Aphid transmission of beet western yellows luteovirus requires the minor capsid read-through protein P74

V. Brault, J.F.J.M. van den Heuvel, M. Verbeek, V. Ziegler-Graff, A. Reutenauer, E. Herrbach, J.C. Garaud, H. Guilley, K. Richards, G. Jonard

    Research output: Contribution to journalArticleAcademicpeer-review

    165 Citations (Scopus)

    Abstract

    Beet western yellows luteovirus is obligately transmitted by the aphid Myzus persicae in a circulative, non-propagative fashion. Virus movement across the epithelial cells of the digestive tube into the hemocoel and from the hemocoel into the accessory salivary glands is believed to occur by receptor-mediated endocytosis and exocytosis. Virions contain two types of protein; the major 22 kDa capsid protein and the minor read-through protein, P74, which is composed of the major capsid protein fused by translational read-through to a long C-terminal extension called the read-through domain. Beet western yellows virus carrying various mutations in the read-through domain was tested for its ability to be transmitted to test plants by aphids fed on agro-infected plants and semi-purified or purified virus preparations. The results establish that the read-through domain carries determinants that are essential for aphid transmission. The findings also reveal that the read-through domain is important for accumulation of the virus in agro-infected plants.
    Original languageEnglish
    Pages (from-to)650-659
    JournalThe EMBO Journal
    Volume14
    Issue number4
    Publication statusPublished - 1995

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