Aphid resistance in florist's chrysanthemum (Chrysanthemum morifolium Ramat.) induced by sea anemone equistatin overexpression

M. Valizadeh, C. Deraison, S.K. Kazemitabar, Y. Rahbé, M.A. Jongsma

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Florist's chrysanthemum (Chrysanthemum morifolium Ramat.) belongs to the Asteraceae family and represents the second most important floricultural crop in the world. Most genotypes are sensitive to aphids and infestations can lower quality and cause transmission of viruses. The protease inhibitor Sea Anemone Equistatin (SAE) carries three domains responsible for the inhibition of both cysteine and aspartic proteases. Artificial diet bioassays showed that SAE is readily toxic when ingested by the pea aphid, Acyrthosiphon pisum, and the cotton aphid, Aphis gossypii. We transformed chrysanthemum genotype 1581 by Agrobacterium tumefaciens-mediated transformation with the SAE gene under the control of the chrysanthemum RbcS promoter to induce aphid resistance. Non-choice leaf disk and whole plant bioassays were carried out to analyze deleterious effects of SAE on population growth and survival of both Myzus persicae and A. gossypii. After 7 days, M. persicae populations on specific transgenic lines were up to 69% smaller relative to control populations in a whole plant bioassay. The mortality of cotton aphids was 11% on control lines and up to 32% on transgenic lines after 5 days. The results show that SAE may be a promising agent for the control of some aphid species in transgenic plants. Key words: Chrysanthemum morifolium, aphid resistance, RbcS promoter, sea anemone equistatin, agrobacterium transformation.
Original languageEnglish
Pages (from-to)6922-6930
JournalAfrican journal of biotechnology
Volume12
Issue number50
DOIs
Publication statusPublished - 2013

Fingerprint

Chrysanthemum
Sea Anemones
Aphids
Chrysanthemum morifolium
Anthozoa
Aphidoidea
Aphis gossypii
Acyrthosiphon pisum
bioassays
Myzus persicae
Biological Assay
promoter regions
genetically modified organisms
floriculture crops
genotype
cysteine proteinases
Genotype
virus transmission
artificial diets
proteinase inhibitors

Cite this

@article{21bfe3aad1454f0bbbf5c9fdb84ae9ef,
title = "Aphid resistance in florist's chrysanthemum (Chrysanthemum morifolium Ramat.) induced by sea anemone equistatin overexpression",
abstract = "Florist's chrysanthemum (Chrysanthemum morifolium Ramat.) belongs to the Asteraceae family and represents the second most important floricultural crop in the world. Most genotypes are sensitive to aphids and infestations can lower quality and cause transmission of viruses. The protease inhibitor Sea Anemone Equistatin (SAE) carries three domains responsible for the inhibition of both cysteine and aspartic proteases. Artificial diet bioassays showed that SAE is readily toxic when ingested by the pea aphid, Acyrthosiphon pisum, and the cotton aphid, Aphis gossypii. We transformed chrysanthemum genotype 1581 by Agrobacterium tumefaciens-mediated transformation with the SAE gene under the control of the chrysanthemum RbcS promoter to induce aphid resistance. Non-choice leaf disk and whole plant bioassays were carried out to analyze deleterious effects of SAE on population growth and survival of both Myzus persicae and A. gossypii. After 7 days, M. persicae populations on specific transgenic lines were up to 69{\%} smaller relative to control populations in a whole plant bioassay. The mortality of cotton aphids was 11{\%} on control lines and up to 32{\%} on transgenic lines after 5 days. The results show that SAE may be a promising agent for the control of some aphid species in transgenic plants. Key words: Chrysanthemum morifolium, aphid resistance, RbcS promoter, sea anemone equistatin, agrobacterium transformation.",
author = "M. Valizadeh and C. Deraison and S.K. Kazemitabar and Y. Rahb{\'e} and M.A. Jongsma",
year = "2013",
doi = "10.5897/AJB2013.12956",
language = "English",
volume = "12",
pages = "6922--6930",
journal = "African journal of biotechnology",
issn = "1684-5315",
publisher = "Academic Journals",
number = "50",

}

Aphid resistance in florist's chrysanthemum (Chrysanthemum morifolium Ramat.) induced by sea anemone equistatin overexpression. / Valizadeh, M.; Deraison, C.; Kazemitabar, S.K.; Rahbé, Y.; Jongsma, M.A.

In: African journal of biotechnology, Vol. 12, No. 50, 2013, p. 6922-6930.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Aphid resistance in florist's chrysanthemum (Chrysanthemum morifolium Ramat.) induced by sea anemone equistatin overexpression

AU - Valizadeh, M.

AU - Deraison, C.

AU - Kazemitabar, S.K.

AU - Rahbé, Y.

AU - Jongsma, M.A.

PY - 2013

Y1 - 2013

N2 - Florist's chrysanthemum (Chrysanthemum morifolium Ramat.) belongs to the Asteraceae family and represents the second most important floricultural crop in the world. Most genotypes are sensitive to aphids and infestations can lower quality and cause transmission of viruses. The protease inhibitor Sea Anemone Equistatin (SAE) carries three domains responsible for the inhibition of both cysteine and aspartic proteases. Artificial diet bioassays showed that SAE is readily toxic when ingested by the pea aphid, Acyrthosiphon pisum, and the cotton aphid, Aphis gossypii. We transformed chrysanthemum genotype 1581 by Agrobacterium tumefaciens-mediated transformation with the SAE gene under the control of the chrysanthemum RbcS promoter to induce aphid resistance. Non-choice leaf disk and whole plant bioassays were carried out to analyze deleterious effects of SAE on population growth and survival of both Myzus persicae and A. gossypii. After 7 days, M. persicae populations on specific transgenic lines were up to 69% smaller relative to control populations in a whole plant bioassay. The mortality of cotton aphids was 11% on control lines and up to 32% on transgenic lines after 5 days. The results show that SAE may be a promising agent for the control of some aphid species in transgenic plants. Key words: Chrysanthemum morifolium, aphid resistance, RbcS promoter, sea anemone equistatin, agrobacterium transformation.

AB - Florist's chrysanthemum (Chrysanthemum morifolium Ramat.) belongs to the Asteraceae family and represents the second most important floricultural crop in the world. Most genotypes are sensitive to aphids and infestations can lower quality and cause transmission of viruses. The protease inhibitor Sea Anemone Equistatin (SAE) carries three domains responsible for the inhibition of both cysteine and aspartic proteases. Artificial diet bioassays showed that SAE is readily toxic when ingested by the pea aphid, Acyrthosiphon pisum, and the cotton aphid, Aphis gossypii. We transformed chrysanthemum genotype 1581 by Agrobacterium tumefaciens-mediated transformation with the SAE gene under the control of the chrysanthemum RbcS promoter to induce aphid resistance. Non-choice leaf disk and whole plant bioassays were carried out to analyze deleterious effects of SAE on population growth and survival of both Myzus persicae and A. gossypii. After 7 days, M. persicae populations on specific transgenic lines were up to 69% smaller relative to control populations in a whole plant bioassay. The mortality of cotton aphids was 11% on control lines and up to 32% on transgenic lines after 5 days. The results show that SAE may be a promising agent for the control of some aphid species in transgenic plants. Key words: Chrysanthemum morifolium, aphid resistance, RbcS promoter, sea anemone equistatin, agrobacterium transformation.

U2 - 10.5897/AJB2013.12956

DO - 10.5897/AJB2013.12956

M3 - Article

VL - 12

SP - 6922

EP - 6930

JO - African journal of biotechnology

JF - African journal of biotechnology

SN - 1684-5315

IS - 50

ER -