Apple polyphenol extracts prevent aspirin-induced damage to the rat gastric mucosa

Giuseppe D'Argenio*, Giovanna Mazzone, Concetta Tuccillo, Ilenia Grandone, Antonietta G. Gravina, Giulia Graziani, Vincenzo Fogliano, Marco Romano

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

42 Citations (Scopus)

Abstract

Aspirin causes gastroduodenal ulcers and complications. Food bioactive compounds could exert beneficial effects in the gastrointestinal tract. We evaluated whether apple polyphenol extract (APE) reduced aspirin-induced injury to the rat gastric mucosa. Rats were treated with APE (104 M catechin equivalent) before oral aspirin (200 mg/kg). Cyclo-oxygenase-2 (COX-2), transforming growth factor-α (TGFα) and heparin-binding epidermal-growth-factor-like growth factor (HB-EGF) mRNA and protein expression were assessed by RT-PCR and Western blot analysis, respectively; malondialdehyde (MDA) was determined by HPLC; gastric secretion was evaluated in pylorus-ligated rats. APE decreased acute and chronic aspirin injury both macroscopically and microscopically (approximately 50% decrease in lesion score; P < 0. 05). Aspirin up-regulated mRNA and protein expression of COX-2 and HB-EGF, but not of TGFα APE reduced aspirin-induced mRNA and protein over-expression of COX-2 and HB-EGF; aspirin significantly increased gastric MDA and this effect was counteracted by APE pre-treatment. APE did not significantly affect gastric acid secretion. In conclusion, APE reduces aspirin-induced gastric injury independently of acid inhibition. We speculate that APE might be of therapeutic use in the prophylaxis of aspirin-related gastropathy.

Original languageEnglish
Pages (from-to)1228-1236
Number of pages9
JournalBritish Journal of Nutrition
Volume100
Issue number6
DOIs
Publication statusPublished - 1 Dec 2008
Externally publishedYes

Keywords

  • Apple polyphenols
  • Aspirin
  • Gastric injury

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