Application of an immunosensor for the detection of the beta-lactam antibiotic, cephalexin

P.P. Dillon, S.J. Daly, S.J. Browne, B.M. Manning, E.E.M.G. Loomans, A. van Amerongen, R. O'Kennedy

    Research output: Contribution to journalArticleAcademicpeer-review

    13 Citations (Scopus)

    Abstract

    Public concern surrounding antibiotic contamination in food and food products has made it imperative to develop analytical methods for their detection. Polyclonal antibodies were used in the development of a surface plasmon resonance (SPR)-based inhibition immunoassay for cephalexin. A conjugate consisting of cephalexin-bovine serum albumin (BSA) was immobilized on the dextran gel surface of the sensor chip. Binding/regeneration studies of antibody to immobilized cephalexin were studied and dissociation of the antibody from the immobilized cephalexin was easily achieved with 10 mmol l¿1 NaOH. Forty surface regeneration cycles were carried out and found to be reproducible with only a 7.4% decrease in binding over this number of regenerations. Model inhibition immunoassays for cephalexin were developed in PBS and spiked milk samples with detection ranges of 4.88 to 2,500 ng ml¿1 and 244 to 3,906 pg ml¿1, respectively.
    Original languageEnglish
    Pages (from-to)225-234
    JournalFood and Agricultural Immunology
    Volume15
    Issue number3-4
    DOIs
    Publication statusPublished - 2003

    Fingerprint

    cephalexin
    beta-lactam antibiotics
    Cephalexin
    beta-Lactams
    Anti-Bacterial Agents
    Immobilized Antibodies
    Regeneration
    immunoassays
    Immunoassay
    Immunologic Techniques
    Food Contamination
    surface plasmon resonance
    antibodies
    Surface Plasmon Resonance
    food contamination
    dextran
    bovine serum albumin
    Bovine Serum Albumin
    Dextrans
    polyclonal antibodies

    Keywords

    • surface-plasmon resonance
    • monoclonal-antibody
    • enzyme-immunoassay
    • biosensor assay
    • raw-milk
    • residues
    • serum
    • validation

    Cite this

    Dillon, P. P., Daly, S. J., Browne, S. J., Manning, B. M., Loomans, E. E. M. G., van Amerongen, A., & O'Kennedy, R. (2003). Application of an immunosensor for the detection of the beta-lactam antibiotic, cephalexin. Food and Agricultural Immunology, 15(3-4), 225-234. https://doi.org/10.1080/09540100400003246
    Dillon, P.P. ; Daly, S.J. ; Browne, S.J. ; Manning, B.M. ; Loomans, E.E.M.G. ; van Amerongen, A. ; O'Kennedy, R. / Application of an immunosensor for the detection of the beta-lactam antibiotic, cephalexin. In: Food and Agricultural Immunology. 2003 ; Vol. 15, No. 3-4. pp. 225-234.
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    title = "Application of an immunosensor for the detection of the beta-lactam antibiotic, cephalexin",
    abstract = "Public concern surrounding antibiotic contamination in food and food products has made it imperative to develop analytical methods for their detection. Polyclonal antibodies were used in the development of a surface plasmon resonance (SPR)-based inhibition immunoassay for cephalexin. A conjugate consisting of cephalexin-bovine serum albumin (BSA) was immobilized on the dextran gel surface of the sensor chip. Binding/regeneration studies of antibody to immobilized cephalexin were studied and dissociation of the antibody from the immobilized cephalexin was easily achieved with 10 mmol l¿1 NaOH. Forty surface regeneration cycles were carried out and found to be reproducible with only a 7.4{\%} decrease in binding over this number of regenerations. Model inhibition immunoassays for cephalexin were developed in PBS and spiked milk samples with detection ranges of 4.88 to 2,500 ng ml¿1 and 244 to 3,906 pg ml¿1, respectively.",
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    language = "English",
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    Application of an immunosensor for the detection of the beta-lactam antibiotic, cephalexin. / Dillon, P.P.; Daly, S.J.; Browne, S.J.; Manning, B.M.; Loomans, E.E.M.G.; van Amerongen, A.; O'Kennedy, R.

    In: Food and Agricultural Immunology, Vol. 15, No. 3-4, 2003, p. 225-234.

    Research output: Contribution to journalArticleAcademicpeer-review

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    AU - Daly, S.J.

    AU - Browne, S.J.

    AU - Manning, B.M.

    AU - Loomans, E.E.M.G.

    AU - van Amerongen, A.

    AU - O'Kennedy, R.

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    AB - Public concern surrounding antibiotic contamination in food and food products has made it imperative to develop analytical methods for their detection. Polyclonal antibodies were used in the development of a surface plasmon resonance (SPR)-based inhibition immunoassay for cephalexin. A conjugate consisting of cephalexin-bovine serum albumin (BSA) was immobilized on the dextran gel surface of the sensor chip. Binding/regeneration studies of antibody to immobilized cephalexin were studied and dissociation of the antibody from the immobilized cephalexin was easily achieved with 10 mmol l¿1 NaOH. Forty surface regeneration cycles were carried out and found to be reproducible with only a 7.4% decrease in binding over this number of regenerations. Model inhibition immunoassays for cephalexin were developed in PBS and spiked milk samples with detection ranges of 4.88 to 2,500 ng ml¿1 and 244 to 3,906 pg ml¿1, respectively.

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    KW - biosensor assay

    KW - raw-milk

    KW - residues

    KW - serum

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