Antigen-dependent effects of divergent selective breeding based on natural antibodies on specific humoral immune responses in chickens

Research output: Contribution to journalArticleAcademicpeer-review

5 Citations (Scopus)

Abstract

NAb are defined as antigen binding antibodies present without a known previous exposure to this antigen. NAb are suggested to enhance specific antibody (SpAb) responses, but consequences of different NAb levels on immunization are largely unknown. Layer chickens were divergently selected and bred for keyhole limpet hemocyanin (KLH)-binding NAb titers, resulting in a High line and a Low line. In this study, we investigated: (1) the relation of NAb levels with SpAb titers; and (2) the effect of immunization on NAb titers. The 50 highest females of the High line and the 50 lowest females of the Low line of generation 2 were intramuscularly immunized at 33 weeks of age with 1 mL phosphate buffered saline (PBS) containing one of four treatments: (1) negative control (no antigen), (2) 500 μg KLH, (3) 100 μg avian tuberculin purified protein derivative of Mycobacterium avium (PPD), or (4) 250 μg human serum albumin (HuSA). IgM and IgG titers of NAb and SpAb in plasma were determined prior to immunization and weekly for 5 weeks post immunization by indirect ELISA. In addition, antibody affinity was investigated. No differences in SpAb and NAb response against KLH and PPD were observed as a consequence of different NAb titers, but increased and prolonged SpAb and NAb titer responses against HuSA were observed for the High line compared to the Low line. Different natural antibody titers did not impair SpAb dynamics and SpAb affinity. NAb titers were not, or for only short-term, affected by immunization. We show here that NAb may enhance SpAb responses, but that this effect is antigen-dependent. We hypothesize that NAb play a role in general disease resistance through enhancement of the humoral adaptive immune response.
Original languageEnglish
Pages (from-to)1444-1452
JournalVaccine
Volume36
Issue number11
DOIs
Publication statusPublished - 7 Mar 2018

Fingerprint

selection methods
Humoral Immunity
humoral immunity
Chickens
Immunization
chickens
antigens
Antigens
antibodies
Antibodies
Tuberculin
immunization
Antibody Formation
Antibody Affinity
Serum Albumin
Mycobacterium avium
Disease Resistance
Adaptive Immunity
Immunoglobulin M
Selective Breeding

Keywords

  • Breeding
  • Chicken
  • General disease resistance
  • Keyhole limpet hemocyanin (KLH)
  • Natural antibody
  • Specific antibody

Cite this

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title = "Antigen-dependent effects of divergent selective breeding based on natural antibodies on specific humoral immune responses in chickens",
abstract = "NAb are defined as antigen binding antibodies present without a known previous exposure to this antigen. NAb are suggested to enhance specific antibody (SpAb) responses, but consequences of different NAb levels on immunization are largely unknown. Layer chickens were divergently selected and bred for keyhole limpet hemocyanin (KLH)-binding NAb titers, resulting in a High line and a Low line. In this study, we investigated: (1) the relation of NAb levels with SpAb titers; and (2) the effect of immunization on NAb titers. The 50 highest females of the High line and the 50 lowest females of the Low line of generation 2 were intramuscularly immunized at 33 weeks of age with 1 mL phosphate buffered saline (PBS) containing one of four treatments: (1) negative control (no antigen), (2) 500 μg KLH, (3) 100 μg avian tuberculin purified protein derivative of Mycobacterium avium (PPD), or (4) 250 μg human serum albumin (HuSA). IgM and IgG titers of NAb and SpAb in plasma were determined prior to immunization and weekly for 5 weeks post immunization by indirect ELISA. In addition, antibody affinity was investigated. No differences in SpAb and NAb response against KLH and PPD were observed as a consequence of different NAb titers, but increased and prolonged SpAb and NAb titer responses against HuSA were observed for the High line compared to the Low line. Different natural antibody titers did not impair SpAb dynamics and SpAb affinity. NAb titers were not, or for only short-term, affected by immunization. We show here that NAb may enhance SpAb responses, but that this effect is antigen-dependent. We hypothesize that NAb play a role in general disease resistance through enhancement of the humoral adaptive immune response.",
keywords = "Breeding, Chicken, General disease resistance, Keyhole limpet hemocyanin (KLH), Natural antibody, Specific antibody",
author = "T.V.L. Berghof and J.A.J. Arts and H. Bovenhuis and A. Lammers and {van der Poel}, J.J. and H.K. Parmentier",
year = "2018",
month = "3",
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language = "English",
volume = "36",
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Antigen-dependent effects of divergent selective breeding based on natural antibodies on specific humoral immune responses in chickens. / Berghof, T.V.L.; Arts, J.A.J.; Bovenhuis, H.; Lammers, A.; van der Poel, J.J.; Parmentier, H.K.

