Abstract
The aim of the present study was to demonstrate
the applicability of a yeast androgen and estrogen bioassay
in the detection of steroid esters in hair samples of animals
treated with a hormone ester cocktail. The outcome of the
activity screenings was critically compared with the results
previously obtained with LC-MS/MS analysis. Hair samples
of one pour-on treated animal, 10 ml DMSO containing
25 mg estradiol benzoate (EB), 60 mg testosterone
decanoate (TD) and 60 mg testosterone cypionate (TC),
were selected and analyzed with the androgen and estrogen
yeast bioassay. Results showed that by the introduction of a
hydrolysis step, bioassays can be used to screen for the
presence of hormone esters in hair samples. Based on the
difference in fluorescence responses between the nonhydrolyzed
and the hydrolyzed hair samples, it was
possible to detect the presence of EB up to at least 56 days
after a single pour-on treatment and to detect the presence
of TC and TD up to at least 14 days after the treatment.
Although the LC-MS/MS analysis could detect TC and TD
up to 49 days after treatment, bioassays have the advantage
that they can also detect any (un)known steroid ester.
Keywords Testosterone ester . Estradiol benzoate . Yeast
bioassay . Untargeted analysis . Hair
| Original language | English |
|---|---|
| Pages (from-to) | 1031-1039 |
| Journal | Analytical and Bioanalytical Chemistry |
| Volume | 399 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2011 |
Keywords
- green fluorescent protein
- tandem mass-spectrometry
- estrogenic activity
- bovine hair
- estradiol benzoate
- anabolic-steroids
- calf urine
- validation
- samples
- expression