Abstract
Tetracyclines are extensively used in veterinary medicine. For the detection of tetracycline residues in animal products, a broad array of methods is available. Luminescent bacterial biosensors represent an attractive inexpensive, simple and fast method for screening large numbers of samples. A previously developed cell-biosensor method was subjected to an evaluation study using over 300 routine poultry samples and the results were compared with a microbial inhibition test. The cell-biosensor assay yielded many more suspect samples, 10.2% versus 2% with the inhibition test, which all could be confirmed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Only one sample contained a concentration above the maximum residue limit (MRL) of 100 mu g kg-1, while residue levels in most of the suspect samples were very low (10 mu g kg-1). The method appeared to be specific and robust. Using an experimental set-up comprising the analysis of a series of three sample dilutions allowed an appropriate cut-off for confirmatory analysis, limiting the number of samples and requiring further analysis to a minimum
Original language | English |
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Pages (from-to) | 1112-1117 |
Journal | Food Additives & Contaminants. Pt. A, Chemistry, Analysis, Control, Exposure & Risk Assessment |
Volume | 27 |
Issue number | 8 |
DOIs | |
Publication status | Published - 2010 |
Keywords
- screening methods
- sensor strain
- antibiotics
- residues
- kidney
- genes
- assay
- meat