Antibody orientation on biosensor surfaces: a minireview

A.K. Trilling, M.J. Beekwilder, H. Zuilhof

Research output: Contribution to journalReview articleAcademicpeer-review

175 Citations (Scopus)

Abstract

Detection elements play a key role in analyte recognition in biosensors. Therefore, detection elements with high analyte specificity and binding strength are required. While antibodies (Abs) have been increasingly used as detection elements in biosensors, a key challenge remains – the immobilization on the biosensor surface. This minireview highlights recent approaches to immobilize and study Abs on surfaces. We first introduce Ab species used as detection elements, and discuss techniques recently used to elucidate Ab orientation by determination of layer thickness or surface topology. Then, several immobilization methods will be presented: non-covalent and covalent surface attachment, yielding oriented or random coupled Abs. Finally, protein modification methods applicable for oriented Ab immobilization are reviewed with an eye to future application.
LanguageEnglish
Pages1619-1627
JournalThe Analyst
Volume138
Issue number6
DOIs
Publication statusPublished - 2013

Fingerprint

Biosensing Techniques
Biosensors
Antibodies
Immobilization
antibody
immobilization
Introduced Species
topology
Topology
Proteins
protein
detection
method

Keywords

  • prostate-specific antigen
  • autodisplayed z-domains
  • protein-g
  • immobilization techniques
  • oriented immobilization
  • recombinant antibodies
  • staudinger ligation
  • monoclonal-antibody
  • escherichia-coli
  • immunoglobulin-g

Cite this

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abstract = "Detection elements play a key role in analyte recognition in biosensors. Therefore, detection elements with high analyte specificity and binding strength are required. While antibodies (Abs) have been increasingly used as detection elements in biosensors, a key challenge remains – the immobilization on the biosensor surface. This minireview highlights recent approaches to immobilize and study Abs on surfaces. We first introduce Ab species used as detection elements, and discuss techniques recently used to elucidate Ab orientation by determination of layer thickness or surface topology. Then, several immobilization methods will be presented: non-covalent and covalent surface attachment, yielding oriented or random coupled Abs. Finally, protein modification methods applicable for oriented Ab immobilization are reviewed with an eye to future application.",
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Antibody orientation on biosensor surfaces: a minireview. / Trilling, A.K.; Beekwilder, M.J.; Zuilhof, H.

In: The Analyst, Vol. 138, No. 6, 2013, p. 1619-1627.

Research output: Contribution to journalReview articleAcademicpeer-review

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AU - Trilling, A.K.

AU - Beekwilder, M.J.

AU - Zuilhof, H.

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N2 - Detection elements play a key role in analyte recognition in biosensors. Therefore, detection elements with high analyte specificity and binding strength are required. While antibodies (Abs) have been increasingly used as detection elements in biosensors, a key challenge remains – the immobilization on the biosensor surface. This minireview highlights recent approaches to immobilize and study Abs on surfaces. We first introduce Ab species used as detection elements, and discuss techniques recently used to elucidate Ab orientation by determination of layer thickness or surface topology. Then, several immobilization methods will be presented: non-covalent and covalent surface attachment, yielding oriented or random coupled Abs. Finally, protein modification methods applicable for oriented Ab immobilization are reviewed with an eye to future application.

AB - Detection elements play a key role in analyte recognition in biosensors. Therefore, detection elements with high analyte specificity and binding strength are required. While antibodies (Abs) have been increasingly used as detection elements in biosensors, a key challenge remains – the immobilization on the biosensor surface. This minireview highlights recent approaches to immobilize and study Abs on surfaces. We first introduce Ab species used as detection elements, and discuss techniques recently used to elucidate Ab orientation by determination of layer thickness or surface topology. Then, several immobilization methods will be presented: non-covalent and covalent surface attachment, yielding oriented or random coupled Abs. Finally, protein modification methods applicable for oriented Ab immobilization are reviewed with an eye to future application.

KW - prostate-specific antigen

KW - autodisplayed z-domains

KW - protein-g

KW - immobilization techniques

KW - oriented immobilization

KW - recombinant antibodies

KW - staudinger ligation

KW - monoclonal-antibody

KW - escherichia-coli

KW - immunoglobulin-g

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DO - 10.1039/c2an36787d

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