Antisense down-regulation of strawberry endo-beta (1,4)-glucanase genes does not prevent fruit softening during ripening

X. Palomer, I. Llop-Tous, M. Vendrell, F.A. Krens, J.G. Schaart, M.J. Boone, H.C.P.M. van der Valk, E.M.J. Salentijn

    Research output: Contribution to journalArticleAcademicpeer-review

    33 Citations (Scopus)

    Abstract

    Strawberry (Fragaria × ananassa Duch.) fruit softening during ripening is associated with the overlapping presence of two divergent endo-ß-(1,4)-glucanases (EC 3.2.1.4; EGases), Cel1 and Cel2. Antisense down-regulation of both genes was performed to assess the precise role of these endo-ß-(1,4)-glucanases on strawberry fruit softening. Constant down-regulation of cel1 expression throughout ripening was obtained, which was accompanied by reduced Cel1 protein accumulation. However, diminution of the Cel1 protein level together with a reduction of the total EGase activity to 40% of the control level did not affect fruit firmness, thus suggesting that Cel1 protein is not the major determinant of fruit softening during ripening. On the other hand, no significant reduction of Cel2 protein accumulation was found in any of the Cel2 transgenic or Cel1/Cel2 double-transgenic lines obtained. The difficulties encountered to yield a strawberry line with significant reduction of cel2 expression suggest that this gene might be playing a pivotal role on fruit development prior to ripening, thus accounting for the lack of Cel2 protein down-regulation observed
    Original languageEnglish
    Pages (from-to)640-646
    JournalPlant Science
    Volume171
    Issue number5
    DOIs
    Publication statusPublished - 2006

    Fingerprint

    Fragaria
    Cellulase
    endo-1,4-beta-glucanase
    strawberries
    Fruit
    ripening
    Down-Regulation
    fruits
    Genes
    Proteins
    genes
    proteins
    genetically modified organisms
    Fragaria ananassa
    fruiting
    firmness
    Gene Expression
    gene expression

    Keywords

    • cell-wall polysaccharides
    • transgenic tomato fruit
    • fragaria x ananassa
    • molecular characterization
    • pectate lyase
    • higher-plants
    • expression
    • endo-1,4-beta-glucanase
    • endo-beta-1,4-glucanase
    • suppression

    Cite this

    Palomer, X. ; Llop-Tous, I. ; Vendrell, M. ; Krens, F.A. ; Schaart, J.G. ; Boone, M.J. ; van der Valk, H.C.P.M. ; Salentijn, E.M.J. / Antisense down-regulation of strawberry endo-beta (1,4)-glucanase genes does not prevent fruit softening during ripening. In: Plant Science. 2006 ; Vol. 171, No. 5. pp. 640-646.
    @article{69bb9f7a24d345a490dcb412c41abce5,
    title = "Antisense down-regulation of strawberry endo-beta (1,4)-glucanase genes does not prevent fruit softening during ripening",
    abstract = "Strawberry (Fragaria × ananassa Duch.) fruit softening during ripening is associated with the overlapping presence of two divergent endo-{\ss}-(1,4)-glucanases (EC 3.2.1.4; EGases), Cel1 and Cel2. Antisense down-regulation of both genes was performed to assess the precise role of these endo-{\ss}-(1,4)-glucanases on strawberry fruit softening. Constant down-regulation of cel1 expression throughout ripening was obtained, which was accompanied by reduced Cel1 protein accumulation. However, diminution of the Cel1 protein level together with a reduction of the total EGase activity to 40{\%} of the control level did not affect fruit firmness, thus suggesting that Cel1 protein is not the major determinant of fruit softening during ripening. On the other hand, no significant reduction of Cel2 protein accumulation was found in any of the Cel2 transgenic or Cel1/Cel2 double-transgenic lines obtained. The difficulties encountered to yield a strawberry line with significant reduction of cel2 expression suggest that this gene might be playing a pivotal role on fruit development prior to ripening, thus accounting for the lack of Cel2 protein down-regulation observed",
    keywords = "cell-wall polysaccharides, transgenic tomato fruit, fragaria x ananassa, molecular characterization, pectate lyase, higher-plants, expression, endo-1,4-beta-glucanase, endo-beta-1,4-glucanase, suppression",
    author = "X. Palomer and I. Llop-Tous and M. Vendrell and F.A. Krens and J.G. Schaart and M.J. Boone and {van der Valk}, H.C.P.M. and E.M.J. Salentijn",
    note = "in press",
    year = "2006",
    doi = "10.1016/j.plantsci.2006.06.019",
    language = "English",
    volume = "171",
    pages = "640--646",
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    Antisense down-regulation of strawberry endo-beta (1,4)-glucanase genes does not prevent fruit softening during ripening. / Palomer, X.; Llop-Tous, I.; Vendrell, M.; Krens, F.A.; Schaart, J.G.; Boone, M.J.; van der Valk, H.C.P.M.; Salentijn, E.M.J.

