Analysis of taxines in Taxus plant material and cell cultures by hplc photodiode array and hplc-electrospray mass spectrometry

G. Theodoridis, G. Laskaris, E.L.M. Rozendaal, R. Verpoorte

Research output: Contribution to journalArticleAcademicpeer-review

12 Citations (Scopus)

Abstract

A semi-purified Taxus baccata needles extract was analysed by RP-HPLC. More than 18 taxines and cinnamates were detected by photodiode array detection and LC-MS, 10 of them being positively identified. Furthermore, 10-deacetyl baccatin III (paclitaxel's main precursor) and other taxanes were also found in the extract. Taxines were also detected in numerous extracts of plant and cell culture extracts of various origins. The identification of the taxines in the extracts was made by correlation of retention and spectral data. LC-electrospray MS verified the identification of the known taxines in T. baccata seeds, needles, and pollen
Original languageEnglish
Pages (from-to)2267-2282
JournalJournal of liquid chromatography & related technologies
Volume24
Issue number15
DOIs
Publication statusPublished - 2001

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