Anoxic biological production of fuels and of bulk chemicals from second generation feedstocks

J.A.M. de Bont (Inventor), A. Raab (Inventor), M. Schilling (Inventor), M.M. Tamame González (Inventor), M. De los Ángeles Santos García (Inventor), V.A.P. Martins dos Santos (Inventor), R. Arjona Antolín (Inventor), P. Gutiérrez Gómez (Inventor)

Research output: PatentOther research output

Abstract

The present invention relates to modified eukaryotic microbial cells that have been engineered for producing fermentation products such as fatty acids, 1-alcohols alkenes, alkanes and derivatives thereof under anoxic conditions from second generation feedstocks, including at least pentoses. To this end the eukaryotic microbial cells have been modified to express enzymes of the fatty acid β-oxidation cycle in the cytosol of the cell,under anoxic conditions,in the absence of fatty acids and in the presence of a carbon source containing pentoses. The cells are further modified to express enzymes for metabolizing pentose like xylose and arabinose, as well as to express a metabolic route for producing under anoxic conditions, acetyl-CoA from the non-fatty acid carbon source to feed into and drive the β-oxidation cycle in the biosynthetic direction and,to express termination enzymes for conversion of reaction β-oxidation cycle intermediates into the desired fermentation product. The invention further relates to anoxic processes wherein the cells are used to produce fermentation products such as fatty acids, 1-alcohols,alkenes, alkanes or derivatives thereof.
Original languageEnglish
Patent numberWO2014207099
Priority date26/06/13
Publication statusPublished - 31 Dec 2014

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Pentoses
Feedstocks
Fatty Acids
Fermentation
Alkanes
Alkenes
Patents and inventions
Oxidation
Enzymes
Carbon
Alcohols
Derivatives
Acetyl Coenzyme A
Arabinose
Xylose
Acids

Cite this

de Bont, J. A. M., Raab, A., Schilling, M., Tamame González, M. M., De los Ángeles Santos García, M., Martins dos Santos, V. A. P., ... Gutiérrez Gómez, P. (2014). Anoxic biological production of fuels and of bulk chemicals from second generation feedstocks. (Patent No. WO2014207099).
de Bont, J.A.M. (Inventor) ; Raab, A. (Inventor) ; Schilling, M. (Inventor) ; Tamame González, M.M. (Inventor) ; De los Ángeles Santos García, M. (Inventor) ; Martins dos Santos, V.A.P. (Inventor) ; Arjona Antolín, R. (Inventor) ; Gutiérrez Gómez, P. (Inventor). / Anoxic biological production of fuels and of bulk chemicals from second generation feedstocks. Patent No.: WO2014207099.
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abstract = "The present invention relates to modified eukaryotic microbial cells that have been engineered for producing fermentation products such as fatty acids, 1-alcohols alkenes, alkanes and derivatives thereof under anoxic conditions from second generation feedstocks, including at least pentoses. To this end the eukaryotic microbial cells have been modified to express enzymes of the fatty acid β-oxidation cycle in the cytosol of the cell,under anoxic conditions,in the absence of fatty acids and in the presence of a carbon source containing pentoses. The cells are further modified to express enzymes for metabolizing pentose like xylose and arabinose, as well as to express a metabolic route for producing under anoxic conditions, acetyl-CoA from the non-fatty acid carbon source to feed into and drive the β-oxidation cycle in the biosynthetic direction and,to express termination enzymes for conversion of reaction β-oxidation cycle intermediates into the desired fermentation product. The invention further relates to anoxic processes wherein the cells are used to produce fermentation products such as fatty acids, 1-alcohols,alkenes, alkanes or derivatives thereof.",
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de Bont, JAM, Raab, A, Schilling, M, Tamame González, MM, De los Ángeles Santos García, M, Martins dos Santos, VAP, Arjona Antolín, R & Gutiérrez Gómez, P 2014, Anoxic biological production of fuels and of bulk chemicals from second generation feedstocks, Patent No. WO2014207099.

Anoxic biological production of fuels and of bulk chemicals from second generation feedstocks. / de Bont, J.A.M. (Inventor); Raab, A. (Inventor); Schilling, M. (Inventor); Tamame González, M.M. (Inventor); De los Ángeles Santos García, M. (Inventor); Martins dos Santos, V.A.P. (Inventor); Arjona Antolín, R. (Inventor); Gutiérrez Gómez, P. (Inventor).

Patent No.: WO2014207099.

Research output: PatentOther research output

TY - PAT

T1 - Anoxic biological production of fuels and of bulk chemicals from second generation feedstocks

AU - de Bont, J.A.M.

AU - Raab, A.

AU - Schilling, M.

AU - Tamame González, M.M.

AU - De los Ángeles Santos García, M.

AU - Martins dos Santos, V.A.P.

AU - Arjona Antolín, R.

AU - Gutiérrez Gómez, P.

PY - 2014/12/31

Y1 - 2014/12/31

N2 - The present invention relates to modified eukaryotic microbial cells that have been engineered for producing fermentation products such as fatty acids, 1-alcohols alkenes, alkanes and derivatives thereof under anoxic conditions from second generation feedstocks, including at least pentoses. To this end the eukaryotic microbial cells have been modified to express enzymes of the fatty acid β-oxidation cycle in the cytosol of the cell,under anoxic conditions,in the absence of fatty acids and in the presence of a carbon source containing pentoses. The cells are further modified to express enzymes for metabolizing pentose like xylose and arabinose, as well as to express a metabolic route for producing under anoxic conditions, acetyl-CoA from the non-fatty acid carbon source to feed into and drive the β-oxidation cycle in the biosynthetic direction and,to express termination enzymes for conversion of reaction β-oxidation cycle intermediates into the desired fermentation product. The invention further relates to anoxic processes wherein the cells are used to produce fermentation products such as fatty acids, 1-alcohols,alkenes, alkanes or derivatives thereof.

AB - The present invention relates to modified eukaryotic microbial cells that have been engineered for producing fermentation products such as fatty acids, 1-alcohols alkenes, alkanes and derivatives thereof under anoxic conditions from second generation feedstocks, including at least pentoses. To this end the eukaryotic microbial cells have been modified to express enzymes of the fatty acid β-oxidation cycle in the cytosol of the cell,under anoxic conditions,in the absence of fatty acids and in the presence of a carbon source containing pentoses. The cells are further modified to express enzymes for metabolizing pentose like xylose and arabinose, as well as to express a metabolic route for producing under anoxic conditions, acetyl-CoA from the non-fatty acid carbon source to feed into and drive the β-oxidation cycle in the biosynthetic direction and,to express termination enzymes for conversion of reaction β-oxidation cycle intermediates into the desired fermentation product. The invention further relates to anoxic processes wherein the cells are used to produce fermentation products such as fatty acids, 1-alcohols,alkenes, alkanes or derivatives thereof.

M3 - Patent

M1 - WO2014207099

ER -

de Bont JAM, Raab A, Schilling M, Tamame González MM, De los Ángeles Santos García M, Martins dos Santos VAP et al, inventors. Anoxic biological production of fuels and of bulk chemicals from second generation feedstocks. WO2014207099. 2014 Dec 31.