    In: Plant Science, Vol. 171, No. 5, 2006, p. 640-646.

    Research output: Contribution to journalArticleAcademicpeer-review

    TY - JOUR

    T1 - Antisense down-regulation of strawberry endo-beta (1,4)-glucanase genes does not prevent fruit softening during ripening

    AU - Palomer, X.

    AU - Llop-Tous, I.

    AU - Vendrell, M.

    AU - Krens, F.A.

    AU - Schaart, J.G.

    AU - Boone, M.J.

    AU - van der Valk, H.C.P.M.

    AU - Salentijn, E.M.J.

    N1 - in press

    PY - 2006

    Y1 - 2006

    N2 - Strawberry (Fragaria × ananassa Duch.) fruit softening during ripening is associated with the overlapping presence of two divergent endo-ß-(1,4)-glucanases (EC 3.2.1.4; EGases), Cel1 and Cel2. Antisense down-regulation of both genes was performed to assess the precise role of these endo-ß-(1,4)-glucanases on strawberry fruit softening. Constant down-regulation of cel1 expression throughout ripening was obtained, which was accompanied by reduced Cel1 protein accumulation. However, diminution of the Cel1 protein level together with a reduction of the total EGase activity to 40% of the control level did not affect fruit firmness, thus suggesting that Cel1 protein is not the major determinant of fruit softening during ripening. On the other hand, no significant reduction of Cel2 protein accumulation was found in any of the Cel2 transgenic or Cel1/Cel2 double-transgenic lines obtained. The difficulties encountered to yield a strawberry line with significant reduction of cel2 expression suggest that this gene might be playing a pivotal role on fruit development prior to ripening, thus accounting for the lack of Cel2 protein down-regulation observed

    AB - Strawberry (Fragaria × ananassa Duch.) fruit softening during ripening is associated with the overlapping presence of two divergent endo-ß-(1,4)-glucanases (EC 3.2.1.4; EGases), Cel1 and Cel2. Antisense down-regulation of both genes was performed to assess the precise role of these endo-ß-(1,4)-glucanases on strawberry fruit softening. Constant down-regulation of cel1 expression throughout ripening was obtained, which was accompanied by reduced Cel1 protein accumulation. However, diminution of the Cel1 protein level together with a reduction of the total EGase activity to 40% of the control level did not affect fruit firmness, thus suggesting that Cel1 protein is not the major determinant of fruit softening during ripening. On the other hand, no significant reduction of Cel2 protein accumulation was found in any of the Cel2 transgenic or Cel1/Cel2 double-transgenic lines obtained. The difficulties encountered to yield a strawberry line with significant reduction of cel2 expression suggest that this gene might be playing a pivotal role on fruit development prior to ripening, thus accounting for the lack of Cel2 protein down-regulation observed

    KW - cell-wall polysaccharides

    KW - transgenic tomato fruit

    KW - fragaria x ananassa

    KW - molecular characterization

    KW - pectate lyase

    KW - higher-plants

    KW - expression

    KW - endo-1,4-beta-glucanase

    KW - endo-beta-1,4-glucanase

    KW - suppression

    U2 - 10.1016/j.plantsci.2006.06.019

    DO - 10.1016/j.plantsci.2006.06.019

    M3 - Article

    VL - 171

    SP - 640

    EP - 646

    JO - Plant Science

    JF - Plant Science

    SN - 0168-9452

    IS - 5

    ER -