Analysis of OJIP transients during photoinactivation of photosystem ii indicates the presence of multiple photosensitizers in vivo and in vitro

I. Iermak, M. Szabó, A. Zavafer*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Generally, excessive excitation absorbed by the pigments is considered the cause of PSII photodamage. Previous studies of action spectra of PSII photodamage concluded that shorter wavelengths induce more damage, supporting the hypothesis of the existence of more than one photosensitizer. However, the relative influence of different photosensitizers is still inconclusive. In this work, we have revisited this question by inducing PSII photodamage in vivo and in vitro at two different wavelengths (460 and 660 nm) where the net absorption cross section was the same using equal irradiance. To correlate PSII photodamage with each wavelength band, we followed its time course using the OJIP transient of the chlorophyll fluorescence to determine the possible contributions of photoinhibition by different photosensitizers. We found evidence that at least two sites of photoinactivation of PSII exist.

Original languageEnglish
Pages (from-to)497-506
Number of pages10
JournalPhotosynthetica
Volume58
Issue numberSpecial Issue
DOIs
Publication statusPublished - 2020

Keywords

  • Additional absorptance
  • Chlorophyll fluorescence
  • Manganese cluster
  • Photodamage
  • Spinach
  • Two-photon excitation microscopy

Fingerprint Dive into the research topics of 'Analysis of OJIP transients during photoinactivation of photosystem ii indicates the presence of multiple photosensitizers in vivo and in vitro'. Together they form a unique fingerprint.

  • Cite this