Analysis of initial chlorophyll fluorescence induction kinetics in chloroplasts in terms of rate constants of donor side quenching release and electron trapping in photosystem II

W.J. Vredenberg

Research output: Contribution to journalArticleAcademicpeer-review

28 Citations (Scopus)

Abstract

The fluorescence induction F(t) of dark-adapted chloroplasts has been studied in multi-turnover 1 s light flashes (MTFs). A theoretical expression for the initial fluorescence rise is derived from a set of rate equations that describes the sequence of transfer steps associated with the reduction of the primary quinone acceptor Q A and the release of photochemical fluorescence quenching of photosystem II (PSII). The initial F(t) rise in the hundreds of ¿s time range is shown to follow the theoretical function dictated by the rate constants of light excitation (k L) and release of donor side quenching (k si ). The bi-exponential function shows sigmoidicity when one of the two rate constants differs by less than one order of magnitude from the other. It is shown, in agreement with the theory, that the sigmoidicity of the fluorescence rise is variable with light intensity and mainly, if not exclusively, determined by the ratio between rate of light excitation and the rate constant of donor side quenching release.
Original languageEnglish
Pages (from-to)83-97
JournalPhotosynthesis Research
Volume96
Issue number1
DOIs
Publication statusPublished - 2008

Keywords

  • radical-pair equilibrium
  • restricted energy-transfer
  • single-turnover flash
  • a fluorescence
  • plastoquinone pool
  • yield changes
  • inhibiting herbicides
  • photosynthetic units
  • thylakoid membranes
  • excitation-energy

Fingerprint Dive into the research topics of 'Analysis of initial chlorophyll fluorescence induction kinetics in chloroplasts in terms of rate constants of donor side quenching release and electron trapping in photosystem II'. Together they form a unique fingerprint.

  • Cite this