The amounts and ratios of the four constituents of the sex pheromone gland of the moth Symmetrischema tangolias were measured during a 24-hr dark–light cycle. A new approach was followed that involved the direct introduction of sex pheromone glands into the liner of a two-dimensional gas chromatograph (2D-GC) by using a special temperature programmable GC injector. With this method it was possible to examine individual glands without prior processing. This provides a clear advantage over other methods. The detection limit was 0.5 ng. The total amount of sex pheromone in the glands varied strongly from individual to individual (3.8–350 ng/female). The total sex pheromone amounts were significantly lower in the scotophase than in the photophase (62.2 and 101.5 ng/female, respectively). The ratios of the various gland constituents showed a symmetrical distribution, which did not fluctuate during the 24-hr dark–light cycle.
|Journal||Journal of Chemical Ecology|
|Publication status||Published - 1999|
Griepink, F. C., Drijfhout, F. P., van Beek, T. A., Visser, J. H., & de Groot, Æ. (1999). Analysis of the sex pheromone gland content of individual Symmetrischema tangolias by means of direct gland introduction into a two-dimensional gas chromatograph. Journal of Chemical Ecology, 26(4).