Arabidopsis miRNA boosts host immunity against Verticillium dahliae via repression of plant target gene transcript

M.M.A. van Damme, G. van den Berg, L. Faino, B.P.H.J. Thomma

Research output: Contribution to conferenceAbstractAcademic

Abstract

RNA silencing is a eukaryotic mechanism that exploits small non-coding RNAs (small-RNAs) to regulate gene expression in a sequence-specific manner in many cellular processes, including innate immunity. The plant immune system is a known target of RNA silencing by several pathogens, e.g. viruses and bacteria. The fungus Verticillium dahliae is the causal agent of Verticillium wilt disease. Data from our laboratory indicate that the Arabidopsis thaliana RNA silencing machinery plays a critical role during Verticillium wilt disease (Ellendorff et al., 2009). By combining small-RNA and transcript profiling we identified small-RNAs and their reciprocal target transcripts in V. dahliae-infected A. thaliana plants. Accumulation of a particular A. thaliana miRNA during the interaction, and repression of its reciprocal targeted transcript, causes enhanced resistance to Verticillium wilt disease. We show that A. thaliana T-DNA insertion mutants that lack the target transcript display enhanced resistance to Verticillium wilt disease. Mature miRNAs are generated through multiple processing steps from primary transcripts (pri-miRNA or MIRNA gene) that contain imperfect foldback structures. We are currently analyzing if transgenic A. thaliana lines that overexpress the MIRNA gene also display enhanced resistance to Verticillium wilt disease.
Original languageEnglish
Publication statusPublished - 2016
EventXVII International Congress on Molecular Plant-Microbe Interactions - Portland, Oregon, United States
Duration: 17 Jul 201621 Jul 2016

Conference

ConferenceXVII International Congress on Molecular Plant-Microbe Interactions
CountryUnited States
CityPortland, Oregon
Period17/07/1621/07/16

Fingerprint

Verticillium wilt
Verticillium dahliae
microRNA
Arabidopsis thaliana
immunity
Arabidopsis
RNA interference
genes
immune system
genetically modified organisms
gene expression
viruses
mutants
fungi
pathogens
bacteria
DNA
non-coding RNA

Cite this

van Damme, M. M. A., van den Berg, G., Faino, L., & Thomma, B. P. H. J. (2016). Arabidopsis miRNA boosts host immunity against Verticillium dahliae via repression of plant target gene transcript. Abstract from XVII International Congress on Molecular Plant-Microbe Interactions, Portland, Oregon, United States.
van Damme, M.M.A. ; van den Berg, G. ; Faino, L. ; Thomma, B.P.H.J. / Arabidopsis miRNA boosts host immunity against Verticillium dahliae via repression of plant target gene transcript. Abstract from XVII International Congress on Molecular Plant-Microbe Interactions, Portland, Oregon, United States.
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abstract = "RNA silencing is a eukaryotic mechanism that exploits small non-coding RNAs (small-RNAs) to regulate gene expression in a sequence-specific manner in many cellular processes, including innate immunity. The plant immune system is a known target of RNA silencing by several pathogens, e.g. viruses and bacteria. The fungus Verticillium dahliae is the causal agent of Verticillium wilt disease. Data from our laboratory indicate that the Arabidopsis thaliana RNA silencing machinery plays a critical role during Verticillium wilt disease (Ellendorff et al., 2009). By combining small-RNA and transcript profiling we identified small-RNAs and their reciprocal target transcripts in V. dahliae-infected A. thaliana plants. Accumulation of a particular A. thaliana miRNA during the interaction, and repression of its reciprocal targeted transcript, causes enhanced resistance to Verticillium wilt disease. We show that A. thaliana T-DNA insertion mutants that lack the target transcript display enhanced resistance to Verticillium wilt disease. Mature miRNAs are generated through multiple processing steps from primary transcripts (pri-miRNA or MIRNA gene) that contain imperfect foldback structures. We are currently analyzing if transgenic A. thaliana lines that overexpress the MIRNA gene also display enhanced resistance to Verticillium wilt disease.",
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van Damme, MMA, van den Berg, G, Faino, L & Thomma, BPHJ 2016, 'Arabidopsis miRNA boosts host immunity against Verticillium dahliae via repression of plant target gene transcript' XVII International Congress on Molecular Plant-Microbe Interactions, Portland, Oregon, United States, 17/07/16 - 21/07/16, .

