Applying the adverse outcome pathway (AOP) for food sensitization to support in vitro testing strategies

Daniel Lozano-Ojalvo, Sara Benedé, Celia M. Antunes, Simona L. Bavaro, Grégory Bouchaud, Ana Costa, Sandra Denery-Papini, Araceli Díaz-Perales, María Garrido-Arandia, Marija Gavrovic-Jankulovic, Simone Hayen, Mónica Martínez-Blanco, Elena Molina, Linda Monaci, Raymond H.H. Pieters, Clelia Villemin, Harry J. Wichers, Barbara Wróblewska, Linette E.M. Willemsen, Erwin L. Roggen & 1 others Jolanda H.M. van Bilsen

Research output: Contribution to journalReview articleAcademicpeer-review

Abstract

Background: Before introducing proteins from new or alternative dietary sources into the market, a compressive risk assessment including food allergic sensitization should be carried out in order to ensure their safety. We have recently proposed the adverse outcome pathway (AOP) concept to structure the current mechanistic understanding of the molecular and cellular pathways evidenced to drive IgE-mediated food allergies. This AOP framework offers the biological context to collect and structure existing in vitro methods and to identify missing assays to evaluate sensitizing potential of food proteins. Scope and approach: In this review, we provide a state-of-the-art overview of available in vitro approaches for assessing the sensitizing potential of food proteins, including their strengths and limitations. These approaches are structured by their potential to evaluate the molecular initiating and key events driving food sensitization. Key findings and conclusions: The application of the AOP framework offers the opportunity to anchor existing testing methods to specific building blocks of the AOP for food sensitization. In general, in vitro methods evaluating mechanisms involved in the innate immune response are easier to address than assays addressing the adaptive immune response due to the low precursor frequency of allergen-specific T and B cells. Novel ex vivo culture strategies may have the potential to become useful tools for investigating the sensitizing potential of food proteins. When applied in the context of an integrated testing strategy, the described approaches may reduce, if not replace, current animal testing approaches.

Original languageEnglish
Pages (from-to)307-319
JournalTrends in Food Science and Technology
Volume85
DOIs
Publication statusPublished - 1 Mar 2019

Fingerprint

protein sources
Food
food allergies
testing
assays
allergens
risk assessment
B-lymphocytes
Proteins
T-lymphocytes
methodology
markets
Food Hypersensitivity
Adaptive Immunity
Innate Immunity
Allergens
Immunoglobulin E
In Vitro Techniques
animals
B-Lymphocytes

Keywords

  • Adverse outcome pathway
  • Dendritic cells
  • Epithelial cells
  • IgE-mediated food allergy
  • In vitro models
  • T and B cells

Cite this

Lozano-Ojalvo, D., Benedé, S., Antunes, C. M., Bavaro, S. L., Bouchaud, G., Costa, A., ... van Bilsen, J. H. M. (2019). Applying the adverse outcome pathway (AOP) for food sensitization to support in vitro testing strategies. Trends in Food Science and Technology, 85, 307-319. https://doi.org/10.1016/j.tifs.2019.01.014
Lozano-Ojalvo, Daniel ; Benedé, Sara ; Antunes, Celia M. ; Bavaro, Simona L. ; Bouchaud, Grégory ; Costa, Ana ; Denery-Papini, Sandra ; Díaz-Perales, Araceli ; Garrido-Arandia, María ; Gavrovic-Jankulovic, Marija ; Hayen, Simone ; Martínez-Blanco, Mónica ; Molina, Elena ; Monaci, Linda ; Pieters, Raymond H.H. ; Villemin, Clelia ; Wichers, Harry J. ; Wróblewska, Barbara ; Willemsen, Linette E.M. ; Roggen, Erwin L. ; van Bilsen, Jolanda H.M. / Applying the adverse outcome pathway (AOP) for food sensitization to support in vitro testing strategies. In: Trends in Food Science and Technology. 2019 ; Vol. 85. pp. 307-319.
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abstract = "Background: Before introducing proteins from new or alternative dietary sources into the market, a compressive risk assessment including food allergic sensitization should be carried out in order to ensure their safety. We have recently proposed the adverse outcome pathway (AOP) concept to structure the current mechanistic understanding of the molecular and cellular pathways evidenced to drive IgE-mediated food allergies. This AOP framework offers the biological context to collect and structure existing in vitro methods and to identify missing assays to evaluate sensitizing potential of food proteins. Scope and approach: In this review, we provide a state-of-the-art overview of available in vitro approaches for assessing the sensitizing potential of food proteins, including their strengths and limitations. These approaches are structured by their potential to evaluate the molecular initiating and key events driving food sensitization. Key findings and conclusions: The application of the AOP framework offers the opportunity to anchor existing testing methods to specific building blocks of the AOP for food sensitization. In general, in vitro methods evaluating mechanisms involved in the innate immune response are easier to address than assays addressing the adaptive immune response due to the low precursor frequency of allergen-specific T and B cells. Novel ex vivo culture strategies may have the potential to become useful tools for investigating the sensitizing potential of food proteins. When applied in the context of an integrated testing strategy, the described approaches may reduce, if not replace, current animal testing approaches.",
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Lozano-Ojalvo, D, Benedé, S, Antunes, CM, Bavaro, SL, Bouchaud, G, Costa, A, Denery-Papini, S, Díaz-Perales, A, Garrido-Arandia, M, Gavrovic-Jankulovic, M, Hayen, S, Martínez-Blanco, M, Molina, E, Monaci, L, Pieters, RHH, Villemin, C, Wichers, HJ, Wróblewska, B, Willemsen, LEM, Roggen, EL & van Bilsen, JHM 2019, 'Applying the adverse outcome pathway (AOP) for food sensitization to support in vitro testing strategies' Trends in Food Science and Technology, vol. 85, pp. 307-319. https://doi.org/10.1016/j.tifs.2019.01.014

