Application of a luminescent bacterial biosensor for the detection of tetracyclines in routine analysis of poultry muscle samples

M.G. Pikkemaat, M.L.B.A. Rapallini, M.T. Karp, J.W.A. Elferink

    Research output: Contribution to journalArticleAcademicpeer-review

    19 Citations (Scopus)

    Abstract

    Tetracyclines are extensively used in veterinary medicine. For the detection of tetracycline residues in animal products, a broad array of methods is available. Luminescent bacterial biosensors represent an attractive inexpensive, simple and fast method for screening large numbers of samples. A previously developed cell-biosensor method was subjected to an evaluation study using over 300 routine poultry samples and the results were compared with a microbial inhibition test. The cell-biosensor assay yielded many more suspect samples, 10.2% versus 2% with the inhibition test, which all could be confirmed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Only one sample contained a concentration above the maximum residue limit (MRL) of 100 mu g kg-1, while residue levels in most of the suspect samples were very low (10 mu g kg-1). The method appeared to be specific and robust. Using an experimental set-up comprising the analysis of a series of three sample dilutions allowed an appropriate cut-off for confirmatory analysis, limiting the number of samples and requiring further analysis to a minimum
    Original languageEnglish
    Pages (from-to)1112-1117
    JournalFood Additives & Contaminants. Pt. A, Chemistry, Analysis, Control, Exposure & Risk Assessment
    Volume27
    Issue number8
    DOIs
    Publication statusPublished - 2010

    Keywords

    • screening methods
    • sensor strain
    • antibiotics
    • residues
    • kidney
    • genes
    • assay
    • meat

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