Arabidopsis semidwarfs evolved from independent mutations in GA20ox1, ortholog to green revolution dwarf alleles in rice and barley

L. Barboza, S. Effgen, C. Alonso-Blanco, R. Kooke, J.J.B. Keurentjes, M. Koornneef

Research output: Contribution to journalArticleAcademicpeer-review

50 Citations (Scopus)

Abstract

Understanding the genetic bases of natural variation for developmental and stress-related traits is a major goal of current plant biology. Variation in plant hormone levels and signaling might underlie such phenotypic variation occurring even within the same species. Here we report the genetic and molecular basis of semidwarf individuals found in natural Arabidopsis thaliana populations. Allelism tests demonstrate that independent loss-offunction mutations at GA locus 5 (GA5), which encodes gibberellin 20-oxidase 1 (GA20ox1) involved in the last steps of gibberellin biosynthesis, are found in different populations from southern, western, and northern Europe; central Asia; and Japan. Sequencing of GA5 identified 21 different loss-of-function alleles causing semidwarfness without any obvious general tradeoff affecting plant performance traits. GA5 shows signatures of purifying selection, whereas GA5 loss-of-function alleles can also exhibit patterns of positive selection in specific populations as shown by Fay and Wu’s H statistics. These results suggest that antagonistic pleiotropy might underlie the occurrence of GA5 loss-of-function mutations in nature. Furthermore, because GA5 is the ortholog of rice SD1 and barley Sdw1/Denso green revolution genes, this study illustrates the occurrence of conserved adaptive evolution between wild A.thaliana and domesticated plants
Original languageEnglish
Pages (from-to)15818-15823
JournalProceedings of the National Academy of Sciences of the United States of America
Volume110
Issue number39
DOIs
Publication statusPublished - 2013

Keywords

  • quantitative trait loci
  • gibberellin biosynthesis
  • natural variation
  • thaliana
  • gene
  • populations
  • model
  • polymorphism
  • association
  • adaptation

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