In: Vaccine, Vol. 36, No. 11, 07.03.2018, p. 1444-1452.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Antigen-dependent effects of divergent selective breeding based on natural antibodies on specific humoral immune responses in chickens

AU - Berghof, T.V.L.

AU - Arts, J.A.J.

AU - Bovenhuis, H.

AU - Lammers, A.

AU - van der Poel, J.J.

AU - Parmentier, H.K.

PY - 2018/3/7

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N2 - NAb are defined as antigen binding antibodies present without a known previous exposure to this antigen. NAb are suggested to enhance specific antibody (SpAb) responses, but consequences of different NAb levels on immunization are largely unknown. Layer chickens were divergently selected and bred for keyhole limpet hemocyanin (KLH)-binding NAb titers, resulting in a High line and a Low line. In this study, we investigated: (1) the relation of NAb levels with SpAb titers; and (2) the effect of immunization on NAb titers. The 50 highest females of the High line and the 50 lowest females of the Low line of generation 2 were intramuscularly immunized at 33 weeks of age with 1 mL phosphate buffered saline (PBS) containing one of four treatments: (1) negative control (no antigen), (2) 500 μg KLH, (3) 100 μg avian tuberculin purified protein derivative of Mycobacterium avium (PPD), or (4) 250 μg human serum albumin (HuSA). IgM and IgG titers of NAb and SpAb in plasma were determined prior to immunization and weekly for 5 weeks post immunization by indirect ELISA. In addition, antibody affinity was investigated. No differences in SpAb and NAb response against KLH and PPD were observed as a consequence of different NAb titers, but increased and prolonged SpAb and NAb titer responses against HuSA were observed for the High line compared to the Low line. Different natural antibody titers did not impair SpAb dynamics and SpAb affinity. NAb titers were not, or for only short-term, affected by immunization. We show here that NAb may enhance SpAb responses, but that this effect is antigen-dependent. We hypothesize that NAb play a role in general disease resistance through enhancement of the humoral adaptive immune response.

AB - NAb are defined as antigen binding antibodies present without a known previous exposure to this antigen. NAb are suggested to enhance specific antibody (SpAb) responses, but consequences of different NAb levels on immunization are largely unknown. Layer chickens were divergently selected and bred for keyhole limpet hemocyanin (KLH)-binding NAb titers, resulting in a High line and a Low line. In this study, we investigated: (1) the relation of NAb levels with SpAb titers; and (2) the effect of immunization on NAb titers. The 50 highest females of the High line and the 50 lowest females of the Low line of generation 2 were intramuscularly immunized at 33 weeks of age with 1 mL phosphate buffered saline (PBS) containing one of four treatments: (1) negative control (no antigen), (2) 500 μg KLH, (3) 100 μg avian tuberculin purified protein derivative of Mycobacterium avium (PPD), or (4) 250 μg human serum albumin (HuSA). IgM and IgG titers of NAb and SpAb in plasma were determined prior to immunization and weekly for 5 weeks post immunization by indirect ELISA. In addition, antibody affinity was investigated. No differences in SpAb and NAb response against KLH and PPD were observed as a consequence of different NAb titers, but increased and prolonged SpAb and NAb titer responses against HuSA were observed for the High line compared to the Low line. Different natural antibody titers did not impair SpAb dynamics and SpAb affinity. NAb titers were not, or for only short-term, affected by immunization. We show here that NAb may enhance SpAb responses, but that this effect is antigen-dependent. We hypothesize that NAb play a role in general disease resistance through enhancement of the humoral adaptive immune response.

KW - Breeding

KW - Chicken

KW - General disease resistance

KW - Keyhole limpet hemocyanin (KLH)

KW - Natural antibody

KW - Specific antibody

U2 - 10.1016/j.vaccine.2018.01.063

DO - 10.1016/j.vaccine.2018.01.063

M3 - Article

VL - 36

SP - 1444

EP - 1452

JO - Vaccine

JF - Vaccine

SN - 0264-410X

IS - 11

ER -