Anti-aphrodisiac compounds of male butterflies increase the risk of egg parasitoid attack by inducing plant synomone production

N.E. Fatouros, F.G. Pashalidou, W.V. Aponte Cordero, J.J.A. van Loon, R. Mumm, M. Dicke, M. Hilker, M.E. Huigens

Research output: Contribution to journalArticleAcademicpeer-review

30 Citations (Scopus)

Abstract

During mating in many butterfly species, males transfer spermatophores that contain anti-aphrodisiacs to females that repel conspecific males. For example, males of the large cabbage white, Pieris brassicae (Lepidoptera: Pieridae), transfer the anti-aphrodisiac, benzyl cyanide (BC) to females. Accessory reproductive gland (ARG) secretion of a mated female P. brassicae that is deposited with an egg clutch contains traces of BC, inducing Brussels sprouts plants (Brassica oleracea var. gemmifera) to arrest certain Trichogramma egg parasitoids. Here, we assessed whether deposition of one egg at a time by the closely related small cabbage white, Pieris rapae, induced B. oleracea var. gemmifera to arrest Trichogramma wasps, and whether this plant synomone is triggered by substances originating from male P. rapae seminal fluid. We showed that plants induced by singly laid eggs of P. rapae arrest T. brassicae wasps three days after butterfly egg deposition. Elicitor activity was present in ARG secretion of mated female butterflies, whereas the secretion of virgin females was inactive. Pieris rapae used a mixture of methyl salicylate (MeSA) and indole as an anti-aphrodisiac. We detected traces of both anti-aphrodisiacal compounds in the ARG secretion of mated female P. rapae, whereas indole was lacking in the secretion of virgin female P. rapae. When applied onto the leaf, indole induced changes in the foliar chemistry that arrested T. brassicae wasps. This study shows that compounds of male seminal fluid incur possible fitness costs for Pieris butterflies by indirectly promoting egg parasitoid attack
Original languageEnglish
Pages (from-to)1373-1381
JournalJournal of Chemical Ecology
Volume35
Issue number11
DOIs
Publication statusPublished - 2009

Fingerprint

Aphrodisiacs
egg parasitoid
Pieris rapae
Butterflies
Pheromones
Accessories
butterfly
butterflies
Ovum
secretion
Wasps
egg
Brassica
Brassica oleracea var. gemmifera
indoles
wasp
Fluids
Clutches
Pieris brassicae
Trichogramma

Keywords

  • pieris-rapae
  • methyl salicylate
  • predatory mite
  • defense responses
  • pinus-sylvestris
  • cabbage white
  • host location
  • insect
  • volatiles
  • oviposition

Cite this

@article{8cc894152ae64f9084c09506da419a88,
title = "Anti-aphrodisiac compounds of male butterflies increase the risk of egg parasitoid attack by inducing plant synomone production",
abstract = "During mating in many butterfly species, males transfer spermatophores that contain anti-aphrodisiacs to females that repel conspecific males. For example, males of the large cabbage white, Pieris brassicae (Lepidoptera: Pieridae), transfer the anti-aphrodisiac, benzyl cyanide (BC) to females. Accessory reproductive gland (ARG) secretion of a mated female P. brassicae that is deposited with an egg clutch contains traces of BC, inducing Brussels sprouts plants (Brassica oleracea var. gemmifera) to arrest certain Trichogramma egg parasitoids. Here, we assessed whether deposition of one egg at a time by the closely related small cabbage white, Pieris rapae, induced B. oleracea var. gemmifera to arrest Trichogramma wasps, and whether this plant synomone is triggered by substances originating from male P. rapae seminal fluid. We showed that plants induced by singly laid eggs of P. rapae arrest T. brassicae wasps three days after butterfly egg deposition. Elicitor activity was present in ARG secretion of mated female butterflies, whereas the secretion of virgin females was inactive. Pieris rapae used a mixture of methyl salicylate (MeSA) and indole as an anti-aphrodisiac. We detected traces of both anti-aphrodisiacal compounds in the ARG secretion of mated female P. rapae, whereas indole was lacking in the secretion of virgin female P. rapae. When applied onto the leaf, indole induced changes in the foliar chemistry that arrested T. brassicae wasps. This study shows that compounds of male seminal fluid incur possible fitness costs for Pieris butterflies by indirectly promoting egg parasitoid attack",
keywords = "pieris-rapae, methyl salicylate, predatory mite, defense responses, pinus-sylvestris, cabbage white, host location, insect, volatiles, oviposition",
author = "N.E. Fatouros and F.G. Pashalidou and {Aponte Cordero}, W.V. and {van Loon}, J.J.A. and R. Mumm and M. Dicke and M. Hilker and M.E. Huigens",
note = "09-3268",
year = "2009",
doi = "10.1007/s10886-009-9714-5",
language = "English",
volume = "35",
pages = "1373--1381",
journal = "Journal of Chemical Ecology",
issn = "0098-0331",
publisher = "Springer Verlag",
number = "11",

}

Anti-aphrodisiac compounds of male butterflies increase the risk of egg parasitoid attack by inducing plant synomone production. / Fatouros, N.E.; Pashalidou, F.G.; Aponte Cordero, W.V.; van Loon, J.J.A.; Mumm, R.; Dicke, M.; Hilker, M.; Huigens, M.E.

