Analysis of omega-3 fatty acid derived N-acylethanolamines in biological matrices

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

2 Citations (Scopus)

Abstract

The adequate quantification of endocannabinoids can be complex due to their low endogenous levels and structural diversity. Therefore, advanced analytical approaches, such as LC-MS, are used to measure endocannabinoids in plasma, tissues, and other matrices. Recent work has shown that endocannabinoids that are synthesized from n-3 fatty acids, such as docosahexaenoylethanolamide (DHEA) and eicosapentaenoylethanolamide (EPEA), have anti-inflammatory and anti-tumorigenic properties and stimulate synapse formation in neurites. Here, an LC-MS based method for the quantification of n-3 endocannabinoids DHEA and EPEA which is also suited to measure a wider spectrum of endocannabinoids is described. The chapter contains a step-by-step protocol for the analysis of n-3 endocannabinoids in plasma, including sample collection and solid phase extraction, LC-MS analysis, and data processing. Modifications to the protocol that allow quantifying n-3 endocannabinoids in tissues and cell culture media will also be discussed. Finally, conditions that alter endocannabinoid concentrations are briefly discussed.

Original languageEnglish
Title of host publicationEndocannabinoid signaling: Methods and Protocols
EditorsMauro Maccarrone
PublisherHumana Press Inc.
Pages27-40
Volume1412
ISBN (Electronic)9781493935390
ISBN (Print)9781493935376
DOIs
Publication statusPublished - 2016

Publication series

NameMethods in Molecular Biology
Volume1412
ISSN (Print)1064-3745

Fingerprint

Endocannabinoids
Omega-3 Fatty Acids
N-acylethanolamines
Solid Phase Extraction
Neurites
Synapses
Culture Media
Anti-Inflammatory Agents
Cell Culture Techniques

Keywords

  • Docosahexaenoylethanolamide
  • Eicosapentaenoylethanolamide
  • Endocannabinoids
  • LC-MS
  • N-3 fatty acids
  • Solid phase extraction

Cite this

Witkamp, R. F., & Balvers, M. (2016). Analysis of omega-3 fatty acid derived N-acylethanolamines in biological matrices. In M. Maccarrone (Ed.), Endocannabinoid signaling: Methods and Protocols (Vol. 1412, pp. 27-40). (Methods in Molecular Biology; Vol. 1412). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-3539-0_4
Witkamp, Renger F. ; Balvers, Michiel. / Analysis of omega-3 fatty acid derived N-acylethanolamines in biological matrices. Endocannabinoid signaling: Methods and Protocols. editor / Mauro Maccarrone. Vol. 1412 Humana Press Inc., 2016. pp. 27-40 (Methods in Molecular Biology).
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Witkamp, RF & Balvers, M 2016, Analysis of omega-3 fatty acid derived N-acylethanolamines in biological matrices. in M Maccarrone (ed.), Endocannabinoid signaling: Methods and Protocols. vol. 1412, Methods in Molecular Biology, vol. 1412, Humana Press Inc., pp. 27-40. https://doi.org/10.1007/978-1-4939-3539-0_4

Analysis of omega-3 fatty acid derived N-acylethanolamines in biological matrices. / Witkamp, Renger F.; Balvers, Michiel.

Endocannabinoid signaling: Methods and Protocols. ed. / Mauro Maccarrone. Vol. 1412 Humana Press Inc., 2016. p. 27-40 (Methods in Molecular Biology; Vol. 1412).

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

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AB - The adequate quantification of endocannabinoids can be complex due to their low endogenous levels and structural diversity. Therefore, advanced analytical approaches, such as LC-MS, are used to measure endocannabinoids in plasma, tissues, and other matrices. Recent work has shown that endocannabinoids that are synthesized from n-3 fatty acids, such as docosahexaenoylethanolamide (DHEA) and eicosapentaenoylethanolamide (EPEA), have anti-inflammatory and anti-tumorigenic properties and stimulate synapse formation in neurites. Here, an LC-MS based method for the quantification of n-3 endocannabinoids DHEA and EPEA which is also suited to measure a wider spectrum of endocannabinoids is described. The chapter contains a step-by-step protocol for the analysis of n-3 endocannabinoids in plasma, including sample collection and solid phase extraction, LC-MS analysis, and data processing. Modifications to the protocol that allow quantifying n-3 endocannabinoids in tissues and cell culture media will also be discussed. Finally, conditions that alter endocannabinoid concentrations are briefly discussed.

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Witkamp RF, Balvers M. Analysis of omega-3 fatty acid derived N-acylethanolamines in biological matrices. In Maccarrone M, editor, Endocannabinoid signaling: Methods and Protocols. Vol. 1412. Humana Press Inc. 2016. p. 27-40. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-3539-0_4