Analysis of anthraquinones in Rubia tinctorum L. by liquid chromatography coupled with diode-array UV and mass spectrometric detection

G.C.H. Derksen; H.A.G. Niederländer; T.A. van Beek

doi:10.1016/S0021-9673(02)01412-7

Analysis of anthraquinones in Rubia tinctorum L. by liquid chromatography coupled with diode-array UV and mass spectrometric detection

G.C.H. Derksen, H.A.G. Niederländer, T.A. van Beek

Research output: Contribution to journal › Article › Academic › peer-review

122 Citations (Scopus)

Abstract

A liquid chromatographic (LC) method for the separation of both anthraquinone glycosides and aglycones in extracts of Rubia tinctorum was improved. For on-line MS detection atmospheric pressure chemical ionisation as well as electrospray ionisation (ESI) were used. The glycosides were ionised in both positive and negative ionisation (NI) mode, the aglycones only in the NI mode. With ESI ammonia was added to the eluent post-column to deprotonate the compounds. The efficiency of mass detection of the hydroxyanthraquinone aglycones was found to depend on the pKa value of the component. LC–diode-array detection and LC–MS provide useful complementary information for the identification of anthraquinones in plant extracts, which was proven with the identification of munjistin and pseudopurpurin.

Original language	English
Pages (from-to)	119-127
Journal	Journal of Chromatography. A, Including electrophoresis and other separation methods
Volume	978
DOIs	https://doi.org/10.1016/S0021-9673(02)01412-7
Publication status	Published - 2002

Keywords

Aglycones
Alizarin
Anthraquinones
Glycosides
Mass spectrometry
Plant materials
Rubia tinctorum

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10.1016/S0021-9673(02)01412-7

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@article{b951c072daae42189b82097c47c7d22c,

title = "Analysis of anthraquinones in Rubia tinctorum L. by liquid chromatography coupled with diode-array UV and mass spectrometric detection",

abstract = "A liquid chromatographic (LC) method for the separation of both anthraquinone glycosides and aglycones in extracts of Rubia tinctorum was improved. For on-line MS detection atmospheric pressure chemical ionisation as well as electrospray ionisation (ESI) were used. The glycosides were ionised in both positive and negative ionisation (NI) mode, the aglycones only in the NI mode. With ESI ammonia was added to the eluent post-column to deprotonate the compounds. The efficiency of mass detection of the hydroxyanthraquinone aglycones was found to depend on the pKa value of the component. LC–diode-array detection and LC–MS provide useful complementary information for the identification of anthraquinones in plant extracts, which was proven with the identification of munjistin and pseudopurpurin.",

keywords = "Aglycones, Alizarin, Anthraquinones, Glycosides, Mass spectrometry, Plant materials, Rubia tinctorum",

author = "G.C.H. Derksen and H.A.G. Niederl{\"a}nder and \{van Beek\}, T.A.",

year = "2002",

doi = "10.1016/S0021-9673(02)01412-7",

language = "English",

volume = "978",

pages = "119--127",

journal = "Journal of Chromatography. A, Including electrophoresis and other separation methods",

issn = "0021-9673",

publisher = "Elsevier",

}

Analysis of anthraquinones in Rubia tinctorum L. by liquid chromatography coupled with diode-array UV and mass spectrometric detection. / Derksen, G.C.H.; Niederländer, H.A.G.; van Beek, T.A.
In: Journal of Chromatography. A, Including electrophoresis and other separation methods, Vol. 978, 2002, p. 119-127.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Analysis of anthraquinones in Rubia tinctorum L. by liquid chromatography coupled with diode-array UV and mass spectrometric detection

AU - Derksen, G.C.H.

AU - Niederländer, H.A.G.

AU - van Beek, T.A.

PY - 2002

Y1 - 2002

N2 - A liquid chromatographic (LC) method for the separation of both anthraquinone glycosides and aglycones in extracts of Rubia tinctorum was improved. For on-line MS detection atmospheric pressure chemical ionisation as well as electrospray ionisation (ESI) were used. The glycosides were ionised in both positive and negative ionisation (NI) mode, the aglycones only in the NI mode. With ESI ammonia was added to the eluent post-column to deprotonate the compounds. The efficiency of mass detection of the hydroxyanthraquinone aglycones was found to depend on the pKa value of the component. LC–diode-array detection and LC–MS provide useful complementary information for the identification of anthraquinones in plant extracts, which was proven with the identification of munjistin and pseudopurpurin.

AB - A liquid chromatographic (LC) method for the separation of both anthraquinone glycosides and aglycones in extracts of Rubia tinctorum was improved. For on-line MS detection atmospheric pressure chemical ionisation as well as electrospray ionisation (ESI) were used. The glycosides were ionised in both positive and negative ionisation (NI) mode, the aglycones only in the NI mode. With ESI ammonia was added to the eluent post-column to deprotonate the compounds. The efficiency of mass detection of the hydroxyanthraquinone aglycones was found to depend on the pKa value of the component. LC–diode-array detection and LC–MS provide useful complementary information for the identification of anthraquinones in plant extracts, which was proven with the identification of munjistin and pseudopurpurin.

KW - Aglycones

KW - Alizarin

KW - Anthraquinones

KW - Glycosides

KW - Mass spectrometry

KW - Plant materials

KW - Rubia tinctorum

U2 - 10.1016/S0021-9673(02)01412-7

DO - 10.1016/S0021-9673(02)01412-7

M3 - Article

SN - 0021-9673

VL - 978

SP - 119

EP - 127

JO - Journal of Chromatography. A, Including electrophoresis and other separation methods

JF - Journal of Chromatography. A, Including electrophoresis and other separation methods

ER -

Analysis of anthraquinones in Rubia tinctorum L. by liquid chromatography coupled with diode-array UV and mass spectrometric detection

Abstract

Keywords

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