Analysis of the Tomato spotted wilt virus ambisense S RNA-encoded hairpin structure in translation

C. Geerts-Dimitriadou; Y.Y. Lu; C. Geertsema; R.W. Goldbach; R.J.M. Kormelink

doi:10.1371/journal.pone.0031013

Analysis of the Tomato spotted wilt virus ambisense S RNA-encoded hairpin structure in translation

C. Geerts-Dimitriadou, Y.Y. Lu, C. Geertsema, R.W. Goldbach, R.J.M. Kormelink

Laboratory of Virology

Research output: Contribution to journal › Article › Academic › peer-review

28 Citations (Scopus)

Abstract

The intergenic region (IR) of ambisense RNA segments from animal- and plant-infecting (-)RNA viruses functions as a bidirectional transcription terminator. The IR sequence of the Tomato spotted wilt virus (TSWV) ambisense S RNA contains stretches that are highly rich in A-residues and U-residues and is predicted to fold into a stable hairpin structure. The presence of this hairpin structure sequence in the 3' untranslated region (UTR) of TSWV mRNAs implies a possible role in translation.

Original language	English
Article number	e31013
Journal	PLoS ONE
Volume	7
Issue number	2
DOIs	https://doi.org/10.1371/journal.pone.0031013
Publication status	Published - 2012

Keywords

nonstructural protein nss
affecting transcription termination
tail-independent translation
cap-snatching mechanism
escherichia-coli rna
stem-loop structure
cdna-based rescue
messenger-rna
influenza-virus
intergenic region

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title = "Analysis of the Tomato spotted wilt virus ambisense S RNA-encoded hairpin structure in translation",

abstract = "The intergenic region (IR) of ambisense RNA segments from animal- and plant-infecting (-)RNA viruses functions as a bidirectional transcription terminator. The IR sequence of the Tomato spotted wilt virus (TSWV) ambisense S RNA contains stretches that are highly rich in A-residues and U-residues and is predicted to fold into a stable hairpin structure. The presence of this hairpin structure sequence in the 3' untranslated region (UTR) of TSWV mRNAs implies a possible role in translation.",

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T1 - Analysis of the Tomato spotted wilt virus ambisense S RNA-encoded hairpin structure in translation

AU - Geerts-Dimitriadou, C.

AU - Lu, Y.Y.

AU - Geertsema, C.

AU - Goldbach, R.W.

AU - Kormelink, R.J.M.

PY - 2012

Y1 - 2012

N2 - The intergenic region (IR) of ambisense RNA segments from animal- and plant-infecting (-)RNA viruses functions as a bidirectional transcription terminator. The IR sequence of the Tomato spotted wilt virus (TSWV) ambisense S RNA contains stretches that are highly rich in A-residues and U-residues and is predicted to fold into a stable hairpin structure. The presence of this hairpin structure sequence in the 3' untranslated region (UTR) of TSWV mRNAs implies a possible role in translation.

AB - The intergenic region (IR) of ambisense RNA segments from animal- and plant-infecting (-)RNA viruses functions as a bidirectional transcription terminator. The IR sequence of the Tomato spotted wilt virus (TSWV) ambisense S RNA contains stretches that are highly rich in A-residues and U-residues and is predicted to fold into a stable hairpin structure. The presence of this hairpin structure sequence in the 3' untranslated region (UTR) of TSWV mRNAs implies a possible role in translation.

KW - nonstructural protein nss

KW - affecting transcription termination

KW - tail-independent translation

KW - cap-snatching mechanism

KW - escherichia-coli rna

KW - stem-loop structure

KW - cdna-based rescue

KW - messenger-rna

KW - influenza-virus

KW - intergenic region

U2 - 10.1371/journal.pone.0031013

DO - 10.1371/journal.pone.0031013

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VL - 7

JO - PLoS ONE

JF - PLoS ONE

SN - 1932-6203

IS - 2

M1 - e31013

ER -

Analysis of the Tomato spotted wilt virus ambisense S RNA-encoded hairpin structure in translation

Abstract

Keywords

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