Applying the adverse outcome pathway (AOP) for food sensitization to support in vitro testing strategies. / Lozano-Ojalvo, Daniel; Benedé, Sara; Antunes, Celia M.; Bavaro, Simona L.; Bouchaud, Grégory; Costa, Ana; Denery-Papini, Sandra; Díaz-Perales, Araceli; Garrido-Arandia, María; Gavrovic-Jankulovic, Marija; Hayen, Simone; Martínez-Blanco, Mónica; Molina, Elena; Monaci, Linda; Pieters, Raymond H.H.; Villemin, Clelia; Wichers, Harry J.; Wróblewska, Barbara; Willemsen, Linette E.M.; Roggen, Erwin L.; van Bilsen, Jolanda H.M.

In: Trends in Food Science and Technology, Vol. 85, 01.03.2019, p. 307-319.

Research output: Contribution to journalReview articleAcademicpeer-review

TY - JOUR

T1 - Applying the adverse outcome pathway (AOP) for food sensitization to support in vitro testing strategies

AU - Lozano-Ojalvo, Daniel

AU - Benedé, Sara

AU - Antunes, Celia M.

AU - Bavaro, Simona L.

AU - Bouchaud, Grégory

AU - Costa, Ana

AU - Denery-Papini, Sandra

AU - Díaz-Perales, Araceli

AU - Garrido-Arandia, María

AU - Gavrovic-Jankulovic, Marija

AU - Hayen, Simone

AU - Martínez-Blanco, Mónica

AU - Molina, Elena

AU - Monaci, Linda

AU - Pieters, Raymond H.H.

AU - Villemin, Clelia

AU - Wichers, Harry J.

AU - Wróblewska, Barbara

AU - Willemsen, Linette E.M.

AU - Roggen, Erwin L.

AU - van Bilsen, Jolanda H.M.

PY - 2019/3/1

Y1 - 2019/3/1

N2 - Background: Before introducing proteins from new or alternative dietary sources into the market, a compressive risk assessment including food allergic sensitization should be carried out in order to ensure their safety. We have recently proposed the adverse outcome pathway (AOP) concept to structure the current mechanistic understanding of the molecular and cellular pathways evidenced to drive IgE-mediated food allergies. This AOP framework offers the biological context to collect and structure existing in vitro methods and to identify missing assays to evaluate sensitizing potential of food proteins. Scope and approach: In this review, we provide a state-of-the-art overview of available in vitro approaches for assessing the sensitizing potential of food proteins, including their strengths and limitations. These approaches are structured by their potential to evaluate the molecular initiating and key events driving food sensitization. Key findings and conclusions: The application of the AOP framework offers the opportunity to anchor existing testing methods to specific building blocks of the AOP for food sensitization. In general, in vitro methods evaluating mechanisms involved in the innate immune response are easier to address than assays addressing the adaptive immune response due to the low precursor frequency of allergen-specific T and B cells. Novel ex vivo culture strategies may have the potential to become useful tools for investigating the sensitizing potential of food proteins. When applied in the context of an integrated testing strategy, the described approaches may reduce, if not replace, current animal testing approaches.

AB - Background: Before introducing proteins from new or alternative dietary sources into the market, a compressive risk assessment including food allergic sensitization should be carried out in order to ensure their safety. We have recently proposed the adverse outcome pathway (AOP) concept to structure the current mechanistic understanding of the molecular and cellular pathways evidenced to drive IgE-mediated food allergies. This AOP framework offers the biological context to collect and structure existing in vitro methods and to identify missing assays to evaluate sensitizing potential of food proteins. Scope and approach: In this review, we provide a state-of-the-art overview of available in vitro approaches for assessing the sensitizing potential of food proteins, including their strengths and limitations. These approaches are structured by their potential to evaluate the molecular initiating and key events driving food sensitization. Key findings and conclusions: The application of the AOP framework offers the opportunity to anchor existing testing methods to specific building blocks of the AOP for food sensitization. In general, in vitro methods evaluating mechanisms involved in the innate immune response are easier to address than assays addressing the adaptive immune response due to the low precursor frequency of allergen-specific T and B cells. Novel ex vivo culture strategies may have the potential to become useful tools for investigating the sensitizing potential of food proteins. When applied in the context of an integrated testing strategy, the described approaches may reduce, if not replace, current animal testing approaches.

KW - Adverse outcome pathway

KW - Dendritic cells

KW - Epithelial cells

KW - IgE-mediated food allergy

KW - In vitro models

KW - T and B cells

U2 - 10.1016/j.tifs.2019.01.014

DO - 10.1016/j.tifs.2019.01.014

M3 - Review article

VL - 85

SP - 307

EP - 319

JO - Trends in Food Science and Technology

JF - Trends in Food Science and Technology

SN - 0924-2244

ER -