In: Journal of Chemical Ecology, Vol. 35, No. 11, 2009, p. 1373-1381.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Anti-aphrodisiac compounds of male butterflies increase the risk of egg parasitoid attack by inducing plant synomone production

AU - Fatouros, N.E.

AU - Pashalidou, F.G.

AU - Aponte Cordero, W.V.

AU - van Loon, J.J.A.

AU - Mumm, R.

AU - Dicke, M.

AU - Hilker, M.

AU - Huigens, M.E.

N1 - 09-3268

PY - 2009

Y1 - 2009

N2 - During mating in many butterfly species, males transfer spermatophores that contain anti-aphrodisiacs to females that repel conspecific males. For example, males of the large cabbage white, Pieris brassicae (Lepidoptera: Pieridae), transfer the anti-aphrodisiac, benzyl cyanide (BC) to females. Accessory reproductive gland (ARG) secretion of a mated female P. brassicae that is deposited with an egg clutch contains traces of BC, inducing Brussels sprouts plants (Brassica oleracea var. gemmifera) to arrest certain Trichogramma egg parasitoids. Here, we assessed whether deposition of one egg at a time by the closely related small cabbage white, Pieris rapae, induced B. oleracea var. gemmifera to arrest Trichogramma wasps, and whether this plant synomone is triggered by substances originating from male P. rapae seminal fluid. We showed that plants induced by singly laid eggs of P. rapae arrest T. brassicae wasps three days after butterfly egg deposition. Elicitor activity was present in ARG secretion of mated female butterflies, whereas the secretion of virgin females was inactive. Pieris rapae used a mixture of methyl salicylate (MeSA) and indole as an anti-aphrodisiac. We detected traces of both anti-aphrodisiacal compounds in the ARG secretion of mated female P. rapae, whereas indole was lacking in the secretion of virgin female P. rapae. When applied onto the leaf, indole induced changes in the foliar chemistry that arrested T. brassicae wasps. This study shows that compounds of male seminal fluid incur possible fitness costs for Pieris butterflies by indirectly promoting egg parasitoid attack

AB - During mating in many butterfly species, males transfer spermatophores that contain anti-aphrodisiacs to females that repel conspecific males. For example, males of the large cabbage white, Pieris brassicae (Lepidoptera: Pieridae), transfer the anti-aphrodisiac, benzyl cyanide (BC) to females. Accessory reproductive gland (ARG) secretion of a mated female P. brassicae that is deposited with an egg clutch contains traces of BC, inducing Brussels sprouts plants (Brassica oleracea var. gemmifera) to arrest certain Trichogramma egg parasitoids. Here, we assessed whether deposition of one egg at a time by the closely related small cabbage white, Pieris rapae, induced B. oleracea var. gemmifera to arrest Trichogramma wasps, and whether this plant synomone is triggered by substances originating from male P. rapae seminal fluid. We showed that plants induced by singly laid eggs of P. rapae arrest T. brassicae wasps three days after butterfly egg deposition. Elicitor activity was present in ARG secretion of mated female butterflies, whereas the secretion of virgin females was inactive. Pieris rapae used a mixture of methyl salicylate (MeSA) and indole as an anti-aphrodisiac. We detected traces of both anti-aphrodisiacal compounds in the ARG secretion of mated female P. rapae, whereas indole was lacking in the secretion of virgin female P. rapae. When applied onto the leaf, indole induced changes in the foliar chemistry that arrested T. brassicae wasps. This study shows that compounds of male seminal fluid incur possible fitness costs for Pieris butterflies by indirectly promoting egg parasitoid attack

KW - pieris-rapae

KW - methyl salicylate

KW - predatory mite

KW - defense responses

KW - pinus-sylvestris

KW - cabbage white

KW - host location

KW - insect

KW - volatiles

KW - oviposition

U2 - 10.1007/s10886-009-9714-5

DO - 10.1007/s10886-009-9714-5

M3 - Article

VL - 35

SP - 1373

EP - 1381

JO - Journal of Chemical Ecology

JF - Journal of Chemical Ecology

SN - 0098-0331

IS - 11

ER -