Arabidopsis miRNA boosts host immunity against Verticillium dahliae via repression of plant target gene transcript. / van Damme, M.M.A.; van den Berg, G.; Faino, L.; Thomma, B.P.H.J.

2016. Abstract from XVII International Congress on Molecular Plant-Microbe Interactions, Portland, Oregon, United States.

Research output: Contribution to conferenceAbstractAcademic

TY - CONF

T1 - Arabidopsis miRNA boosts host immunity against Verticillium dahliae via repression of plant target gene transcript

AU - van Damme, M.M.A.

AU - van den Berg, G.

AU - Faino, L.

AU - Thomma, B.P.H.J.

PY - 2016

Y1 - 2016

N2 - RNA silencing is a eukaryotic mechanism that exploits small non-coding RNAs (small-RNAs) to regulate gene expression in a sequence-specific manner in many cellular processes, including innate immunity. The plant immune system is a known target of RNA silencing by several pathogens, e.g. viruses and bacteria. The fungus Verticillium dahliae is the causal agent of Verticillium wilt disease. Data from our laboratory indicate that the Arabidopsis thaliana RNA silencing machinery plays a critical role during Verticillium wilt disease (Ellendorff et al., 2009). By combining small-RNA and transcript profiling we identified small-RNAs and their reciprocal target transcripts in V. dahliae-infected A. thaliana plants. Accumulation of a particular A. thaliana miRNA during the interaction, and repression of its reciprocal targeted transcript, causes enhanced resistance to Verticillium wilt disease. We show that A. thaliana T-DNA insertion mutants that lack the target transcript display enhanced resistance to Verticillium wilt disease. Mature miRNAs are generated through multiple processing steps from primary transcripts (pri-miRNA or MIRNA gene) that contain imperfect foldback structures. We are currently analyzing if transgenic A. thaliana lines that overexpress the MIRNA gene also display enhanced resistance to Verticillium wilt disease.

AB - RNA silencing is a eukaryotic mechanism that exploits small non-coding RNAs (small-RNAs) to regulate gene expression in a sequence-specific manner in many cellular processes, including innate immunity. The plant immune system is a known target of RNA silencing by several pathogens, e.g. viruses and bacteria. The fungus Verticillium dahliae is the causal agent of Verticillium wilt disease. Data from our laboratory indicate that the Arabidopsis thaliana RNA silencing machinery plays a critical role during Verticillium wilt disease (Ellendorff et al., 2009). By combining small-RNA and transcript profiling we identified small-RNAs and their reciprocal target transcripts in V. dahliae-infected A. thaliana plants. Accumulation of a particular A. thaliana miRNA during the interaction, and repression of its reciprocal targeted transcript, causes enhanced resistance to Verticillium wilt disease. We show that A. thaliana T-DNA insertion mutants that lack the target transcript display enhanced resistance to Verticillium wilt disease. Mature miRNAs are generated through multiple processing steps from primary transcripts (pri-miRNA or MIRNA gene) that contain imperfect foldback structures. We are currently analyzing if transgenic A. thaliana lines that overexpress the MIRNA gene also display enhanced resistance to Verticillium wilt disease.

M3 - Abstract

ER -

van Damme MMA, van den Berg G, Faino L, Thomma BPHJ. Arabidopsis miRNA boosts host immunity against Verticillium dahliae via repression of plant target gene transcript. 2016. Abstract from XVII International Congress on Molecular Plant-Microbe Interactions, Portland, Oregon, United States.