Antibody orientation on biosensor surfaces: a minireview

A.K. Trilling, M.J. Beekwilder, H. Zuilhof

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197 Citations (Scopus)

Abstract

Detection elements play a key role in analyte recognition in biosensors. Therefore, detection elements with high analyte specificity and binding strength are required. While antibodies (Abs) have been increasingly used as detection elements in biosensors, a key challenge remains – the immobilization on the biosensor surface. This minireview highlights recent approaches to immobilize and study Abs on surfaces. We first introduce Ab species used as detection elements, and discuss techniques recently used to elucidate Ab orientation by determination of layer thickness or surface topology. Then, several immobilization methods will be presented: non-covalent and covalent surface attachment, yielding oriented or random coupled Abs. Finally, protein modification methods applicable for oriented Ab immobilization are reviewed with an eye to future application.
Original languageEnglish
Pages (from-to)1619-1627
JournalThe Analyst
Volume138
Issue number6
DOIs
Publication statusPublished - 2013

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Keywords

  • prostate-specific antigen
  • autodisplayed z-domains
  • protein-g
  • immobilization techniques
  • oriented immobilization
  • recombinant antibodies
  • staudinger ligation
  • monoclonal-antibody
  • escherichia-coli
  • immunoglobulin-g

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