Archaeal actin-family filament systems

Ann Christin Lindås*, Karin Valegård, Thijs J.G. Ettema

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Citations (Scopus)

Abstract

Actin represents one of the most abundant and conserved eukaryotic proteins over time, and has an important role in many different cellular processes such as cell shape determination, motility, force generation, cytokinesis, amongst many others. Eukaryotic actin has been studied for decades and was for a long time considered a eukaryote-specific trait. However, in the early 2000s a bacterial actin homolog, MreB, was identified, characterized and found to have a cytoskeletal function and group within the superfamily of actin proteins. More recently, an actin cytoskeleton was also identified in archaea. The genome of the hyperthermophilic crenarchaeon Pyrobaculum calidifontis contains a five-gene cluster named Arcade encoding for an actin homolog, Crenactin, polymerizing into helical filaments spanning the whole length of the cell. Phylogenetic and structural studies place Crenactin closer to the eukaryotic actin than to the bacterial homologues. A significant difference, however, is that Crenactin can form single helical filaments in addition to filaments containing two intertwined proto filaments. The genome of the recently discovered Lokiarchaeota encodes several different actin homologues, termed Lokiactins, which are even more closely related to the eukaryotic actin than Crenactin. A primitive, dynamic actin-based cytoskeleton in archaea could have enabled the engulfment of the alphaproteobacterial progenitor of the mitochondria, a key-event in the evolution of eukaryotes.

Original languageEnglish
Title of host publicationProkaryotic Cytoskeletons
Subtitle of host publicationFilamentous Protein Polymers Active in the Cytoplasm of Bacterial and Archaeal Cells
EditorsJan Löwe, Linda A. Amos
Place of PublicationCham
PublisherSpringer
Chapter13
Pages379-392
Number of pages14
ISBN (Electronic)9783319530475
ISBN (Print)9783319530451
DOIs
Publication statusPublished - 2017
Externally publishedYes

Publication series

NameSub-Cellular Biochemistry
PublisherWiley
Volume84
ISSN (Print)0306-0225

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Keywords

  • Actin superfamily in archaea
  • Archaeal cytoskeleton
  • Crenactin
  • Crenarchaea
  • Helical filaments
  • MreB - Arcadin
  • Pyrobaculum calidifontis

Cite this

Lindås, A. C., Valegård, K., & Ettema, T. J. G. (2017). Archaeal actin-family filament systems. In J. Löwe, & L. A. Amos (Eds.), Prokaryotic Cytoskeletons: Filamentous Protein Polymers Active in the Cytoplasm of Bacterial and Archaeal Cells (pp. 379-392). (Sub-Cellular Biochemistry; Vol. 84). Cham: Springer. https://doi.org/10.1007/978-3-319-53047-5_13