Arabidopsis latent virus 1, a comovirus widely spread in Arabidopsis thaliana collections

Ava Verhoeven, Karen J. Kloth, Anne Kupczok, Geert H. Oymans, Janna Damen, Karin Rijnsburger, Zhang Jiang, Cas Deelen, Rashmi Sasidharan, Martijn van Zanten, René A.A. van der Vlugt*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

1 Citation (Scopus)

Abstract

Transcriptome studies of Illumina RNA-Seq datasets of different Arabidopsis thaliana natural accessions and T-DNA mutants revealed the presence of two virus-like RNA sequences which showed the typical two-segmented genome characteristics of a comovirus. This comovirus did not induce any visible symptoms in infected A. thaliana plants cultivated under standard laboratory conditions. Hence it was named Arabidopsis latent virus 1 (ArLV1). Virus infectivity in A. thaliana plants was confirmed by quantitative reverse transcription polymerase chain reaction, transmission electron microscopy and mechanical inoculation. Arabidopsis latent virus 1 can also mechanically infect Nicotiana benthamiana, causing distinct mosaic symptoms. A bioinformatics investigation of A. thaliana RNA-Seq repositories, including nearly 6500 Sequence Read Archives (SRAs) in the NCBI SRA database, revealed the presence of ArLV1 in 25% of all archived natural A. thaliana accessions and in 8.5% of all analyzed SRAs. Arabidopsis latent virus 1 could also be detected in A. thaliana plants collected from the wild. Arabidopsis latent virus 1 is highly seed-transmissible with up to 40% incidence on the progeny derived from infected A. thaliana plants. This has probably led to a worldwide distribution in the model plant A. thaliana with as yet unknown effects on plant performance in a substantial number of studies.

Original languageEnglish
Pages (from-to)1146-1153
JournalNew Phytologist
Volume237
Issue number4
Early online date2022
DOIs
Publication statusPublished - Feb 2023

Keywords

  • Arabidopsis latent virus 1 (ArLV1)
  • Arabidopsis thaliana
  • comovirus
  • drought resilience
  • RNA sequencing
  • sequence read archives

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