and β-tubulin sequence data. All green spored isolates belonged to section Flavi and were identified as A. flavus (n = 39) or A. pseudocaelatus (n = 1). All black spored isolates belonged to section Nigri and were identified as A. niger (n = 24) or A. luchuensis (n = 10), while the yellow spored strains were identified as A. westerdijkiae (n = 7), A. ostianus (n = 3), A. ochraceus (n = 1) or A. wentii (n = 2). The toxigenic potential of these Aspergillus strains from beans was studied by the prospection of genes in three of the major mycotoxin clusters: aflatoxin (seven genes checked), ochratoxin A (four genes) and fumonisin (ten genes and two intergenic regions). Genes involved in the biosynthesis of aflatoxin were only detected in A. flavus isolates: 17/39 A. flavus isolates proved to contain all the aflatoxin genes tested, the others missed one or more genes. The full complement of fumonisin biosynthesis genes was identified in all A. niger isolates. Finally, no genes for ochratoxin A were detected in any of the isolates. Our work suggests that aflatoxin production by some A. flavus strains and fumonisin production by A. niger isolates form the largest mycotoxin risks in Brazilian beans.

LanguageEnglish
Pages91-100
JournalInternational Journal of Food Microbiology
Volume292
DOIs
Publication statusPublished - 2 Mar 2019

Fingerprint

dry beans
Aspergillus
Aflatoxins
Genes
Fumonisins
aflatoxins
genes
Mycotoxins
fumonisins
mycotoxins
beans
ochratoxin A
biosynthesis
feed contamination
Intergenic DNA
food contamination
calmodulin
Calmodulin
Tubulin
tubulin

Keywords

  • A. flavus
  • A. luchuensis
  • A. niger
  • Aflatoxin
  • Fumonisin
  • Ochratoxin A

Cite this

dos Santos-Ciscon, Bárbara Alves ; van Diepeningen, Anne ; da Cruz Machado, José ; Dias, Iara Eleutéria ; Waalwijk, Cees. / Aspergillus species from Brazilian dry beans and their toxigenic potential. In: International Journal of Food Microbiology. 2019 ; Vol. 292. pp. 91-100.
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Aspergillus species from Brazilian dry beans and their toxigenic potential. / dos Santos-Ciscon, Bárbara Alves; van Diepeningen, Anne; da Cruz Machado, José; Dias, Iara Eleutéria; Waalwijk, Cees.

In: International Journal of Food Microbiology, Vol. 292, 02.03.2019, p. 91-100.

Research output: Contribution to journalArticleAcademicpeer-review

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