Analysis of natural allelic variation of Arabidopsis seed germination and seed longevity traits between the accessions Landberg erecta and Shakdara, using a new recombinant inbred line population

E.J.M. Clerkx, M.E.M. El-Lithy, E. Vierling, G.J. Ruijs, M.H.C. de Vries, S.P.C. Groot, D. Vreugdenhil, M. Koornneef

Research output: Contribution to journalArticleAcademicpeer-review

165 Citations (Scopus)

Abstract

Quantitative trait loci (QTL) mapping was used to identify loci controlling various aspects of seed longevity during storage and germination. Similar locations for QTLs controlling different traits might be an indication for a common genetic control of such traits. For this analysis we used a new recombinant inbred line population derived from a cross between the accessions Landsberg erecta (Ler) and Shakdara (Sha). A set of 114 F9 recombinant inbred lines was genotyped with 65 polymerase chain reaction-based markers and the phenotypic marker erecta. The traits analyzed were dormancy, speed of germination, seed sugar content, seed germination after a controlled deterioration test, hydrogen peroxide (H2O2) treatment, and on abscisic acid. Furthermore, the effects of heat stress, salt (NaCl) stress, osmotic (mannitol) stress, and natural aging were analyzed. For all traits one or more QTLs were identified, with some QTLs for different traits colocating. The relevance of colocation for mechanisms underlying the various traits is discussed.
Original languageEnglish
Pages (from-to)432-443
JournalPlant Physiology
Volume135
Issue number1
DOIs
Publication statusPublished - 2004

Keywords

  • abscisic-acid
  • desiccation tolerance
  • molecular dissection
  • barley aleurone
  • cell-death
  • thaliana
  • loci
  • dormancy
  • mutants
  • quality

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