Aptamer-based fluorometric determination of norovirus using a paper-based microfluidic device

Xuan Weng, Suresh Neethirajan*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

31 Citations (Scopus)

Abstract

The authors describe a rapid and highly sensitive point-of-care device for rapid determination of noroviruses, a leading cause of acute gastroenteritis. The assay is based on the use of a norovirus-specific aptamer labeled with 6-carboxyfluorescein, and of multi-walled carbon nanotubes (MWCNT) and graphene oxide (GO). The fluorescence of the 6-FAM labeled aptamer is quenched by MWCNT or GO. In the presence of norovirus, fluorescence is recovered due to the release of the labeled aptamer from MWCNT or GO. An easy-to-make paper-based microfluidic platform was developed using a nitrocellulose membrane. The quantitation of norovirus was successfully performed. The linear range extends from 13 ng·mL−1 to 13 μg·mL−1 of norovirus. The detection limits are 4.4 ng·mL−1 and 3.3 ng·mL−1, respectively, when using MWCNT or GO. The device is simple and cost-effective, and holds the potential of rapid in-situ visual determination of noroviruses with remarkable sensitivity and specificity. Hence, it provides a new method for early identification of norovirus and a tool for early intervention when preventing the spread of an outbreak. [Figure not available: see fulltext.].

Original languageEnglish
Pages (from-to)4545-4552
Number of pages8
JournalMicrochimica acta
Volume184
Issue number11
DOIs
Publication statusPublished - 11 Sep 2017
Externally publishedYes

Keywords

  • Aptamer
  • Biosensor
  • Graphene oxide
  • Multi-walled carbon nanotubes
  • Nitrocellulose membrane
  • Norovirus
  • Paper-based microfluidic device

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