Antifungal activity of synthetic peptides derived from Impatiens balsamina antimicrobial peptides Ib-AMP1 and Ib-AMP4

K. Thevissen; E.J.A. Francois; L. Sijtsma; A. van Amerongen; W.M.M. Schaaper; R. Meloen; G.A. Posthuma-Trumpie; W.F. Broekaert; B.P.A. Cammue

doi:10.1016/j.peptides.2005.01.008

Antifungal activity of synthetic peptides derived from Impatiens balsamina antimicrobial peptides Ib-AMP1 and Ib-AMP4

K. Thevissen, E.J.A. Francois, L. Sijtsma, A. van Amerongen, W.M.M. Schaaper, R. Meloen, G.A. Posthuma-Trumpie, W.F. Broekaert, B.P.A. Cammue

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55 Citations (Scopus)

Abstract

Seeds of Impatiens balsamina contain a set of related antimicrobial peptides (Ib-AMPs). We have produced a synthetic variant of Ib-AMP1, oxidized to the bicyclic native conformation, which was fully active on yeast and fungal strains; and four linear 20-mer Ib-AMP variants, including two all-d forms. We show that the all-d variants are as active on yeast and fungal strains as native peptides. In addition, fungal growth inhibition nor salt-dependency of Ib-AMP4 could be improved by more than two-fold via replacement of amino acid residues by arginine or tryptophan. Native Ib-AMPs showed no hemolytic nor toxic activity up to a concentration of 100 µM. All these data demonstrate the potential of the native Ib-AMPs to combat fungal infections.

Original language	English
Pages (from-to)	1113-1119
Journal	Peptides
Volume	26
Issue number	7
DOIs	https://doi.org/10.1016/j.peptides.2005.01.008
Publication status	Published - 2005

Keywords

plant defensins
fungal growth
mechanism
proteins

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10.1016/j.peptides.2005.01.008

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title = "Antifungal activity of synthetic peptides derived from Impatiens balsamina antimicrobial peptides Ib-AMP1 and Ib-AMP4",

abstract = "Seeds of Impatiens balsamina contain a set of related antimicrobial peptides (Ib-AMPs). We have produced a synthetic variant of Ib-AMP1, oxidized to the bicyclic native conformation, which was fully active on yeast and fungal strains; and four linear 20-mer Ib-AMP variants, including two all-d forms. We show that the all-d variants are as active on yeast and fungal strains as native peptides. In addition, fungal growth inhibition nor salt-dependency of Ib-AMP4 could be improved by more than two-fold via replacement of amino acid residues by arginine or tryptophan. Native Ib-AMPs showed no hemolytic nor toxic activity up to a concentration of 100 µM. All these data demonstrate the potential of the native Ib-AMPs to combat fungal infections.",

keywords = "plant defensins, fungal growth, mechanism, proteins",

author = "K. Thevissen and E.J.A. Francois and L. Sijtsma and {van Amerongen}, A. and W.M.M. Schaaper and R. Meloen and G.A. Posthuma-Trumpie and W.F. Broekaert and B.P.A. Cammue",

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doi = "10.1016/j.peptides.2005.01.008",

language = "English",

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pages = "1113--1119",

journal = "Peptides",

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TY - JOUR

T1 - Antifungal activity of synthetic peptides derived from Impatiens balsamina antimicrobial peptides Ib-AMP1 and Ib-AMP4

AU - Thevissen, K.

AU - Francois, E.J.A.

AU - Sijtsma, L.

AU - van Amerongen, A.

AU - Schaaper, W.M.M.

AU - Meloen, R.

AU - Posthuma-Trumpie, G.A.

AU - Broekaert, W.F.

AU - Cammue, B.P.A.

PY - 2005

Y1 - 2005

N2 - Seeds of Impatiens balsamina contain a set of related antimicrobial peptides (Ib-AMPs). We have produced a synthetic variant of Ib-AMP1, oxidized to the bicyclic native conformation, which was fully active on yeast and fungal strains; and four linear 20-mer Ib-AMP variants, including two all-d forms. We show that the all-d variants are as active on yeast and fungal strains as native peptides. In addition, fungal growth inhibition nor salt-dependency of Ib-AMP4 could be improved by more than two-fold via replacement of amino acid residues by arginine or tryptophan. Native Ib-AMPs showed no hemolytic nor toxic activity up to a concentration of 100 µM. All these data demonstrate the potential of the native Ib-AMPs to combat fungal infections.

AB - Seeds of Impatiens balsamina contain a set of related antimicrobial peptides (Ib-AMPs). We have produced a synthetic variant of Ib-AMP1, oxidized to the bicyclic native conformation, which was fully active on yeast and fungal strains; and four linear 20-mer Ib-AMP variants, including two all-d forms. We show that the all-d variants are as active on yeast and fungal strains as native peptides. In addition, fungal growth inhibition nor salt-dependency of Ib-AMP4 could be improved by more than two-fold via replacement of amino acid residues by arginine or tryptophan. Native Ib-AMPs showed no hemolytic nor toxic activity up to a concentration of 100 µM. All these data demonstrate the potential of the native Ib-AMPs to combat fungal infections.

KW - plant defensins

KW - fungal growth

KW - mechanism

KW - proteins

U2 - 10.1016/j.peptides.2005.01.008

DO - 10.1016/j.peptides.2005.01.008

M3 - Article

SN - 0196-9781

VL - 26

SP - 1113

EP - 1119

JO - Peptides

JF - Peptides

IS - 7

ER -

Antifungal activity of synthetic peptides derived from Impatiens balsamina antimicrobial peptides Ib-AMP1 and Ib-AMP4

Abstract

Keywords

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