Antifungal activity of synthetic peptides derived from Impatiens balsamina antimicrobial peptides Ib-AMP1 and Ib-AMP4

K. Thevissen, E.J.A. Francois, L. Sijtsma, A. van Amerongen, W.M.M. Schaaper, R. Meloen, G.A. Posthuma-Trumpie, W.F. Broekaert, B.P.A. Cammue

    Research output: Contribution to journalArticleAcademicpeer-review

    42 Citations (Scopus)

    Abstract

    Seeds of Impatiens balsamina contain a set of related antimicrobial peptides (Ib-AMPs). We have produced a synthetic variant of Ib-AMP1, oxidized to the bicyclic native conformation, which was fully active on yeast and fungal strains; and four linear 20-mer Ib-AMP variants, including two all-d forms. We show that the all-d variants are as active on yeast and fungal strains as native peptides. In addition, fungal growth inhibition nor salt-dependency of Ib-AMP4 could be improved by more than two-fold via replacement of amino acid residues by arginine or tryptophan. Native Ib-AMPs showed no hemolytic nor toxic activity up to a concentration of 100 µM. All these data demonstrate the potential of the native Ib-AMPs to combat fungal infections.
    Original languageEnglish
    Pages (from-to)1113-1119
    JournalPeptides
    Volume26
    Issue number7
    DOIs
    Publication statusPublished - 2005

    Keywords

    • plant defensins
    • fungal growth
    • mechanism
    • proteins

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