The anti-inflammatory activity of the basolateral fraction of Caco-2 cells exposed to a rosemary supercritical extract was examined. Uptake of rosemary extract fractions was tested on Caco-2 cell monolayers (2–12 h incubation times) and the quantification of carnosic acid and carnosol was performed by UPLC-MS/MS. Human macrophages were treated with the basolateral fractions and secretion of TNF-α, IL-1β, IL-6 and IL-10 was measured by ELISA. The fractions obtained after 8 and 12 h in absorption experiments caused a significant reduction in excretion of the pro-inflammatory cytokines. This reduction in cytokine secretion levels corresponded to the amounts of carnosic acid and carnosol in the basolateral fractions. Thus, the basolateral fraction of a rosemary supercritical extract showed an important anti-inflammatory activity, providing the basis for increasing the use of supercritical rosemary extracts for the prevention of inflammatory diseases.
Arranz, E., Mes, J. J., Wichers, H. J., Jaime, L., Reglero, G., & Santoyo, S. (2015). Anti-inflammatory activity of the basolateral fraction of Caco-2 cells exposed to a rosemary supercritical extract. Journal of Functional Foods, 13(1), 384-390. https://doi.org/10.1016/j.jff.2015.01.015