Arabidopsis myrosinases link the glucosinolate-myrosinase system and the cuticle

Ishita Ahuja*, Ric C.H. de Vos, Jens Rohloff, Geert M. Stoopen, Kari K. Halle, Samina Jam Nazeer Ahmad, Linh Hoang, Robert D. Hall, Atle M. Bones

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

8 Citations (Scopus)

Abstract

Both physical barriers and reactive phytochemicals represent two important components of a plant's defence system against environmental stress. However, these two defence systems have generally been studied independently. Here, we have taken an exclusive opportunity to investigate the connection between a chemical-based plant defence system, represented by the glucosinolate-myrosinase system, and a physical barrier, represented by the cuticle, using Arabidopsis myrosinase (thioglucosidase; TGG) mutants. The tgg1, single and tgg1 tgg2 double mutants showed morphological changes compared to wild-type plants visible as changes in pavement cells, stomatal cells and the ultrastructure of the cuticle. Extensive metabolite analyses of leaves from tgg mutants and wild-type Arabidopsis plants showed altered levels of cuticular fatty acids, fatty acid phytyl esters, glucosinolates, and indole compounds in tgg single and double mutants as compared to wild-type plants. These results point to a close and novel association between chemical defence systems and physical defence barriers.

Original languageEnglish
Article number38990
JournalScientific Reports
Volume6
DOIs
Publication statusPublished - 2016

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thioglucosidase
glucosinolates
Arabidopsis
mutants
fatty acid esters
indoles
phytopharmaceuticals
ultrastructure
cells
metabolites
fatty acids
leaves

Cite this

Ahuja, Ishita ; de Vos, Ric C.H. ; Rohloff, Jens ; Stoopen, Geert M. ; Halle, Kari K. ; Ahmad, Samina Jam Nazeer ; Hoang, Linh ; Hall, Robert D. ; Bones, Atle M. / Arabidopsis myrosinases link the glucosinolate-myrosinase system and the cuticle. In: Scientific Reports. 2016 ; Vol. 6.
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title = "Arabidopsis myrosinases link the glucosinolate-myrosinase system and the cuticle",
abstract = "Both physical barriers and reactive phytochemicals represent two important components of a plant's defence system against environmental stress. However, these two defence systems have generally been studied independently. Here, we have taken an exclusive opportunity to investigate the connection between a chemical-based plant defence system, represented by the glucosinolate-myrosinase system, and a physical barrier, represented by the cuticle, using Arabidopsis myrosinase (thioglucosidase; TGG) mutants. The tgg1, single and tgg1 tgg2 double mutants showed morphological changes compared to wild-type plants visible as changes in pavement cells, stomatal cells and the ultrastructure of the cuticle. Extensive metabolite analyses of leaves from tgg mutants and wild-type Arabidopsis plants showed altered levels of cuticular fatty acids, fatty acid phytyl esters, glucosinolates, and indole compounds in tgg single and double mutants as compared to wild-type plants. These results point to a close and novel association between chemical defence systems and physical defence barriers.",
author = "Ishita Ahuja and {de Vos}, {Ric C.H.} and Jens Rohloff and Stoopen, {Geert M.} and Halle, {Kari K.} and Ahmad, {Samina Jam Nazeer} and Linh Hoang and Hall, {Robert D.} and Bones, {Atle M.}",
year = "2016",
doi = "10.1038/srep38990",
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volume = "6",
journal = "Scientific Reports",
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Arabidopsis myrosinases link the glucosinolate-myrosinase system and the cuticle. / Ahuja, Ishita; de Vos, Ric C.H.; Rohloff, Jens; Stoopen, Geert M.; Halle, Kari K.; Ahmad, Samina Jam Nazeer; Hoang, Linh; Hall, Robert D.; Bones, Atle M.

In: Scientific Reports, Vol. 6, 38990, 2016.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Arabidopsis myrosinases link the glucosinolate-myrosinase system and the cuticle

AU - Ahuja, Ishita

AU - de Vos, Ric C.H.

AU - Rohloff, Jens

AU - Stoopen, Geert M.

AU - Halle, Kari K.

AU - Ahmad, Samina Jam Nazeer

AU - Hoang, Linh

AU - Hall, Robert D.

AU - Bones, Atle M.

PY - 2016

Y1 - 2016

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AB - Both physical barriers and reactive phytochemicals represent two important components of a plant's defence system against environmental stress. However, these two defence systems have generally been studied independently. Here, we have taken an exclusive opportunity to investigate the connection between a chemical-based plant defence system, represented by the glucosinolate-myrosinase system, and a physical barrier, represented by the cuticle, using Arabidopsis myrosinase (thioglucosidase; TGG) mutants. The tgg1, single and tgg1 tgg2 double mutants showed morphological changes compared to wild-type plants visible as changes in pavement cells, stomatal cells and the ultrastructure of the cuticle. Extensive metabolite analyses of leaves from tgg mutants and wild-type Arabidopsis plants showed altered levels of cuticular fatty acids, fatty acid phytyl esters, glucosinolates, and indole compounds in tgg single and double mutants as compared to wild-type plants. These results point to a close and novel association between chemical defence systems and physical defence barriers.

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