Analysis of acid-stressed Bacillus cereus reveals a major oxidative response and inactivation-associated radical formation

J.M. Mols, R. van Kranenburg, C.C.J. van Melis, R. Moezelaar, T. Abee

Research output: Contribution to journalArticleAcademicpeer-review

69 Citations (Scopus)

Abstract

Acid stress resistance of the food-borne human pathogen Bacillus cereus may contribute to its survival in acidic environments, such as encountered in soil, food and the human gastrointestinal tract. The acid stress responses of B. cereus strains ATCC 14579 and ATCC 10987 were analysed in aerobically grown cultures acidified to pH values ranging from pH 5.4 to pH 4.4 with HCI. Comparative phenotype and transcriptome analyses revealed three acid stressinduced responses in this pH range: growth rate reduction, growth arrest and loss of viability. These physiological responses showed to be associated with metabolic shifts and the induction of general stress response mechanisms with a major oxidative component, including upregulation of catalases and superoxide dismutases. Flow cytometry analysis in combination with the hydroxyl (OH center dot) and peroxynitrite (ONOO-)-specific fluorescent probe 3'-(phydroxyphenyl) fluorescein (HPF) showed excessive radicals to be formed in both B. cereus strains in bactericidal conditions only. Our study shows that radicals can indicate acid-induced malfunctioning of cellular processes that lead to cell death.
Original languageEnglish
Pages (from-to)873-885
JournalEnvironmental Microbiology
Volume12
Issue number4
DOIs
Publication statusPublished - 2010

Fingerprint

Bacillus cereus
inactivation
Acids
acids
acid
stress response
stress resistance
Food
Peroxynitrous Acid
food
flow cytometry
Gene Expression Profiling
physiological response
fluorescein
Growth
Fluorescein
Fluorescent Dyes
transcriptome
Hydroxyl Radical
stress tolerance

Keywords

  • arginine deiminase system
  • food poisoning toxins
  • nitric-oxide
  • listeria-monocytogenes
  • staphylococcus-aureus
  • superoxide-dismutase
  • tolerance response
  • lactococcus-lactis
  • genome sequence
  • low-ph

Cite this

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title = "Analysis of acid-stressed Bacillus cereus reveals a major oxidative response and inactivation-associated radical formation",
abstract = "Acid stress resistance of the food-borne human pathogen Bacillus cereus may contribute to its survival in acidic environments, such as encountered in soil, food and the human gastrointestinal tract. The acid stress responses of B. cereus strains ATCC 14579 and ATCC 10987 were analysed in aerobically grown cultures acidified to pH values ranging from pH 5.4 to pH 4.4 with HCI. Comparative phenotype and transcriptome analyses revealed three acid stressinduced responses in this pH range: growth rate reduction, growth arrest and loss of viability. These physiological responses showed to be associated with metabolic shifts and the induction of general stress response mechanisms with a major oxidative component, including upregulation of catalases and superoxide dismutases. Flow cytometry analysis in combination with the hydroxyl (OH center dot) and peroxynitrite (ONOO-)-specific fluorescent probe 3'-(phydroxyphenyl) fluorescein (HPF) showed excessive radicals to be formed in both B. cereus strains in bactericidal conditions only. Our study shows that radicals can indicate acid-induced malfunctioning of cellular processes that lead to cell death.",
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Analysis of acid-stressed Bacillus cereus reveals a major oxidative response and inactivation-associated radical formation. / Mols, J.M.; van Kranenburg, R.; van Melis, C.C.J.; Moezelaar, R.; Abee, T.

In: Environmental Microbiology, Vol. 12, No. 4, 2010, p. 873-885.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

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AU - van Kranenburg, R.

AU - van Melis, C.C.J.

AU - Moezelaar, R.

AU - Abee, T.

PY - 2010

Y1 - 2010

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AB - Acid stress resistance of the food-borne human pathogen Bacillus cereus may contribute to its survival in acidic environments, such as encountered in soil, food and the human gastrointestinal tract. The acid stress responses of B. cereus strains ATCC 14579 and ATCC 10987 were analysed in aerobically grown cultures acidified to pH values ranging from pH 5.4 to pH 4.4 with HCI. Comparative phenotype and transcriptome analyses revealed three acid stressinduced responses in this pH range: growth rate reduction, growth arrest and loss of viability. These physiological responses showed to be associated with metabolic shifts and the induction of general stress response mechanisms with a major oxidative component, including upregulation of catalases and superoxide dismutases. Flow cytometry analysis in combination with the hydroxyl (OH center dot) and peroxynitrite (ONOO-)-specific fluorescent probe 3'-(phydroxyphenyl) fluorescein (HPF) showed excessive radicals to be formed in both B. cereus strains in bactericidal conditions only. Our study shows that radicals can indicate acid-induced malfunctioning of cellular processes that lead to cell death.

KW - arginine deiminase system

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KW - nitric-oxide

KW - listeria-monocytogenes

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KW - superoxide-dismutase

KW - tolerance response

KW - lactococcus-lactis

KW - genome sequence

KW - low-ph

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