Arabidopsis myrosinases link the glucosinolate-myrosinase system and the cuticle

Ishita Ahuja*, Ric C.H. de Vos, Jens Rohloff, Geert M. Stoopen, Kari K. Halle, Samina Jam Nazeer Ahmad, Linh Hoang, Robert D. Hall, Atle M. Bones

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

10 Citations (Scopus)

Abstract

Both physical barriers and reactive phytochemicals represent two important components of a plant's defence system against environmental stress. However, these two defence systems have generally been studied independently. Here, we have taken an exclusive opportunity to investigate the connection between a chemical-based plant defence system, represented by the glucosinolate-myrosinase system, and a physical barrier, represented by the cuticle, using Arabidopsis myrosinase (thioglucosidase; TGG) mutants. The tgg1, single and tgg1 tgg2 double mutants showed morphological changes compared to wild-type plants visible as changes in pavement cells, stomatal cells and the ultrastructure of the cuticle. Extensive metabolite analyses of leaves from tgg mutants and wild-type Arabidopsis plants showed altered levels of cuticular fatty acids, fatty acid phytyl esters, glucosinolates, and indole compounds in tgg single and double mutants as compared to wild-type plants. These results point to a close and novel association between chemical defence systems and physical defence barriers.

Original languageEnglish
Article number38990
JournalScientific Reports
Volume6
DOIs
Publication statusPublished - 2016

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