Analysis of inheritance mode in chrysanthemum using EST-derived SSR markers

Sang Kun Park, Paul Arens*, Danny Esselink, Jin Hee Lim, Hak Ki Shin

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

6 Citations (Scopus)

Abstract

To study the inheritance mode of hexaploid chrysanthemum (random or preferential chromosome pairing), a segregation analysis was carried out using SSR markers derived from chrysanthemum ESTs in the public domain. A total of 248 EST-SSR primer pairs were screened in chrysanthemum cultivars 'Dancer' and 'Puma White', of which 49 EST-SSRs were selected as polymorphic and informative markers. These polymorphic markers were used for genotyping a F1-pseudo test cross population derived from a cross between these two cultivars. The 49 EST-SSRs detected 210 marker alleles with an average number of 4.29 marker alleles per locus. For 180 of these polymorphic SSR marker alleles, segregation could be estimated using a χ2 goodness of fit test (α=0.05) with the expected segregation ratios for hexasomic or disomic inheritance. For 65 SSR marker alleles the segregation ratio was informative for the type of inheritance, 33 marker alleles gave a good fit to the expected segregation ratio for hexasomic inheritance and whereas 24 marker alleles gave a good fit for disomic inheritance showing a higher number of marker alleles supporting autopolyploid segregation in chrysanthemum. In addition, the observed ratio of non-simplex to simplex markers was 20:80 (25 vs. 99) supported hexasomic inheritance. Furthermore, random marker allele assortment was found within the six fully informative markers giving conclusive evidence for hexasomic inheritance in chrysanthemum at these chromosomal regions.

Original languageEnglish
Pages (from-to)80-88
JournalScientia Horticulturae
Volume192
DOIs
Publication statusPublished - 2015

Keywords

  • Allele dosage
  • Chrysanthemum x morifolium
  • Dendranthema x grandiflorum
  • Genotyping
  • Polyploidy
  • Segregation type

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